2023
Integrated Analysis of Tracheobronchial Fluid from Before and After Cardiopulmonary Bypass Reveals Activation of the Integrated Stress Response and Altered Pulmonary Microvascular Permeability
Habet V, Li N, Qi J, Peng G, Charkoftaki G, Vasiliou V, Sharma L, Pober J, Dela Cruz C, Yan X, Pierce R. Integrated Analysis of Tracheobronchial Fluid from Before and After Cardiopulmonary Bypass Reveals Activation of the Integrated Stress Response and Altered Pulmonary Microvascular Permeability. The Yale Journal Of Biology And Medicine 2023, 96: 23-42. PMID: 37009190, PMCID: PMC10052603, DOI: 10.59249/kfyz8002.Peer-Reviewed Original ResearchMeSH KeywordsCapillary PermeabilityCardiopulmonary BypassChildChild, PreschoolEndothelial CellsHumansLungProteomicsConceptsIntegrated stress responseProteomic analysisTranscriptional activityStress responseSingle-cell RNA sequencingCell RNA sequencingHuman pulmonary microvascular endothelial cellsMulti-omics approachCell type annotationRespiratory transport chainUnbiased proteomic analysisUpregulation of proteinsIngenuity Pathway AnalysisCardiopulmonary bypassCell clusteringProtective cellular responseFunctional cellular assaysDistinct cell populationsDEG analysisCellular phenotypesRNA sequencingPathway analysisTransport chainCellular responsesImmune cells
2021
Immune dysregulation and autoreactivity correlate with disease severity in SARS-CoV-2-associated multisystem inflammatory syndrome in children
Ramaswamy A, Brodsky NN, Sumida TS, Comi M, Asashima H, Hoehn KB, Li N, Liu Y, Shah A, Ravindra NG, Bishai J, Khan A, Lau W, Sellers B, Bansal N, Guerrerio P, Unterman A, Habet V, Rice AJ, Catanzaro J, Chandnani H, Lopez M, Kaminski N, Dela Cruz CS, Tsang JS, Wang Z, Yan X, Kleinstein SH, van Dijk D, Pierce RW, Hafler DA, Lucas CL. Immune dysregulation and autoreactivity correlate with disease severity in SARS-CoV-2-associated multisystem inflammatory syndrome in children. Immunity 2021, 54: 1083-1095.e7. PMID: 33891889, PMCID: PMC8043654, DOI: 10.1016/j.immuni.2021.04.003.Peer-Reviewed Original ResearchConceptsMIS-C patientsDisease severityInflammatory syndromeTCR repertoireSARS-CoV-2-associated multisystem inflammatory syndromeAsymptomatic SARS-CoV-2 infectionSARS-CoV-2 infectionAdult COVID-19Post-infectious complicationsMultisystem inflammatory syndromeCytotoxicity genesHealthy pediatricImmune dysregulationMemory TActive infectionMyeloid dysfunctionPatientsSingle-cell RNA sequencingFlow cytometrySerum proteomicsRepertoire analysisElevated expressionSeverityAlarminsCOVID-19
2018
Sera From Children After Cardiopulmonary Bypass Reduces Permeability of Capillary Endothelial Cell Barriers
Pierce RW, Zahr RA, Kandil S, Faustino EVS, Pober JS. Sera From Children After Cardiopulmonary Bypass Reduces Permeability of Capillary Endothelial Cell Barriers. Pediatric Critical Care Medicine 2018, 19: 609-618. PMID: 29652749, PMCID: PMC6037548, DOI: 10.1097/pcc.0000000000001553.Peer-Reviewed Original ResearchConceptsTransendothelial electrical resistanceCardiopulmonary bypassCapillary endothelial cellsEndothelial cellsCapillary leakPatient seraClaudin-5Pulmonary capillary endothelial cellsBarrier functionPulmonary microvascular endothelial cellsReal-time quantitative reverse transcription polymerase chain reactionTertiary pediatric hospitalQuantitative reverse transcription polymerase chain reactionCardiopulmonary bypass circuitCongenital heart diseaseReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionMicrovascular endothelial cellsEndothelial cell barrierCohort studyInflammatory mediatorsPediatric hospitalUnclear etiologyBarrier disruptionHeart disease
2016
Association of thrombophilia and catheter‐associated thrombosis in children: a systematic review and meta‐analysis
Neshat‐Vahid S, Pierce R, Hersey D, Raffini LJ, Faustino EV. Association of thrombophilia and catheter‐associated thrombosis in children: a systematic review and meta‐analysis. Journal Of Thrombosis And Haemostasis 2016, 14: 1749-1758. PMID: 27306795, PMCID: PMC5035642, DOI: 10.1111/jth.13388.Peer-Reviewed Original ResearchConceptsDeep vein thrombosisCatheter-associated thrombosisCentral venous catheter useElevated factor VIII levelsAssociation of thrombophiliaRoutine thrombophilia testingVenous catheter useCochrane Central RegisterPediatric risk factorsPooled prevalence rateFactor VIII levelsProtein C deficiencyRandom-effects modelWeb of ScienceThrombophilic traitsCatheter useCentral RegisterCohort studyFV Leiden mutationVein thrombosisControlled TrialsVenous thrombosisPediatric studiesRisk factorsOdds ratio
2014
Use of procalcitonin for the prediction and treatment of acute bacterial infection in children
Pierce R, Bigham MT, Giuliano JS. Use of procalcitonin for the prediction and treatment of acute bacterial infection in children. Current Opinion In Pediatrics 2014, 26: 292-298. PMID: 24739491, DOI: 10.1097/mop.0000000000000092.Peer-Reviewed Original ResearchConceptsAntibiotic therapySerum procalcitoninTreatment failureNoninfectious systemic inflammatory response syndromeBacterial infectionsSystemic inflammatory response syndromeSerial PCT measurementsDuration of therapyCentral venous cathetersInflammatory response syndromeAcute bacterial infectionUse of procalcitoninC-reactive proteinInvasive bacterial infectionsPresence of infectionReliable serum markerSickle cell diseaseNoninfectious inflammationPCT levelsVenous cathetersResponse syndromeBacterial sepsisSerum markersPrognostic utilityPatient population