2025
IL‐6 signaling regulates the inflammatory response without impacting pathogen burden during influenza‐associated pulmonary aspergillosis
Sharma L, Singh R, Tolman N, Ngeow C, Duray A, Naghshtabrizi N, Ahmad A, Bain W, Robinson K. IL‐6 signaling regulates the inflammatory response without impacting pathogen burden during influenza‐associated pulmonary aspergillosis. Physiological Reports 2025, 13: e70372. PMID: 40420617, PMCID: PMC12106949, DOI: 10.14814/phy2.70372.Peer-Reviewed Original ResearchConceptsAF infectionAspergillus fumigatusInterleukin-6IL-6 signalingLung inflammationInfluenza-associated pulmonary aspergillosisInterleukin-6 knockout miceNeutrophilic lung inflammationBronchoalveolar lavage fluidEpithelial cell damageLung capillary permeabilitySusceptibility to opportunistic pathogensPulmonary aspergillosisClinical courseLavage fluidKnockout miceOpportunistic pathogenRAGE levelsEpithelial integrityMouse modelIL-6Pathological inflammationTissue injuryInflammatory responseViral infectionRapid Metagenomic Sequencing (RaMSes) of Bronchoalveolar Lavage Fluid for Diagnosis of Infection in Patients with Hematologic Malignancies and Pulmonary Complications
Hensley M, Sayed K, Haidar G, Wang X, Benos P, Ito S, Im A, Geramita E, Shlomchik W, Methé B, Dela Cruz C, Morris A, Kitsios G. Rapid Metagenomic Sequencing (RaMSes) of Bronchoalveolar Lavage Fluid for Diagnosis of Infection in Patients with Hematologic Malignancies and Pulmonary Complications. CHEST Pulmonary 2025, 100173. DOI: 10.1016/j.chpulm.2025.100173.Peer-Reviewed Original ResearchPulmonary complicationsHematologic malignanciesRespiratory pathogensCulture-independent sequencing approachesDetection of fungal pathogensNon-infectious pulmonary complicationsSuspected lower respiratory tract infectionLower respiratory tract infectionsCulture-independent sequencing methodsRespiratory tract infectionsBronchoalveolar lavage fluidDiagnosis of infectionEmpirical antimicrobialsPCR-based testsTract infectionsFungal pathogensMetagenomic sequencingClinical manifestationsSequencing approachLavage fluidMicrobial communitiesCohort studySequencing methodsPatientsComplications
2023
Maresin1 prevents sepsis-induced acute liver injury by suppressing NF-κB/Stat3/MAPK pathways, mitigating inflammation
Sun S, Wang L, Wang J, Chen R, Pei S, Yao S, Lin Y, Yao C, Xia H. Maresin1 prevents sepsis-induced acute liver injury by suppressing NF-κB/Stat3/MAPK pathways, mitigating inflammation. Heliyon 2023, 9: e21883. PMID: 38027581, PMCID: PMC10665730, DOI: 10.1016/j.heliyon.2023.e21883.Peer-Reviewed Original ResearchSepsis-induced acute liver injuryAcute liver injuryAnti-inflammatory effectsLiver injuryNeutrophil infiltrationBacterial loadSurvival rateDocosahexaenoic acidNormal saline 1Peritoneal lavage fluidPro-inflammatory moleculesTreatment of sepsisUncontrolled inflammatory responseAnti-inflammatory moleculesSepsis-induced injuryEffects of MaR1Body weight changesSepsis-related diseasesDose-dependent mannerSaline 1Septic miceCecal ligationLavage fluidLiver functionAcute inflammationRole of fungal burden in risk stratification of HIV-negative patients with Pneumocystis pneumonia: A 12-year, retrospective, observational, multicenter cohort
