2020
Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF
Stewart E, Wang X, Chupp GL, Montgomery RR. Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF. Journal Of Leukocyte Biology 2020, 108: 1555-1564. PMID: 32911570, PMCID: PMC8087109, DOI: 10.1002/jlb.5ma0720-770rr.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAsthmaFemaleHumansLymphocytesMacrophagesMaleMiddle AgedNeutrophilsSingle-Cell AnalysisSputumConceptsAirway immune cellsSource of inflammationChronic inflammatory diseaseInflammatory cell typesStudy of pathogenesisMultiple distinct subtypesHeterogeneity of diseaseBronchial epithelial cellsAirway inflammationCell typesAsthmatic patientsClinical responseEosinophilic infiltrateAnalysis of sputumAsthmatic inflammationFunctional statusClinical managementImmune cellsInflammatory diseasesHealthy controlsInflammatory responseAirway cellsSputumDistinct subtypesInflammation
2017
Multiparameter Single Cell Profiling of Airway Inflammatory Cells
Yao Y, Welp T, Liu Q, Niu N, Wang X, Britto CJ, Krishnaswamy S, Chupp G, Montgomery RR. Multiparameter Single Cell Profiling of Airway Inflammatory Cells. Cytometry Part B Clinical Cytometry 2017, 92: 12-20. PMID: 27807928, PMCID: PMC5250532, DOI: 10.1002/cyto.b.21491.Peer-Reviewed Original ResearchConceptsSingle-cell methodsComplex trait diseasesSubgroup of asthmaticsSingle-cell analysisMultiparameter single cell analysisMillions of patientsTranscriptional analysisImmunologic underpinningsInduced sputumAirway diseaseAsthmatic patientsAirway samplesCell subsetsImmune statusFunctional statusClinical severityDistinct biologic mechanismsTreatment successPhysiologic manifestationsBiologic mechanismsCystic fibrosisCellular analysisPatientsCytometry studiesU.S. population
2014
Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging
Qian F, Guo X, Wang X, Yuan X, Chen S, Malawista SE, Bockenstedt LK, Allore HG, Montgomery RR. Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging. Aging 2014, 6: 131-139. PMID: 24595889, PMCID: PMC3969281, DOI: 10.18632/aging.100642.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAgingEnergy MetabolismFemaleHumansMaleNeutrophilsToll-Like Receptor 1Young AdultConceptsToll-like receptor 1Polymorphonuclear leukocytesOlder adultsChemokine IL-8Loss of CD62LAge-dependent deficienciesPotential therapeutic opportunitiesHuman polymorphonuclear leukocytesTLR1 expressionIL-8PMN functionPMN activationMultiple pathwaysImmune functionYoung donorsP38 MAP kinaseReceptor 1Bacterial infectionsTherapeutic opportunitiesProgressive declineGlucose uptakeReduced expressionAdultsStimulationLeukocytes
2009
Inhibition of Neutrophil Function by Two Tick Salivary Proteins
Guo X, Booth CJ, Paley MA, Wang X, DePonte K, Fikrig E, Narasimhan S, Montgomery RR. Inhibition of Neutrophil Function by Two Tick Salivary Proteins. Infection And Immunity 2009, 77: 2320-2329. PMID: 19332533, PMCID: PMC2687334, DOI: 10.1128/iai.01507-08.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesPMN functionNumber of PMNPMN integrinsPMN adherenceNeutrophil functionSpirochete burdenTick salivary proteinsTick salivaLyme diseaseTick attachmentSalivary glandsBorrelia burgdorferiTick feedingCausative agentReduced levelsInhibitory proteinSalivaBlood mealAntihemostatic activityInfectionInhibitionSalivary proteinsHematophagous arthropodsTick Ixodes scapularis
2006
Human Neutrophil Calprotectin Reduces the Susceptibility of Borrelia burgdorferi to Penicillin
Montgomery RR, Schreck K, Wang X, Malawista SE. Human Neutrophil Calprotectin Reduces the Susceptibility of Borrelia burgdorferi to Penicillin. Infection And Immunity 2006, 74: 2468-2472. PMID: 16552081, PMCID: PMC1418918, DOI: 10.1128/iai.74.4.2468-2472.2006.Peer-Reviewed Original ResearchConceptsClearance of spirochetesB. burgdorferiSites of inflammationTherapeutic dosesCalprotectinGrowth of spirochetesLeukocyte componentsNeutrophil calprotectinAntibiotic sensitivitySpirochetal agentLyme diseaseTherapeutic antibioticsBorrelia burgdorferiPenicillin GProtein calprotectinInflammationBurgdorferiSpirochetesDiseaseDoxycyclineDosesClearance