2020
Hypothalamic oestrogen receptor alpha establishes a sexually dimorphic regulatory node of energy expenditure
van Veen JE, Kammel LG, Bunda PC, Shum M, Reid MS, Massa MG, Arneson D, Park JW, Zhang Z, Joseph AM, Hrncir H, Liesa M, Arnold AP, Yang X, Correa SM. Hypothalamic oestrogen receptor alpha establishes a sexually dimorphic regulatory node of energy expenditure. Nature Metabolism 2020, 2: 351-363. PMID: 32377634, PMCID: PMC7202561, DOI: 10.1038/s42255-020-0189-6.Peer-Reviewed Original ResearchConceptsFemale-biased expressionMale-biased expressionSex-biased expressionSingle-cell RNA transcriptomicsPrecise neuronal populationsEstrogen receptor alphaReceptor alphaGene functionVentromedial hypothalamusRegulatory nodesRNA transcriptomicsMale developmentNeuronal populationsMouse ventromedial hypothalamusSitu hybridizationEnergy homeostasisEnergy expenditureCore temperatureExpressionTachykinin-1RPRMReduced core temperatureVMH neuronsChemogenetic activationPhysical activity
2018
Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice
Michael HT, Graff-Cherry C, Chin S, Rauck C, Habtemichael AD, Bunda P, Smith T, Campos MM, Bharti K, Arnheiter H, Merlino G, Day CP. Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice. Investigative Ophthalmology & Visual Science 2018, 59: 6067-6073. PMID: 30590377, PMCID: PMC6314104, DOI: 10.1167/iovs.18-25186.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternChoroidEctopic Gene ExpressionEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenotyping TechniquesImmunohistochemistryIntramolecular OxidoreductasesIrisMaleMelanocytesMiceMice, Inbred C57BLMice, TransgenicMicrophthalmia-Associated Transcription FactorMicrophthalmosRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionSkinConceptsMicrophthalmia transcription factorMITF-M expressionEctopic expressionEpithelium gene expressionRetinal dysplasiaNew mouse lineMouse eyeRetinal structureCutaneous melanocytesRPE structureUveal melanocytesRPE functionMiceMouse linesComplete deficiencyPigmentary genesInducible ectopic expressionMelanocytesMITF isoformsIris melanocytesMicrophthalmiaPhenotypic abnormalitiesEyesMITFPartial rescue