2022
Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism
Shamma F, Rego E, Boutte C. Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism. Molecular Microbiology 2022, 118: 47-60. PMID: 35670057, PMCID: PMC10070032, DOI: 10.1111/mmi.14951.Peer-Reviewed Original ResearchConceptsCell wall metabolismWall metabolismCell wall-related proteinsSerine/threonine proteinCell wall regulationPhosphomimetic mutationReversible phosphorylationThreonine phosphataseEnvironmental stressRegulatory proteinsCell wallCorresponding mutationMycobacterial cell wallAntibiotic toleranceNovel substrateFhaAProteinPstPMycobacterium smegmatisWag31Certain substratesPeptidoglycanPhosphorylationRegulationMajor mechanism
2020
The Conserved Translation Factor LepA Is Required for Optimal Synthesis of a Porin Family in Mycobacterium smegmatis
Fishbein S, Tomasi F, Wolf I, Dulberger C, Wang A, Keshishian H, Wallace L, Carr S, Ioerger T, Rego E, Rubin E. The Conserved Translation Factor LepA Is Required for Optimal Synthesis of a Porin Family in Mycobacterium smegmatis. Journal Of Bacteriology 2020, 203: 10.1128/jb.00604-20. PMID: 33361193, PMCID: PMC8095456, DOI: 10.1128/jb.00604-20.Peer-Reviewed Original ResearchMembrane-associated proteinsMembrane homeostasisCellular processesCell wallMembrane-associated proteomeWhole cell proteomeMajor membrane proteinOuter membrane porinsSteady-state abundanceDrug tolerance phenotypeMembrane proteomePorin familyTolerance phenotypeDeletion mutantsMembrane proteinsTranslation factorsMembrane porinsMajor porinsNutrient uptakeCell wall constituentsMycobacterial cell wallAntibiotic tolerancePorinProteomeDrug targets
2018
Rifampicin can induce antibiotic tolerance in mycobacteria via paradoxical changes in rpoB transcription
Zhu JH, Wang BW, Pan M, Zeng YN, Rego H, Javid B. Rifampicin can induce antibiotic tolerance in mycobacteria via paradoxical changes in rpoB transcription. Nature Communications 2018, 9: 4218. PMID: 30310059, PMCID: PMC6181997, DOI: 10.1038/s41467-018-06667-3.Peer-Reviewed Original Research