2025
Prevention and treatment of noise-induced hearing loss and cochlear synapse degeneration by potassium channel blockers in vivo
Zhao H, Liu L, Lu X, Quinonez A, Roberts R, Zhai T, Liang C. Prevention and treatment of noise-induced hearing loss and cochlear synapse degeneration by potassium channel blockers in vivo. Hearing Research 2025, 464: 109319. PMID: 40450915, PMCID: PMC12318480, DOI: 10.1016/j.heares.2025.109319.Peer-Reviewed Original ResearchNoise-induced hearing lossDistortion product otoacoustic emissionsAuditory brainstem responseNoise exposureHearing lossAcoustic startle responseTreatment of noise-induced hearing lossHidden hearing lossSystemic administrationInduce hearing lossGAL-021Active cochlear mechanicsChannel blockersOtoacoustic emissionsBrainstem responseHearing disordersAmeliorating noise-induced hearing lossCochlear mechanicsPost-exposure administrationSynapse degenerationInner hair cellsBK channelsAdministration timeIntraperitoneal injectionPharmacological treatmentBK channels mediate a presynaptic form of mGluR-LTD in the neonatal hippocampus
Ancatén-González C, Meza R, Gonzalez-Sanabria N, Segura I, Alcaino A, Peña-Pichicoi A, Latorre R, Chiu C, Chávez A. BK channels mediate a presynaptic form of mGluR-LTD in the neonatal hippocampus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2025, 122: e2411506122. PMID: 39773031, PMCID: PMC11745352, DOI: 10.1073/pnas.2411506122.Peer-Reviewed Original ResearchConceptsBK channelsLong-term depressionMGluR-LTDNeonatal hippocampusPostsynaptic metabotropic glutamate receptorsActivation of BK channelsChannel open probabilityCA3-CA1 synapsesMetabotropic glutamate receptorsArachidonic acid metabolitesRegulating synaptic strengthActivity-dependent changesOpen probabilityHippocampal slicesCircuit maturationCA3-CA1Glutamate receptorsElectrophysiological propertiesPresynaptic formP7-P10Synaptic strengthAcid metabolitesCentral synapsesNeuronal functionSynaptic function
2021
Excess extracellular K+ causes inner hair cell ribbon synapse degeneration
Zhao H, Zhu Y, Liu L. Excess extracellular K+ causes inner hair cell ribbon synapse degeneration. Communications Biology 2021, 4: 24. PMID: 33398038, PMCID: PMC7782724, DOI: 10.1038/s42003-020-01532-w.Peer-Reviewed Original ResearchConceptsCochlear synaptopathySynapse degenerationRibbon synapsesNoise exposureNoise-induced cochlear synaptopathyInner hair cell ribbon synapsesIHC ribbon synapsesGlutamate receptor agonistsBK channel blockersHair cell ribbon synapsesReceptor agonistFirst synapseChannel blockersHearing lossIHC ribbonsHearing disordersDegenerationBK channelsPotential targetSynaptopathyAuditory systemSynapsesExposureSwellingExcitotoxicity
2020
Calcium‐induced calcium release in proximity to hair cell BK channels revealed by PKA activation
Bai J, Xue N, Lawal O, Nyati A, Santos‐Sacchi J, Navaratnam D. Calcium‐induced calcium release in proximity to hair cell BK channels revealed by PKA activation. Physiological Reports 2020, 8: e14449. PMID: 32748549, PMCID: PMC7399380, DOI: 10.14814/phy2.14449.Peer-Reviewed Original ResearchConceptsBK channelsBK currentsCalcium-activated potassium channelsLarge-conductance calcium-activated potassium channelsCalcium-induced calcium releaseEntry of CaSlo subunitSynaptic releaseCalcium releaseChicken hair cellsPotassium channelsHair cellsIP3 receptorCICRA ActivationPKA activationActivationCritical roleImagingReleaseRyanodineSex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney
Kim J, Song K, Lee E, Xu S, Wang T. Sex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04872.Peer-Reviewed Original ResearchMaxi-K channelsKO miceKlotho expressionMouse kidneyBartter's syndromeWT miceMaxi-KFemale WTRenal outer medullary potassium channelSurvival rateWT mouse kidneysUrinary potassium excretionProtein levelsBK channelsFemale mouse kidneyPotassium excretionVolume depletionROMKElectrolyte imbalancePotassium channelsBK expressionII phenotypeLife-threateningSex differencesBlood pressure
2018
Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells
Lu M, Li JR, Alvarez-Lugo L, Li Y, Yu S, Li X, Shi B, Chai TC. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. American Journal Of Physiology - Cell Physiology 2018, 314: c643-c653. PMID: 29466671, DOI: 10.1152/ajpcell.00339.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic AMP-Dependent Protein KinasesFemaleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLipopolysaccharide ReceptorsLipopolysaccharidesLymphocyte Antigen 96Membrane PotentialsMice, Inbred C57BLPatch-Clamp TechniquesPotassiumSignal TransductionToll-Like Receptor 4Urinary BladderUrinary Tract InfectionsUrotheliumConceptsBK channel activityBK channelsChannel activityUmbrella cellsUrinary tract infectionFemale C57BL6 micePotassium channel inhibitorsUropathogenic Escherichia coliProtein kinase ATract infectionsLPS effectsC57BL6 miceBladder urotheliumLamina propriaMin of exposureChannel inhibitorsBacterial infectionsLPS receptorUrothelial tissuesUrothelial cellsUrotheliumLipopolysaccharidePotassium channelsRT-PCRPKA blocker
