2023
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factors
2018
Toward a unified picture of the exocytotic fusion pore
Karatekin E. Toward a unified picture of the exocytotic fusion pore. FEBS Letters 2018, 592: 3563-3585. PMID: 30317539, PMCID: PMC6353554, DOI: 10.1002/1873-3468.13270.Peer-Reviewed Original Research
2016
Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains
Wu Z, Auclair SM, Bello O, Vennekate W, Dudzinski NR, Krishnakumar SS, Karatekin E. Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Scientific Reports 2016, 6: 27287. PMID: 27264104, PMCID: PMC4893671, DOI: 10.1038/srep27287.Peer-Reviewed Original ResearchConceptsFusion poreTransmembrane domainPore dynamicsProtein transmembrane domainNeurotransmitter-filled vesiclesT-SNAREsPlasma membraneRecycling kineticsPore lifetimePore currentsFlickering poresPore stabilityMultiple timesZipperingNanodiscsDomainProteinVesiclesMembraneCellsAssaysCognatesPore propertiesPoresCholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores
Stratton BS, Warner JM, Wu Z, Nikolaus J, Wei G, Wagnon E, Baddeley D, Karatekin E, O’Shaughnessy B. Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. Biophysical Journal 2016, 110: 1538-1550. PMID: 27074679, PMCID: PMC4833774, DOI: 10.1016/j.bpj.2016.02.019.Peer-Reviewed Original ResearchConceptsT-SNAREsFusion poreSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsLipid bilayer propertiesFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsTotal internal reflection fluorescence microscopyCognate t-SNAREsFusion pore dynamicsPhysiological cholesterol levelsProtein receptor proteinsFlickering fusion poresReflection fluorescence microscopyIndividual fusion eventsV-SNAREBilayer propertiesFusion eventsPore flickeringReceptor proteinFluorescence microscopySmall unilamellar vesiclesLipid compositionFusion siteSnareExocytotic release
2010
A fast, single-vesicle fusion assay mimics physiological SNARE requirements
Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.Peer-Reviewed Original Research