Featured Publications
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution
Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyUltralow light intensitiesSuperresolution imaging methodExcited statesFluorophore excited stateStructured illumination microscopyLight intensityRequired nonlinearityResolution extensionHigh light intensityActin cytoskeletonCellular structureReversible photoswitchingNuclear poresNonlinear responseIllumination intensitySuch nonlinear responsesPhotoswitchable proteinsSpatial resolutionFluorescent proteinBiological samplesSix-orderImaging methodMicroscopyPolystyrene beads
2011
PALM and STORM: Unlocking live‐cell super‐resolution
Henriques R, Griffiths C, Rego E, Mhlanga M. PALM and STORM: Unlocking live‐cell super‐resolution. Biopolymers 2011, 95: 322-331. PMID: 21254001, DOI: 10.1002/bip.21586.ChaptersConceptsLive-cell imaging approachesFluorescence light microscopySuper-resolution microscopySingle-molecule levelCellular biologyLocalization microscopy methodsLight microscopySuper-resolution imagingSingle-molecule localization microscopy methodsBiologyMolecule levelFluorescent markersRecent breakthroughsRecent advancesMicroscopy methodsLight microscopeImaging approachDynamic interactionMolecular visualizationPalmImportant toolCellsMicroscopyImportant goalMarkers