2016
Adenylyl cyclase 5 links changes in calcium homeostasis to cAMP-dependent cyst growth in polycystic liver disease
Spirli C, Mariotti V, Villani A, Fabris L, Fiorotto R, Strazzabosco M. Adenylyl cyclase 5 links changes in calcium homeostasis to cAMP-dependent cyst growth in polycystic liver disease. Journal Of Hepatology 2016, 66: 571-580. PMID: 27826057, PMCID: PMC5316496, DOI: 10.1016/j.jhep.2016.10.032.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl Cyclase InhibitorsAdenylyl CyclasesAnimalsCalciumCell ProliferationCyclic AMPCystsDisease Models, AnimalHomeostasisHumansLiver DiseasesMAP Kinase Signaling SystemMiceMice, KnockoutPolycystic Kidney, Autosomal DominantRNA InterferenceSignal TransductionStromal Interaction Molecule 1TRPP Cation ChannelsVascular Endothelial Growth Factor AConceptsProgressive cyst growthPolycystic liver diseaseNovel therapeutic targetLiver diseaseKO miceCyst growthTherapeutic targetBiliary organoidsDouble conditional knockout miceCAMP productionAutosomal dominant polycystic kidney diseaseVascular endothelial growth factorCell proliferationDominant polycystic kidney diseaseEndothelial growth factorConditional knockout micePolycystic kidney diseaseLiver transplantationLevels of cAMPStore-operated CaCystic areasKidney diseaseCyst sizeVivo treatmentKnockout mice
2015
Elevated follicular helper T Cells and expression of IL-21 in thyroid tissues are involved in the pathogenesis of Graves’ disease
Zhang J, Ren M, Zeng H, Guo Y, Zhuang Z, Feng Z, Yan H, Xu M, Liang W, Yang C, Cheng H, Ding H, Yan L. Elevated follicular helper T Cells and expression of IL-21 in thyroid tissues are involved in the pathogenesis of Graves’ disease. Immunologic Research 2015, 62: 163-174. PMID: 25894310, DOI: 10.1007/s12026-015-8647-z.Peer-Reviewed Original ResearchConceptsGD thyroid tissuesExpression of TfhFollicular helper T cellsThyroid follicular cellsPresence of TFHIL-21Helper T cellsThyroid tissueTSAb stimulationThyroid autoantibodiesIL-21RT cellsCAMP productionIL-21 stimulationPathogenesis of GDPathogenesis of GravesRelated factorsReal-time polymerase chain reactionNormal thyroid follicular cellsNormal thyroid tissueSerum levelsAutoimmune diseasesConfocal microscopyPolymerase chain reactionTfh
2012
Altered store operated calcium entry increases cyclic 3′,5′‐adenosine monophosphate production and extracellular signal‐regulated kinases 1 and 2 phosphorylation in polycystin‐2‐defective cholangiocytes
Spirli C, Locatelli L, Fiorotto R, Morell CM, Fabris L, Pozzan T, Strazzabosco M. Altered store operated calcium entry increases cyclic 3′,5′‐adenosine monophosphate production and extracellular signal‐regulated kinases 1 and 2 phosphorylation in polycystin‐2‐defective cholangiocytes. Hepatology 2012, 55: 856-868. PMID: 21987453, PMCID: PMC3272110, DOI: 10.1002/hep.24723.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsBile DuctsCalciumCalcium ChannelsCalcium SignalingCells, CulturedCyclic AMPCyclic AMP-Dependent Protein KinasesHomeostasisMembrane GlycoproteinsMiceMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Models, AnimalPhosphorylationSignal TransductionStromal Interaction Molecule 1TRPP Cation ChannelsVascular Endothelial Growth Factor AConceptsSensor stromal interaction molecule 1Adenylyl cyclase type 6Extracellular signal-regulated kinases 1Signal-regulated kinases 1Overproduction of cAMPStromal interaction molecule 1Orai channelsWild-type miceSOCE activationCAMP productionRapamycin (mTOR) signalingKinase 1ERK pathwayERK1/2 activationHuman diseasesWT cellsMammalian targetDependent activationSTIM-1CAMP/Inappropriate activationCyst growthCystic cholangiocytesPolycystic liver diseaseActivation
2011
The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion
Chin A, Svejda B, Gustafsson B, Granlund A, Sandvik A, Timberlake A, Sumpio B, Pfragner R, Modlin I, Kidd M. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. AJP Gastrointestinal And Liver Physiology 2011, 302: g397-g405. PMID: 22038827, PMCID: PMC3287403, DOI: 10.1152/ajpgi.00087.2011.Peer-Reviewed Original ResearchMeSH KeywordsAcetamidesAdenosineAdenosine A2 Receptor AgonistsAdenosine A2 Receptor AntagonistsAdenosine-5'-(N-ethylcarboxamide)AdultAgedCell Line, TumorCells, CulturedColonCrohn DiseaseCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEnterochromaffin CellsFemaleGene ExpressionHumansMaleMAP Kinase Kinase 1MAP Kinase Signaling SystemMechanotransduction, CellularMiddle AgedPhosphorylationProto-Oncogene Proteins c-aktPurinesReceptor, Adenosine A1Receptor, Adenosine A2AReceptor, Adenosine A2BReceptor, Adenosine A3SerotoninSignal TransductionStress, MechanicalTryptophan HydroxylaseVesicular Monoamine Transport ProteinsConceptsInflammatory bowel diseaseEC cellsCell functionEC cell functionMechanical stimulationDevelopment of agentsSecrete serotoninBowel diseaseIntracellular cAMP levelsGut motilityEnterochromaffin cellsAdenosine responsivenessReceptor agonistReceptor expressionHuman EC cellsSerotonin secretionAdenosine receptorsMRS1754CAMP productionSecretionCAMP levelsNECANeoplasiaMechanosensory cellsDisease
2009
The calcium-sensing receptor couples to Gαs and regulates PTHrP and ACTH secretion in pituitary cells
Mamillapalli R, Wysolmerski J. The calcium-sensing receptor couples to Gαs and regulates PTHrP and ACTH secretion in pituitary cells. Journal Of Endocrinology 2009, 204: 287-297. PMID: 20032198, PMCID: PMC3777408, DOI: 10.1677/joe-09-0183.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsACTH secretionCAMP productionProtein-coupled receptorsDifferent G proteinsCaR couplesAtT-20 cellsHormone secretionExtracellular calciumSystemic calcium homeostasisBiological functionsG proteinsBreast cancer cellsCell typesCalcium-sensing receptorCell growthReceptors coupleMalignant breast cellsCancer cellsKidney cellsBreast cellsCAR activationPituitary corticotrophsCalcium homeostasisAnterior pituitaryDiferentially expressed adenylyl cyclase isoforms mediate secretory functions in cholangiocyte subpopulation
Strazzabosco M, Fiorotto R, Melero S, Glaser S, Francis H, Spirli C, Alpini G. Diferentially expressed adenylyl cyclase isoforms mediate secretory functions in cholangiocyte subpopulation. Hepatology 2009, 50: 244-252. PMID: 19444869, PMCID: PMC2738985, DOI: 10.1002/hep.22926.Peer-Reviewed Original ResearchConceptsSoluble adenylyl cyclaseAdenylyl cyclasesGene expressionAC isoformsCyclic adenosine monophosphateAC gene expressionDifferent tissue specificitiesGroup of enzymesAdenylyl cyclase isoformsTissue specificityCholangiocyte secretionCyclase isoformsIsoformsSAC inhibitorIsohydric changesAdenylyl cyclaseIsoform expressionSACS geneReal-time polymerase chain reactionGenesAdenosine monophosphateAC8ExpressionCAMP levelsCAMP production
2007
Succinate is a paracrine signal for liver damage
Correa PR, Kruglov EA, Thompson M, Leite MF, Dranoff JA, Nathanson MH. Succinate is a paracrine signal for liver damage. Journal Of Hepatology 2007, 47: 262-269. PMID: 17451837, PMCID: PMC1986575, DOI: 10.1016/j.jhep.2007.03.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueIn Vitro TechniquesInfusions, IntravenousIschemiaLiverLiver DiseasesMaleParacrine CommunicationPerfusionPortal VeinPressureRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReverse Transcriptase Polymerase Chain ReactionSignal TransductionSuccinic AcidTissue DistributionConceptsHepatic stellate cellsSuccinate receptorParacrine signalsStellate cell activationStellate cellsCell expression systemTime-lapse imagingRelease of succinateCell activationCytosolic Ca2Effect of succinatePrimary hepatic stellate cellsHepatic cell typesExpression systemQuiescent hepatic stellate cellsConfocal immunofluorescencePhysiological roleIschemic hepatocytesCell typesBiochemical assaysSingle cellsLiver damageBACKGROUND/Western blotCAMP production
2004
Effects of increased intracellular cAMP on carbachol-stimulated zymogen activation, secretion, and injury in the pancreatic acinar cell
Chaudhuri A, Kolodecik TR, Gorelick FS. Effects of increased intracellular cAMP on carbachol-stimulated zymogen activation, secretion, and injury in the pancreatic acinar cell. AJP Gastrointestinal And Liver Physiology 2004, 288: g235-g243. PMID: 15458924, PMCID: PMC2975016, DOI: 10.1152/ajpgi.00334.2004.Peer-Reviewed Original ResearchConceptsPancreatic acinar cellsSecretion of amylaseAcinar cellsCell injuryIntracellular cAMPForms of pancreatitisParameters of injuryAcinar cell injuryCholinergic agonist carbacholEnzyme secretionRat pancreatic acinar cellsAcute pancreatitisZymogen activationAgonist carbacholSupraphysiological concentrationsCellular injuryCarbacholInjuryPancreatic aciniCAMP productionUnstimulated aciniSecretionCellular cAMPPhysiological concentrationsPancreatitis
1999
