2023
Membrane remodeling properties of the Parkinson’s disease protein LRRK2
Wang X, Espadas J, Wu Y, Cai S, Ge J, Shao L, Roux A, De Camilli P. Membrane remodeling properties of the Parkinson’s disease protein LRRK2. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309698120. PMID: 37844218, PMCID: PMC10614619, DOI: 10.1073/pnas.2309698120.Peer-Reviewed Original ResearchCell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original ResearchThree-dimensional structured illumination microscopy with enhanced axial resolution
Li X, Wu Y, Su Y, Rey-Suarez I, Matthaeus C, Updegrove T, Wei Z, Zhang L, Sasaki H, Li Y, Guo M, Giannini J, Vishwasrao H, Chen J, Lee S, Shao L, Liu H, Ramamurthi K, Taraska J, Upadhyaya A, La Riviere P, Shroff H. Three-dimensional structured illumination microscopy with enhanced axial resolution. Nature Biotechnology 2023, 41: 1307-1319. PMID: 36702897, PMCID: PMC10497409, DOI: 10.1038/s41587-022-01651-1.Peer-Reviewed Original Research
2022
Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
Yang S, Park D, Manning L, Hill SE, Cao M, Xuan Z, Gonzalez I, Dong Y, Clark B, Shao L, Okeke I, Almoril-Porras A, Bai J, De Camilli P, Colón-Ramos DA. Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9. Neuron 2022, 110: 824-840.e10. PMID: 35065714, PMCID: PMC9017068, DOI: 10.1016/j.neuron.2021.12.031.Peer-Reviewed Original ResearchConceptsSynaptic vesicle cycleVesicle cyclePresynaptic autophagyAutophagosome biogenesisATG-9Only transmembrane proteinTrans-Golgi networkCellular degradation pathwayPresynaptic sitesActivity-dependent mannerTransmembrane proteinSynaptojanin 1Synaptic fociBiogenesisAutophagyNeuronal healthDegradation pathwayTraffickingPathwayParkinson's diseaseSynaptic activityNeuronal activityElegansSitesEndocytosis
2021
Differential adhesion regulates neurite placement via a retrograde zippering mechanism
Sengupta T, Koonce NL, Vázquez-Martínez N, Moyle MW, Duncan LH, Emerson SE, Han X, Shao L, Wu Y, Santella A, Fan L, Bao Z, Mohler W, Shroff H, Colón-Ramos DA. Differential adhesion regulates neurite placement via a retrograde zippering mechanism. ELife 2021, 10: e71171. PMID: 34783657, PMCID: PMC8843091, DOI: 10.7554/elife.71171.Peer-Reviewed Original ResearchConceptsDifferential adhesionDifferential adhesion mechanismsSYG-1SYG-2Developmental programEmbryonic developmentNeurite tipsZippering mechanismBiophysical principlesNeurite shaftSynaptic specificityBrain neuropilSingle neuriteLayers occursAlternate mechanismAdhesion mechanismExpressionNeuritesZipperingAdhesionMechanismOutgrowthStructural and developmental principles of neuropil assembly in C. elegans
Moyle MW, Barnes KM, Kuchroo M, Gonopolskiy A, Duncan LH, Sengupta T, Shao L, Guo M, Santella A, Christensen R, Kumar A, Wu Y, Moon KR, Wolf G, Krishnaswamy S, Bao Z, Shroff H, Mohler WA, Colón-Ramos DA. Structural and developmental principles of neuropil assembly in C. elegans. Nature 2021, 591: 99-104. PMID: 33627875, PMCID: PMC8385650, DOI: 10.1038/s41586-020-03169-5.Peer-Reviewed Original ResearchConceptsSpecific sensory organsNerve ringCaenorhabditis elegansC. elegansMuscle quadrantsNeuropil organizationDevelopmental principlesTissue organizationSensory organsBehavioral circuitsElegansPioneer neuronsCell positionDevelopmental sequenceStratified architectureTemporal progressionPrecise circuitsPacked neuronsUnique morphologyNeuronsSequenceOutgrowthAssemblyHierarchical developmentNeuropil
2020
Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella
Chen M, Sun H, Boot M, Shao L, Chang SJ, Wang W, Lam TT, Lara-Tejero M, Rego EH, Galán JE. Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella. Science 2020, 369: 450-455. PMID: 32703879, PMCID: PMC8020367, DOI: 10.1126/science.aaz1333.Peer-Reviewed Original ResearchTorsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2
Rampello AJ, Laudermilch E, Vishnoi N, Prophet SM, Shao L, Zhao C, Lusk CP, Schlieker C. Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2. Journal Of Cell Biology 2020, 219: e201910185. PMID: 32342107, PMCID: PMC7265317, DOI: 10.1083/jcb.201910185.Peer-Reviewed Original ResearchConceptsNuclear pore biogenesisMyeloid leukemia factor 2FG nucleoporinsNuclear pore complex biogenesisNuclear envelope herniationsNuclear envelope reformationLive cell imaging platformProteomics-based approachNuclear envelope blebbingComplex biogenesisBleb formationUbiquitin conjugationNuclear envelopeATPase deficiencyBiogenesisHallmark phenotypePhenotypic hallmarksPOM121Factor 2Late markersLuminal componentsNup358CellsUbiquitinBlebbing
2019
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
Duncan L, Moyle M, Shao L, Sengupta T, Ikegami R, Kumar A, Guo M, Christensen R, Santella A, Bao Z, Shroff H, Mohler W, Colón-Ramos D. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. Journal Of Visualized Experiments 2019 DOI: 10.3791/59533-v.Peer-Reviewed Original ResearchIsotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution.