Grønseth S, Rogne T, Heggelund L, Åsvold B, Afset J, Damås J. Role of fungal burden in risk stratification of HIV-negative patients with Pneumocystis pneumonia: A 12-year, retrospective, observational, multicenter cohort. International Journal Of Infectious Diseases 2023, 134: 177-186. PMID: 37339716, DOI: 10.1016/j.ijid.2023.06.013.Peer-Reviewed Original ResearchConceptsCharlson Comorbidity IndexHIV-negative patientsPneumocystis pneumoniaRisk stratificationFungal burdenMulticenter cohortPolymerase chain reactionAbnormal leukocyte countComorbid cardiovascular diseaseLow serum albuminBronchoalveolar lavage fluidC-reactive proteinChain reactionHigher fungal burdenReal-time polymerase chain reactionProbable Pneumocystis pneumoniaCycle threshold valuesComorbidity indexCorticosteroid useLavage fluidSemiquantitative real-time polymerase chain reactionLeukocyte countImmunological disordersCardiovascular diseaseRetrospective analysis
2022
303. Viral and bacterial infections among adults hospitalized with COVID-19, Coronavirus Disease 2019-Associated Hospitalization Surveillance Network, 14 states, March 2020–February 2022
Melisa S, Taylor C, Patel K, Milucky J, Whitaker M, Pham H, Anglin O, Reingold A, Armistead I, Yousey-Hindes K, Anderson E, Weigel A, Reeg L, Mumm E, Ropp S, Muse A, Bushey S, Shiltz E, Sutton M, Talbot K, Price A, Havers F. 303. Viral and bacterial infections among adults hospitalized with COVID-19, Coronavirus Disease 2019-Associated Hospitalization Surveillance Network, 14 states, March 2020–February 2022. Open Forum Infectious Diseases 2022, 9: ofac492.381. PMCID: PMC9751616, DOI: 10.1093/ofid/ofac492.381.Peer-Reviewed Original ResearchDay of admissionBacterial infectionsCoronavirus diseaseRelevant bacterial pathogensCOVID-19Acute respiratory syndrome coronavirus 2 infectionLaboratory-confirmed SARS-CoV-2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSyndrome coronavirus 2 infectionSARS-CoV-2 infectionSurveillance networkCommon respiratory isolatesRV/EVCoronavirus 2 infectionRhinovirus/enterovirusBronchoalveolar lavage fluidRespiratory syncytial virusBacterial pathogensCare molecular testingHospitalized adultsBacterial coinfectionClinical featuresClinical outcomesLavage fluidSyncytial virusUnilateral Pneumonitis and Hydropneumothorax Following Pembrolizumab
Sanchez A, Kahn P, Minerowicz C, Kluger H. Unilateral Pneumonitis and Hydropneumothorax Following Pembrolizumab. Journal Of Brown Hospital Medicine 2022, 2: 39747. PMID: 40046551, PMCID: PMC11878863, DOI: 10.56305/001c.39747.Peer-Reviewed Case Reports and Technical NotesCheckpoint inhibitor pneumonitisTransbronchial biopsy resultsBronchoalveolar lavage fluidProgressive dyspneaDry coughPembrolizumab monotherapyLavage fluidRadiographic findingsBiopsy resultsMetastatic melanomaPleural fluidPneumonitisHydropneumothoraxPembrolizumabDyspneaMonotherapyCoughPneumoniaMelanomaSymptomsInfectionWeeksInduction of Severe Eosinophilic Esophagitis and Multi-Organ Inflammation by Airborne Allergens is Associated with IL-4/IL-13 and CCL11 but Not IgE in Genetic Susceptible Mice