Modulation of a pacemaker current through Ca2+-induced stimulation of cAMP production
Lüthi A, McCormick D. Modulation of a pacemaker current through Ca2+-induced stimulation of cAMP production. Nature Neuroscience 1999, 2: 634-641. PMID: 10404196, DOI: 10.1038/10189.Peer-Reviewed Original ResearchConceptsHyperpolarization-activated cation currentIh activationThalamocortical rhythmsThalamocortical activityCation currentNeuronal propertiesPacemaker currentCAMP productionPersistent activationProlonged changesProlonged activationBrief increaseDependent modulationSlow recurrenceActivationSubsequent productionCa2RecurrenceModulation
1995
Stimulation of chloride transport by cAMP in rat proximal tubules
Wang T, Segal AS, Giebisch G, Aronson PS. Stimulation of chloride transport by cAMP in rat proximal tubules. American Journal Of Physiology 1995, 268: f204-f210. PMID: 7864157, DOI: 10.1152/ajprenal.1995.268.2.f204.Peer-Reviewed Original ResearchConceptsRat proximal tubuleProximal tubulesDibutyryl cAMPCapillary perfusateLate proximal tubuleProximal tubule cellsCl- channel blockerCl- channelsTranscellular Cl absorptionAddition of forskolinLuminal DIDSSensitive Cl- channelBasolateral membraneLuminal perfusateTubule cellsCAMP productionFluid absorptionCl absorptionMM ClPerfusateJclRecent evidenceLuminal membraneTubulesEffect of cAMP
1994
Angiotensin II stimulation of Na-H antiporter activity is cAMP independent in OKP cells
Cano A, Miller RT, Alpern RJ, Preisig PA. Angiotensin II stimulation of Na-H antiporter activity is cAMP independent in OKP cells. American Journal Of Physiology 1994, 266: c1603-c1608. PMID: 8023891, DOI: 10.1152/ajpcell.1994.266.6.c1603.Peer-Reviewed Original ResearchConceptsPertussis toxin-sensitive G proteinToxin-sensitive G proteinNa-H antiporter activityAngiotensin IINa-H antiporterOKP cellsAngiotensin II concentrationAngiotensin II receptorsAbsence of IBMXAngiotensin II stimulationG proteinsCyclic monophosphateProximal tubule apical membraneOpossum kidney cellsConcentration-dependent mannerCAMP-independent mechanismM losartanAcute treatmentAT1 receptorII stimulationPertussis toxinII receptorsProximal tubulesCAMP productionStimulatory effect
1993
Platelet serotonin studies in hyperserotonemic relatives of children with autistic disorder
Cook E, Arora R, Anderson G, Berry-Kravis E, Yan S, Yeoh HC, Sklena P, Charak D, Leventhal B. Platelet serotonin studies in hyperserotonemic relatives of children with autistic disorder. Life Sciences 1993, 52: 2005-2015. PMID: 7684805, DOI: 10.1016/0024-3205(93)90685-v.Peer-Reviewed Original ResearchConceptsLysergic acid diethylamideParoxetine bindingWhole bloodSubgroup of subjectsHyperserotonemia of autismProstaglandin E1Arginine vasopressinDensity of plateletsCAMP productionAcid diethylamideLSD bindingAutistic disorderSignificant differencesSignificant correlationBloodSubjectsBasalSubgroupsPlateletsChildrenAutistic probandsUptake VmaxAutistic childrenRecent Advances in the Molecular Biology of Growth-Hormone Secreting Human Pituitary Tumours
Adams E, Buchfelder M, Hüttner A, Moreth S, Fahlbusch R. Recent Advances in the Molecular Biology of Growth-Hormone Secreting Human Pituitary Tumours. Experimental And Clinical Endocrinology & Diabetes 1993, 101: 12-16. PMID: 8477819, DOI: 10.1055/s-0029-1211202.Peer-Reviewed Original ResearchConceptsGH geneX-chromosome inactivationAlpha s subunitGene expressionPromoter regionS subunitsSequence analysisMolecular biologyRFLP analysisDNA technologyGs proteinGenesGH gene expressionSingle cellsSomatic mutationsHuman pituitary tumorsMutationsCAMP productionGsp mutationsExpressionCyclase activityRecent advancesSubunitsBiologyAdenyl cyclase activity
1986
Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
Coca-Prados M, Wax M. Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8754-8758. PMID: 3022303, PMCID: PMC387010, DOI: 10.1073/pnas.83.22.8754.Peer-Reviewed Original ResearchConceptsSimian virus 40Ciliary epithelial cellsSouthern blot analysisLarge tumor antigenHuman ciliary epithelial cellsEpithelial cellsFunctional conservationProtein phosphorylationDirect bindingCell DNAParent cellsBlot analysisCell proliferationCellular cAMP productionReceptor-mediated processEpithelial cell DNAViral DNAImmunoprecipitationDNACAMP productionSelective drugsCell clonesMembrane preparationsCellsReceptors
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