2019
Hsp110 mitigates α-synuclein pathology in vivo
Taguchi YV, Gorenberg EL, Nagy M, Thrasher D, Fenton WA, Volpicelli-Daley L, Horwich AL, Chandra SS. Hsp110 mitigates α-synuclein pathology in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 24310-24316. PMID: 31685606, PMCID: PMC6883785, DOI: 10.1073/pnas.1903268116.Peer-Reviewed Original ResearchConceptsΑ-synuclein pathologyOverexpression of Hsp110Α-synuclein aggregationPresynaptic protein α-synucleinΑ-synuclein seedsΑ-synuclein oligomersLewy bodiesMouse modelParkinson's diseaseCell culture modelSynaptic proteomeΑ-synucleinProtein α-synucleinPathologyCulture modelDiseaseMammalian cell culture modelsProtein changesOverexpressionVivoHsp110Molecular facilitatorsMiceUnbiased analysisBrain
2014
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
Bandyopadhyay U, Fenton WA, Horwich AL, Nagy M. Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection. Journal Of Visualized Experiments 2014, 51168. PMID: 24457537, PMCID: PMC4089401, DOI: 10.3791/51168.Peer-Reviewed Original ResearchConceptsLaser capture microdissectionMotor neuronsSpinal cordMotor neuron cell bodiesCapture microdissectionMouse spinal cordNeuron cell bodiesTotal cell populationCell typesPharmacologic treatmentStained neuronsCell bodiesNeurodegenerative diseasesNeuronsExogenous RNasesQRT-PCRSame cell typeCell populationsCordRNA damageDiseaseTotal RNASuch preparationsMicrodissectionTranscriptional differences