2010
β4-Subunit increases Slo responsiveness to physiological Ca2+ concentrations and together with β1 reduces surface expression of Slo in hair cells
Bai JP, Surguchev A, Navaratnam D. β4-Subunit increases Slo responsiveness to physiological Ca2+ concentrations and together with β1 reduces surface expression of Slo in hair cells. American Journal Of Physiology - Cell Physiology 2010, 300: c435-c446. PMID: 21178105, PMCID: PMC3063969, DOI: 10.1152/ajpcell.00449.2010.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium SignalingCell MembraneChickensCochleaDown-RegulationHair Cells, AuditoryIon Channel GatingLarge-Conductance Calcium-Activated Potassium Channel beta SubunitsLarge-Conductance Calcium-Activated Potassium ChannelsMechanotransduction, CellularMembrane PotentialsOocytesXenopus laevisConceptsLow-frequency hair cellsHair cellsLarge-conductance potassium channelsBK channelsSurface expressionBK channel functionNonmammalian vertebratesAccessory subunitsΑ-subunitMolecular underpinningsΒ-subunitChannel functionSubunitsΒ4 subunitPhysiological Ca2Critical rolePotassium channelsCellsBK currentsAdditional mechanismExpressionVertebratesDramatic increaseChicksIntracellular
2009
Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy
Wang L, Sigworth FJ. Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 2009, 461: 292-295. PMID: 19718020, PMCID: PMC2797367, DOI: 10.1038/nature08291.Peer-Reviewed Original ResearchConceptsVoltage sensor domainNative membrane environmentMembrane proteinsMembrane environmentChannel voltage-sensor domainElectron cryomicroscopyVoltage-activated potassium channelsSingle-particle electron cryomicroscopyIndividual protein particlesPotassium channelsBK potassium channelsLarge conductance calciumLipid membranesX-ray crystal structureBK channelsStructural studiesProtein particlesProteinCryomicroscopyCrystal structureFormation of crystalsDomainStructureCrystalsChannels
2007
Four-turn α-Helical Segment Prevents Surface Expression of the Auxiliary hβ2 Subunit of BK-type Channel*
Lv C, Chen M, Gan G, Wang L, Xu T, Ding J. Four-turn α-Helical Segment Prevents Surface Expression of the Auxiliary hβ2 Subunit of BK-type Channel*. Journal Of Biological Chemistry 2007, 283: 2709-2715. PMID: 17991741, DOI: 10.1074/jbc.m704440200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineEndoplasmic ReticulumGene ExpressionHumansLarge-Conductance Calcium-Activated Potassium ChannelsMicroscopy, FluorescenceModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedPatch-Clamp TechniquesProtein Structure, SecondaryProtein SubunitsRatsRecombinant Fusion ProteinsSequence Homology, Amino AcidConceptsBK-type channelsSurface expressionRat chromaffin cellsExtracellular loopPancreatic beta cellsTrafficking mechanismsN-terminusDRG neuronsHelical segmentsToxin sensitivityBeta cellsClamp techniqueHelp of immunofluorescenceAuxiliary beta subunitsAlpha-helical segmentsChromaffin cellsBeta2 subunitBK channelsHbeta2Retention signalChannel regulatorRegulatory mechanismsBeta subunitEndoplasmic reticulumLarge conductanceHypoxia increases BK channel activity in the inner mitochondrial membrane
Gu XQ, Siemen D, Parvez S, Cheng Y, Xue J, Zhou D, Sun X, Jonas EA, Haddad GG. Hypoxia increases BK channel activity in the inner mitochondrial membrane. Biochemical And Biophysical Research Communications 2007, 358: 311-316. PMID: 17481584, DOI: 10.1016/j.bbrc.2007.04.110.Peer-Reviewed Original Research
2001
Ca2+-binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca2+-dependent activation
Bian S, Favre I, Moczydlowski E. Ca2+-binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca2+-dependent activation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 4776-4781. PMID: 11274367, PMCID: PMC31910, DOI: 10.1073/pnas.081072398.Peer-Reviewed Original ResearchConceptsIntracellular COOH-terminal domainDependent activationBK channelsCOOH-terminal domainProtein blot assayBiochemical approachesStructural basisBiophysical analysisBK proteinAsp residuesControl proteinHEK293 cellsEscherichia coliProteinElectrophysiological assaysBlot assaysLarge conductanceTerminal fragmentSoluble formActivationResiduesFunctional correlationSpecific bindingSpecific regionsBinding
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