Featured Publications
Systems biochemistry to “deorphanize” human mitochondrial proteome
Miros F, Liu R, Shen H. Systems biochemistry to “deorphanize” human mitochondrial proteome. Molecular Cell 2022, 82: 2735-2737. PMID: 35931038, DOI: 10.1016/j.molcel.2022.07.005.Peer-Reviewed Original ResearchThe Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK. The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12. Cell 2017, 171: 771-782.e11. PMID: 29056341, PMCID: PMC5827971, DOI: 10.1016/j.cell.2017.09.051.Peer-Reviewed Original ResearchConceptsMethylmalonyl-CoA mutaseCitramalyl-CoA lyaseAnti-microbial metabolitesCell-autonomous defectSubstrate analogue inhibitorsMammalian cellsStructural biologyPathway assignmentItaconyl-CoAMitochondrial enzymesCellular activitiesMetabolic pathwaysDependent methylmalonyl-CoA mutaseHomozygous lossConsistent phenotypeConsequence of exposureVertebratesCellsEnzymologyBiologyMutaseLyasePhenotypeEnzymePathway
2013
Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors
Park RJ, Shen H, Liu L, Liu X, Ferguson SM, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. Journal Of Cell Science 2013, 126: 5305-5312. PMID: 24046449, PMCID: PMC3828596, DOI: 10.1242/jcs.138578.Peer-Reviewed Original ResearchConceptsDynamin 1Dynamin inhibitorTKO cellsPeripheral membrane rufflingDouble knockoutDynamin 3 geneClathrin-mediated endocytosisTriple-knockout cellsDynamin-dependent processFluid-phase endocytosisDyngo-4aMembrane fissionMembrane rufflingDKO cellsDynamin 2Knockout cellsLow-level expressionCell physiologyDynaminEndocytosisTarget effectsDKO fibroblastsGenesCellsKO fibroblasts
2009
Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits
Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Ferguson A, Destaing O, Ko G, Takasaki J, Cremona O, Toole E, De Camilli P. Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits. Developmental Cell 2009, 17: 811-822. PMID: 20059951, PMCID: PMC2861561, DOI: 10.1016/j.devcel.2009.11.005.Peer-Reviewed Original ResearchConceptsEndocytic roleEndocytic clathrin-coated pitsBAR domain proteinsSense membrane curvatureClathrin-coated pitsWild-type cellsGTPase dynaminEndocytic intermediatesHigher eukaryotesDomain proteinsMembrane fissionActin dynamicsNumerous proteinsDynaminMembrane curvatureProtein upstreamTubular neckConcerted actionProteinActinCoordinated actionKey playersFunctional relationshipCellsSequence of events