Maskey A, Srivastava K, Soffer G, Dunkin D, Yuan Q, Li X. Induction of Severe Eosinophilic Esophagitis and Multi-Organ Inflammation by Airborne Allergens is Associated with IL-4/IL-13 and CCL11 but Not IgE in Genetic Susceptible Mice. Journal Of Inflammation Research 2022, 15: 5527-5540. PMID: 36176352, PMCID: PMC9514888, DOI: 10.2147/jir.s372449.Peer-Reviewed Original ResearchAirborne allergensNormal controlsEosinophilic inflammationEosinophilic esophagitisTh2 cytokinesAntigen-specific IgE levelsCommon chronic inflammatory diseaseImportant pathologic roleMild eosinophilic inflammationSevere eosinophilic esophagitisSevere eosinophilic inflammationIL-4/ILBronchoalveolar lavage fluidChronic inflammatory diseaseMulti-organ inflammationGene-deficient miceChemokine gene expressionIgA levelsIgE levelsIgG levelsSerum totalSpecific IgETotal IgAWT miceLavage fluid
2021
Distinct Immunophenotypes of T Cells in Bronchoalveolar Lavage Fluid From Leukemia Patients With Immune Checkpoint Inhibitors-Related Pulmonary Complications
Kim ST, Sheshadri A, Shannon V, Kontoyiannis DP, Kantarjian H, Garcia-Manero G, Ravandi F, Im JS, Boddu P, Bashoura L, Balachandran DD, Evans SE, Faiz S, Vazquez W, Divenko M, Mathur R, Tippen SP, Gumbs C, Neelapu SS, Naing A, Wang L, Diab A, Futreal A, Nurieva R, Daver N. Distinct Immunophenotypes of T Cells in Bronchoalveolar Lavage Fluid From Leukemia Patients With Immune Checkpoint Inhibitors-Related Pulmonary Complications. Frontiers In Immunology 2021, 11: 590494. PMID: 33552049, PMCID: PMC7859512, DOI: 10.3389/fimmu.2020.590494.Peer-Reviewed Original ResearchConceptsImmune checkpoint inhibitorsAML/MDS patientsPulmonary complicationsAcute myeloid leukemiaMDS patientsICI groupCheckpoint inhibitorsBronchoalveolar lavageMyelodysplastic syndromeT cellsTh17/Th1 cellsBAL T cellsRisk of pneumonitisBronchoalveolar lavage fluidFungal pneumoniaPulmonary symptomsIL-17Lavage fluidPeripheral bloodTh1 cellsLeukemia patientsMyeloid leukemiaDistinct immunophenotypePatientsComplications
2020
Inhaled multi-walled carbon nanotubes differently modulate global gene and protein expression in rat lungs
Seidel C, Zhernovkov V, Cassidy H, Kholodenko B, Matallanas D, Cosnier F, Gaté L. Inhaled multi-walled carbon nanotubes differently modulate global gene and protein expression in rat lungs. Nanotoxicology 2020, 15: 238-256. PMID: 33332178, DOI: 10.1080/17435390.2020.1851418.Peer-Reviewed Original ResearchConceptsNM-401Bronchoalveolar lavage fluidLong-term health effectsWeeks of inhalationDose-dependent increasePost-exposure timeLung inflammationLavage fluidWhole lungRat lungInhalationHealth effectsProtein expressionRecovery periodMechanisms of toxicityMWCNT exposureDifferent toxicological profilesLungToxicological profileDaysToxicological effectsPersistent effectsToxicityAnimalsToxicogenomic approachDiagnosis of Hypersensitivity Pneumonitis in Adults. An Official ATS/JRS/ALAT Clinical Practice Guideline
