2012
Cardiovascular dysregulation of miR‐17‐92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis
Danielson LS, Park DS, Rotllan N, Chamorro‐Jorganes A, Guijarro MV, Fernandez‐Hernando C, Fishman GI, Phoon CK, Hernando E. Cardiovascular dysregulation of miR‐17‐92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. The FASEB Journal 2012, 27: 1460-1467. PMID: 23271053, PMCID: PMC3606524, DOI: 10.1096/fj.12-221994.Peer-Reviewed Original ResearchConceptsSmooth muscle tissueHypertrophic cardiomyopathyMiR-17Dose-dependent inductionMuscle tissueCardiovascular dysregulationArrhythmia inducibilityNovel direct targetMicroRNA cluster miR-17Lethal cardiomyopathyPremature mortalityTransgenic heartsMouse modelHeart sizeCluster miR-17CardiomyopathyPrecise mechanismLuciferase assayDirect targetExpression levelsPathological functionsExpression analysisConditional overexpressionTransgenic animalsHeart
2003
Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium
Rauz S, Walker EA, Hughes SV, Coca-Prados M, Hewison M, Murray PI, Stewart PM. Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium. Investigative Ophthalmology & Visual Science 2003, 44: 1643-1651. PMID: 12657604, DOI: 10.1167/iovs.02-0514.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneBlotting, NorthernCell LineCiliary BodyDexamethasoneDose-Response Relationship, DrugDrug CombinationsEpithelial Sodium ChannelsGene Expression RegulationGlucocorticoidsHormone AntagonistsHumansImmediate-Early ProteinsIn Situ HybridizationIsoenzymesMifepristoneMineralocorticoid Receptor AntagonistsNuclear ProteinsPigment Epithelium of EyeProtein Serine-Threonine KinasesReceptors, GlucocorticoidReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodium ChannelsSpironolactoneConceptsExpression of SGK1Human ocular ciliary epitheliumODM-2 cellsOcular ciliary epitheliumSodium transportCiliary epitheliumENaC subunitsNPE cell linesEpithelial sodium channel (ENaC) subunitsAqueous humor productionSodium channel subunitsExpression of ENaCRadioligand-binding assaysDose-dependent inductionIsoform 1Epithelial sodium channelCorticosteroid regulationHuman ocularAldosteroneCiliary epithelial bilayerRU26752DexamethasoneRT-PCR analysisSodium channelsChannel subunits
1995
Regulation of proliferation and production of prostate-specific antigen in androgen-sensitive prostatic cancer cells, LNCaP, by dihydrotestosterone
Lee C, Sutkowski D, Sensibar J, Zelner D, Kim I, Amsel I, Shaw N, Prins G, Kozlowski J. Regulation of proliferation and production of prostate-specific antigen in androgen-sensitive prostatic cancer cells, LNCaP, by dihydrotestosterone. Endocrinology 1995, 136: 796-803. DOI: 10.1210/en.136.2.796.Peer-Reviewed Original ResearchProstate-specific antigenProduction of prostate-specific antigenProstate-specific antigen secretionConcentrations of dihydrotestosteroneEffects of androgensDihydrotestosterone concentrationsAndrogen receptorAndrogen-sensitive human prostate cancer cell lineAndrogen-sensitive prostate cancer cellsIncreasing concentrations of DHTLow concentrations of dihydrotestosteroneCharcoal-stripped fetal bovine serumAnalysis of androgen receptorHuman prostate cancer cell linesProstate cancer cell linesCellular proliferationProstate cancer cellsNuclear androgen receptorAmount of receptor proteinDose-dependent inductionProportion of cellsFetal bovine serumCancer cell linesLNCaP cellsRegulation of proliferationRegulation of proliferation and production of prostate-specific antigen in androgen-sensitive prostatic cancer cells, LNCaP, by dihydrotestosterone
Lee C, Sutkowski D, Sensibar J, Zelner D, Kim I, Amsel I, Shaw N, Prins G, Kozlowski J. Regulation of proliferation and production of prostate-specific antigen in androgen-sensitive prostatic cancer cells, LNCaP, by dihydrotestosterone. Endocrinology 1995, 136: 796-803. PMID: 7530653, DOI: 10.1210/endo.136.2.7530653.Peer-Reviewed Original ResearchConceptsHigher DHT concentrationsEffects of androgensConcentrations of dihydrotestosteroneDHT concentrationsPSA secretionAndrogen receptorCharcoal-stripped fetal bovine serumProstate-specific antigen (PSA) productionHuman prostatic cancer cell linesLow DHT concentrationsProstatic cancer cell linesDose-related mannerNuclear androgen receptorProstate-specific antigenCellular proliferationProstatic cancer cellsProduction of PSADose-dependent inductionWestern blot analysisCancer cell linesProportion of cellsDHT levelsLNCaP cellsM dihydrotestosteronePositive staining
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