2004
Engineered riboregulators enable post-transcriptional control of gene expression
Isaacs FJ, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, Collins JJ. Engineered riboregulators enable post-transcriptional control of gene expression. Nature Biotechnology 2004, 22: 841-847. PMID: 15208640, DOI: 10.1038/nbt986.Peer-Reviewed Original ResearchConceptsStem-loop structureGene expressionPost-transcriptional controlPost-transcriptional regulation systemSmall noncoding RNAsTrans targetsCis-repressive sequenceRibosome bindingNoncoding RNAsEndogenous RNATarget genesCis sequencesRegulatory roleEscherichia coliRNABiological networksRiboregulatorsMechanistic actionGenesExpressionRecent studiesSequenceRegulation systemTranscriptionPromoter
2003
Prediction and measurement of an autoregulatory genetic module
Isaacs FJ, Hasty J, Cantor CR, Collins JJ. Prediction and measurement of an autoregulatory genetic module. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 7714-7719. PMID: 12808135, PMCID: PMC164653, DOI: 10.1073/pnas.1332628100.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationFlow CytometryGene Expression RegulationGenetic VariationGreen Fluorescent ProteinsLuminescent ProteinsModels, GeneticPhenotypePlasmidsSoftwareTemperatureConceptsGene regulatory networksGenetic modulesGene networksRegulatory networksComplex gene regulatory networksPhenotypic cellular responsesAutoregulatory gene networksStochastic versionCellular processesExpression statesProtein destabilizationCellular controlModel fluctuationsGene expressionSystems biologyCellular responsesProper quantitative descriptionQuantitative agreementSuch networksInternal noiseQuantitative descriptionQuantitative understandingKey propertiesPopulation distributionExperimental validation