Raghu G, Remy-Jardin M, Ryerson CJ, Myers JL, Kreuter M, Vasakova M, Bargagli E, Chung JH, Collins BF, Bendstrup E, Chami HA, Chua AT, Corte TJ, Dalphin JC, Danoff SK, Diaz-Mendoza J, Duggal A, Egashira R, Ewing T, Gulati M, Inoue Y, Jenkins AR, Johannson KA, Johkoh T, Tamae-Kakazu M, Kitaichi M, Knight SL, Koschel D, Lederer DJ, Mageto Y, Maier LA, Matiz C, Morell F, Nicholson AG, Patolia S, Pereira CA, Renzoni EA, Salisbury ML, Selman M, Walsh SLF, Wuyts WA, Wilson KC. Diagnosis of Hypersensitivity Pneumonitis in Adults. An Official ATS/JRS/ALAT Clinical Practice Guideline. American Journal Of Respiratory And Critical Care Medicine 2020, 202: e36-e69. PMID: 32706311, PMCID: PMC7397797, DOI: 10.1164/rccm.202005-2032st.Peer-Reviewed Original ResearchConceptsSurgical lung biopsyHypersensitivity pneumonitisLung biopsyGuidelines CommitteeDiagnosis of HPFibrotic hypersensitivity pneumonitisNonfibrotic hypersensitivity pneumonitisTransbronchial lung biopsyTransbronchial lung cryobiopsyInterstitial lung diseaseJapanese Respiratory SocietyBronchoalveolar lavage fluidClinical practice guidelinesAmerican Thoracic SocietyPotential exposureGRADE approachLung cryobiopsyLavage fluidSerum IgGLung diseasePathological featuresThorough historyRespiratory SocietyThoracic SocietyPractice guidelinesA mechanistic model and therapeutic interventions for COVID-19 involving a RAS-mediated bradykinin storm
Garvin M, Alvarez C, Miller JI, Prates ET, Walker AM, Amos BK, Mast AE, Justice A, Aronow B, Jacobson D. A mechanistic model and therapeutic interventions for COVID-19 involving a RAS-mediated bradykinin storm. ELife 2020, 9: e59177. PMID: 32633718, PMCID: PMC7410499, DOI: 10.7554/elife.59177.Peer-Reviewed Original ResearchConceptsBronchoalveolar lavage fluidCOVID-19COVID-19 patientsBradykinin stormRAS receptorsLavage fluidVasopressor systemsBradykinin levelsVascular dilationVascular permeabilityBradykinin receptorsTherapeutic interventionsTherapeutic intervention pointNovel molecular mechanismDecreased expressionACE2Atypical patternsDisease mechanismsHypotensionAngiotensinMultiple tissuesCritical imbalanceReceptorsMolecular mechanismsVirusReduced Sialylation and Bioactivity of the Antifibrotic Protein Serum Amyloid P in the Sera of Patients with Idiopathic Pulmonary Fibrosis
Chen W, Karhadkar TR, Ryu C, Herzog EL, Gomer RH. Reduced Sialylation and Bioactivity of the Antifibrotic Protein Serum Amyloid P in the Sera of Patients with Idiopathic Pulmonary Fibrosis. ImmunoHorizons 2020, 4: 352-362. PMID: 32576593, PMCID: PMC8500545, DOI: 10.4049/immunohorizons.2000043.Peer-Reviewed Original ResearchConceptsSerum amyloid PIPF patientsPulmonary fibrosisFibrocyte differentiationIL-10 accumulationAmyloid PEffects of SAPIdiopathic pulmonary fibrosis (IPF) pathogenesisPulmonary fibrosis pathogenesisIdiopathic pulmonary fibrosisBronchoalveolar lavage fluidSera of patientsHigh extracellular levelsPotential therapeutic targetDifferentiation of monocytesSialic acidIPF pathogenesisIL-10Scar-like tissueLavage fluidHealthy controlsFatal disorderFibrosis pathogenesisHuman PBMCsTherapeutic targetGomori Methenamine Silver Stain on Bronchoalveolar Lavage Fluid Is Poorly Sensitive for Diagnosis of Pneumocystis jiroveci Pneumonia in HIV-Negative Immunocompromised Patients and May Lead to Missed or Delayed Diagnoses
Azar MM, Slotkin R, Abi-Raad R, Liu Y, Grant MH, Malinis MF. Gomori Methenamine Silver Stain on Bronchoalveolar Lavage Fluid Is Poorly Sensitive for Diagnosis of Pneumocystis jiroveci Pneumonia in HIV-Negative Immunocompromised Patients and May Lead to Missed or Delayed Diagnoses. Archives Of Pathology & Laboratory Medicine 2020, 144: 1003-1010. PMID: 31904277, DOI: 10.5858/arpa.2019-0394-oa.Peer-Reviewed Original ResearchHIV-negative immunocompromised patientsBronchoalveolar lavage fluidDiagnosis of PJPHIV-positive patientsImmunocompromised patientsLavage fluidGomori methenamine silver stainingPneumocystis jiroveci pneumoniaHIV-positive casesHuman immunodeficiency virusGomori methenamine silver stainMethenamine silver stainingMethenamine silver stainJiroveci pneumoniaBAL fluidHistorical gold standardRetrospective reviewImmunodeficiency virusPatientsLow burdenDiagnosisGMS stainGold standardPJPPneumonia
2019
Respiratory Virus Detection in Nasal Lavage Fluid of Chronic Rhinosinusitis Patients During Acute Exacerbations
Kwah J, Benjamin M, Fischer S, Biyasheva A, Hadzic A, Kato A, Stevens W, Kern R, Smith S, Avila P, Welch K, Conley D, Tan B, Grammer L, Schleimer R, Peters A. Respiratory Virus Detection in Nasal Lavage Fluid of Chronic Rhinosinusitis Patients During Acute Exacerbations. Journal Of Allergy And Clinical Immunology 2019, 143: ab86. PMCID: PMC7133156, DOI: 10.1016/j.jaci.2018.12.266.Peer-Reviewed Original Research
2018
Regulation and Role of Chitotriosidase during Lung Infection with Klebsiella pneumoniae
Sharma L, Amick AK, Vasudevan S, Lee SW, Marion CR, Liu W, Brady V, Losier A, Bermejo SD, Britto CJ, Lee CG, Elias JA, Dela Cruz CS. Regulation and Role of Chitotriosidase during Lung Infection with Klebsiella pneumoniae. The Journal Of Immunology 2018, 201: 615-626. PMID: 29891554, PMCID: PMC6291403, DOI: 10.4049/jimmunol.1701782.Peer-Reviewed Original ResearchConceptsLung infectionMouse modelRole of chitotriosidaseBronchoalveolar lavage fluidNumber of neutrophilsSimilar inflammatory responseRole of CHIT1Antibiotic therapyImproved survivalInflammatory changesLavage fluidInflammatory responseNeutrophil proteasesBacterial disseminationTrue chitinasesInfectionBeneficial effectsDetrimental roleAkt pathwayKlebsiella pneumoniaeAkt inhibitorCHIT1Chitinase-like proteinsMiceAkt activationTH1/17 hybrid CD4+ cells in bronchial alveolar lavage fluid from leukemia patients with checkpoint inhibitor-induced pneumonitis.