Duncan LH, Moyle MW, Shao L, Sengupta T, Ikegami R, Kumar A, Guo M, Christensen R, Santella A, Bao Z, Shroff H, Mohler W, Colón-Ramos DA. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. Journal Of Visualized Experiments 2019 PMID: 31233035, PMCID: PMC7255390, DOI: 10.3791/59533.Peer-Reviewed Original ResearchConceptsGene expressionC. elegans embryosCell lineage identityCell lineage analysisSingle-cell resolutionElegans embryosCaenorhabditis elegansC. elegansSelective plane illumination microscopyLight-sheet microscopyLineage analysisNematode embryosCell resolutionEntire nervous systemSubcellular levelOnly organismSubcellular resolutionElegansCellular originSingle cellsEmbryosIllumination microscopyEmbryogenesisMorphological dynamicsExpression
2017
Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse.
Ashdown GW, Burn GL, Williamson DJ, Pandžić E, Peters R, Holden M, Ewers H, Shao L, Wiseman PW, Owen DM. Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Biophysical Journal 2017, 112: 1703-1713. PMID: 28445761, PMCID: PMC5406376, DOI: 10.1016/j.bpj.2017.01.038.Peer-Reviewed Original ResearchActin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
Beach JR, Bruun KS, Shao L, Li D, Swider Z, Remmert K, Zhang Y, Conti MA, Adelstein RS, Rusan NM, Betzig E, Hammer JA. Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments. Nature Cell Biology 2017, 19: 85-93. PMID: 28114272, PMCID: PMC5308804, DOI: 10.1038/ncb3463.Peer-Reviewed Original Research
2016
Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells.
Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Nature Cell Biology 2016, 18: 1311-1323. PMID: 27842057, PMCID: PMC6159904, DOI: 10.1038/ncb3438.Peer-Reviewed Original ResearchFormin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse.
Murugesan S, Hong J, Yi J, Li D, Beach JR, Shao L, Meinhardt J, Madison G, Wu X, Betzig E, Hammer JA. Formin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse. The Journal Of Cell Biology 2016, 215: 383-399. PMID: 27799367, PMCID: PMC5100289, DOI: 10.1083/jcb.201603080.Peer-Reviewed Original ResearchHigh-density three-dimensional localization microscopy across large volumes.
Legant WR, Shao L, Grimm JB, Brown TA, Milkie DE, Avants BB, Lavis LD, Betzig E. High-density three-dimensional localization microscopy across large volumes. Nature Methods 2016, 13: 359-65. PMID: 26950745, PMCID: PMC4889433, DOI: 10.1038/nmeth.3797.Peer-Reviewed Original Research
2015
ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.
Li D, Shao L, Chen BC, Zhang X, Zhang M, Moses B, Milkie DE, Beach JR, Hammer JA, Pasham M, Kirchhausen T, Baird MA, Davidson MW, Xu P, Betzig E. ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science (New York, N.Y.) 2015, 349: aab3500. PMID: 26315442, PMCID: PMC4659358, DOI: 10.1126/science.aab3500.Peer-Reviewed Original ResearchMyosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.
Billington N, Beach JR, Heissler SM, Remmert K, Guzik-Lendrum S, Nagy A, Takagi Y, Shao L, Li D, Yang Y, Zhang Y, Barzik M, Betzig E, Hammer JA, Sellers JR. Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments. Current Biology : CB 2015, 25: 942-8. PMID: 25754640, DOI: 10.1016/j.cub.2015.02.012.Peer-Reviewed Original ResearchNonmuscle Myosin II Isoforms Coassemble in Living Cells.
Beach JR, Shao L, Remmert K, Li D, Betzig E, Hammer JA. Nonmuscle Myosin II Isoforms Coassemble in Living Cells. Current Biology : CB 2015, 25: 402. PMID: 29665398, DOI: 10.1016/j.cub.2015.01.028.Peer-Reviewed Original Research
2014
Practical Structured Illumination Microscopy
Rego EH, Shao L. Practical Structured Illumination Microscopy. Methods In Molecular Biology 2014, 1251: 175-192. PMID: 25391800, DOI: 10.1007/978-1-4939-2080-8_10.Peer-Reviewed Original ResearchLattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
Chen BC, Legant WR, Wang K, Shao L, Milkie DE, Davidson MW, Janetopoulos C, Wu XS, Hammer JA, Liu Z, English BP, Mimori-Kiyosue Y, Romero DP, Ritter AT, Lippincott-Schwartz J, Fritz-Laylin L, Mullins RD, Mitchell DM, Bembenek JN, Reymann AC, Böhme R, Grill SW, Wang JT, Seydoux G, Tulu US, Kiehart DP, Betzig E. Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution. Science (New York, N.Y.) 2014, 346: 1257998. PMID: 25342811, PMCID: PMC4336192, DOI: 10.1126/science.1257998.Peer-Reviewed Original Research