Kim S, Shannon V, Sheshadri A, Kantarjian H, Garcia-Manero G, Im J, Ravandi F, Naing A, Futreal A, Daver N. TH1/17 hybrid CD4+ cells in bronchial alveolar lavage fluid from leukemia patients with checkpoint inhibitor-induced pneumonitis. Journal Of Clinical Oncology 2018, 36: 204-204. DOI: 10.1200/jco.2018.36.5_suppl.204.Peer-Reviewed Original ResearchAcute myeloid leukemiaBronchial alveolar lavageMyelodysplastic syndrome patientsCD4+ cellsImmune related adverse eventsMyelodysplastic syndromeCell subsetsImmune checkpoint inhibitor (ICI)-based therapyPneumonitis groupCD4+ cell subsetsAnalyzed BAL fluidBronchial alveolar lavage fluidRegulatory T cellsRelated adverse eventsAlveolar lavage fluidLineage specific markersPD-1BAL fluidMyeloid leukemiaT cellsLeukemia patientsLung inflammationRisk stratificationAdverse eventsLavage fluidSurfactant protein C dampens inflammation by decreasing JAK/STAT activation during lung repair
Jin H, Ciechanowicz AK, Kaplan AR, Wang L, Zhang P, Lu YC, Tobin RE, Tobin BA, Cohn L, Zeiss CJ, Lee PJ, Bruscia EM, Krause DS. Surfactant protein C dampens inflammation by decreasing JAK/STAT activation during lung repair. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2018, 314: l882-l892. PMID: 29345196, PMCID: PMC6008135, DOI: 10.1152/ajplung.00418.2017.Peer-Reviewed Original ResearchConceptsAcute respiratory distress syndromeKO miceSurfactant protein CClinical acute respiratory distress syndromeProtein CAlveolar type 2 cellsAnti-inflammatory mediatorsRespiratory distress syndromeBronchoalveolar lavage fluidAnti-inflammatory moleculesPhosphorylated signal transductionType 2 cellsSPC expressionInducible suicide geneJanus kinaseLevels of suppressorDistress syndromeBAL fluidGranulocyte infiltrationJAK1/2 inhibitorLavage fluidProinflammatory phenotypeInflammatory cytokinesSevere inflammationInjury model
2017
Th1/17 Hybrid CD4+ Cells Are Expanded in Bronchial Alveolar Lavage Fluid from Leukemia Patients with Checkpoint Inhibitor-Induced Pneumonitis
Kim S, Shannon V, Sheshadri A, Kantarjian H, Garcia-Manero G, Im J, Ravandi F, Naing A, Sharma P, Kontoyiannis D, Futreal A, Daver N. Th1/17 Hybrid CD4+ Cells Are Expanded in Bronchial Alveolar Lavage Fluid from Leukemia Patients with Checkpoint Inhibitor-Induced Pneumonitis. Clinical Lymphoma Myeloma & Leukemia 2017, 17: s10. DOI: 10.1016/j.clml.2017.09.037.Peer-Reviewed Original ResearchMass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol
Hettick JM, Law BF, Lin CC, Wisnewski AV, Siegel PD. Mass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol. Xenobiotica 2017, 48: 626-636. PMID: 28629263, PMCID: PMC5864557, DOI: 10.1080/00498254.2017.1344791.Peer-Reviewed Original ResearchConceptsLavage fluidOccupational allergic respiratory diseaseBALB/c mouse modelMurine bronchoalveolar lavage fluidDiisocyanate-induced asthmaBronchoalveolar lavage fluidAllergic respiratory diseasesBronchoalveolar lavage samplesDiphenyl diisocyanate aerosolsLabel-free quantitative proteomic strategyMDI conjugationWestern blot analysisHealth burdenLavage samplesRespiratory diseaseAerosol exposureMouse modelDisease pathogenesisRespiratory exposureCausative agentBlot analysisCytochrome P450Argininosuccinate synthaseQuantitative proteomic strategyCellular fractionsReaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy
Wisnewski AV, Kanyo J, Asher J, Goodrich JA, Barnett G, Patrylak L, Liu J, Redlich CA, Nassar AF. Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy. Xenobiotica 2017, 48: 488-497. PMID: 28489470, PMCID: PMC5863241, DOI: 10.1080/00498254.2017.1329569.Peer-Reviewed Original ResearchConceptsCollision-induced dissociation (CID) fragmentation patternsReaction productsLower airwaysChemical reactivityLow molecular weight fractionWeight fractionHigh molecular weight fractionAliphatic diisocyanateMolecular weight fractionFragmentation patternsHexamethylenediisocyanateLC-MSHDI vaporAirways of rabbitsBronchoalveolar lavage fluidRabbit bronchoalveolar lavage fluidMoleculesDiisocyanate vaporsMs. 4Albumin. 5Occupational asthmaReactivityBAL fluidAsthma pathogenesisLavage fluid
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply