2022
Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy
Khalilimeybodi A, Riaz M, Campbell S, Omens J, McCulloch A, Qyang Y, Saucerman J. Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy. Journal Of Molecular And Cellular Cardiology 2022, 174: 1-14. PMID: 36370475, PMCID: PMC10230857, DOI: 10.1016/j.yjmcc.2022.10.006.Peer-Reviewed Original Research
2020
Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffoldsInduced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing
Gorecka J, Gao X, Fereydooni A, Dash BC, Luo J, Lee SR, Taniguchi R, Hsia HC, Qyang Y, Dardik A. Induced pluripotent stem cell-derived smooth muscle cells increase angiogenesis and accelerate diabetic wound healing. Regenerative Medicine 2020, 15: 1277-1293. PMID: 32228292, PMCID: PMC7304438, DOI: 10.2217/rme-2019-0086.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsMuscle cellsDiabetic wound healingWound healingPro-angiogenic cytokinesMurine AdiposeStem cellsType macrophagesCollagen scaffoldsCultured mediumM2-type macrophagesCellsNumber of totalNew candidatesAngiogenesisNude miceDiabetic woundsPromising new candidateScaffoldsHealingCytokinesExpressionSecreteWoundsAdipose
2017
Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering
Luo J, Qin L, Kural MH, Schwan J, Li X, Bartulos O, Cong XQ, Ren Y, Gui L, Li G, Ellis MW, Li P, Kotton DN, Dardik A, Pober JS, Tellides G, Rolle M, Campbell S, Hawley RJ, Sachs DH, Niklason LE, Qyang Y. Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering. Biomaterials 2017, 147: 116-132. PMID: 28942128, PMCID: PMC5638652, DOI: 10.1016/j.biomaterials.2017.09.019.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsPluripotent stem cellsFunctional vascular smooth muscle cellsMassachusetts General Hospital miniature swineMuscle cellsSelf-assembly approachBiodegradable polyglycolic acid (PGA) scaffoldsPrimary vascular smooth muscle cellsSmooth muscle myosin heavy chainMuscle myosin heavy chainVascular tissue engineeringStem cellsTissue engineeringPolyglycolic acid scaffoldsReprogramming factorsVascular diseaseContractile functionVascular constructsImmunodeficient miceOrgan transplantsMiniature swinePreclinical investigationsGreat potentialMyosin heavy chain
2014
Functional Cardiomyocytes Derived from Isl1 Cardiac Progenitors via Bmp4 Stimulation
Cagavi E, Bartulos O, Suh CY, Sun B, Yue Z, Jiang Z, Yue L, Qyang Y. Functional Cardiomyocytes Derived from Isl1 Cardiac Progenitors via Bmp4 Stimulation. PLOS ONE 2014, 9: e110752. PMID: 25522363, PMCID: PMC4270687, DOI: 10.1371/journal.pone.0110752.Peer-Reviewed Original ResearchConceptsCardiac progenitor cellsCardiac repairProliferative abilityProgenitor cellsCell-based cardiac regenerative therapySignificant therapeutic valueCardiac regenerative therapyAdult cardiac progenitor cellsStem cellsCPC culturesHigh proliferative abilityHeart failureCardiac originCardiac differentiationMyocardial infarctionAnterior heart fieldInduction of Bmp4Leading causePotential treatmentRole of BMP4Therapeutic valueCardiac progenitorsUntreated cardiomyocytesProtein expressionRegenerative therapy
2012
Modeling Supravalvular Aortic Stenosis Syndrome With Human Induced Pluripotent Stem Cells
Ge X, Ren Y, Bartulos O, Lee MY, Yue Z, Kim KY, Li W, Amos PJ, Bozkulak EC, Iyer A, Zheng W, Zhao H, Martin KA, Kotton DN, Tellides G, Park IH, Yue L, Qyang Y. Modeling Supravalvular Aortic Stenosis Syndrome With Human Induced Pluripotent Stem Cells. Circulation 2012, 126: 1695-1704. PMID: 22914687, PMCID: PMC3586776, DOI: 10.1161/circulationaha.112.116996.Peer-Reviewed Original ResearchConceptsActin filament bundlesSmooth muscle αSmooth muscle cellsExtracellular signal-regulated kinase 1/2Muscle αFilament bundlesSignal-regulated kinase 1/2Four-nucleotide insertionDisease mechanismsContractile smooth muscle cellsStem cell linesPluripotent stem cellsPluripotent stem cell linePlatelet-derived growth factorRhoA signalingVascular smooth muscle cellsRecombinant proteinsKinase 1/2Elastin geneELN geneWilliams-Beuren syndromeBrdU analysisSupravalvular aortic stenosisStem cellsHigh proliferation rate
2011
High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells
Lee MY, Bozkulak E, Schliffke S, Amos PJ, Ren Y, Ge X, Ehrlich BE, Qyang Y. High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells. Biochemical And Biophysical Research Communications 2011, 416: 51-57. PMID: 22079290, PMCID: PMC3237870, DOI: 10.1016/j.bbrc.2011.10.140.Peer-Reviewed Original ResearchConceptsMurine embryonic stem cellsEmbryonic stem cellsBone morphogenetic proteinEB culturesCardiac differentiationCardiomyocyte productionEmbryoid body culturesStem cellsCardiac-specific genesEarly cardiac developmentCardiogenic growth factorsNK2 transcription factorCardiac regenerative medicineMESC linesTranscription factorsCardiac developmentMorphogenetic proteinsEmbryoid bodiesDifferentiation systemDifferentiation periodFunctional cardiomyocytesHigh-density culturesMyosin light chain 2vRegenerative medicineCell-based therapiesDerivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells
Lee MY, Sun B, Schliffke S, Yue Z, Ye M, Paavola J, Bozkulak EC, Amos PJ, Ren Y, Ju R, Jung YW, Ge X, Yue L, Ehrlich BE, Qyang Y. Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells. Stem Cell Research 2011, 8: 49-57. PMID: 22099020, PMCID: PMC3222859, DOI: 10.1016/j.scr.2011.08.004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdherens JunctionsAnimalsBacterial ProteinsBase SequenceCadherinsCalciumCell Culture TechniquesCell LineCell SeparationConnexin 43Embryonic Stem CellsFlow CytometryGap JunctionsHeart VentriclesImaging, Three-DimensionalIntegrasesLuminescent ProteinsMiceMyocytes, CardiacMyosin Light ChainsPromoter Regions, GeneticProteinsRNA, UntranslatedConceptsVentricular cardiomyocytesCalcium transientsNeonatal mouse ventricular cardiomyocytesFunctional excitation-contraction couplingCardiac contractile performanceDouble transgenic miceCurrent-clamp recordingsIntracellular calcium transientsExcitation-contraction couplingAction potential characteristicsMouse ventricular cardiomyocytesMyosin light chain 2vFluorescence-activated cell sortingAdrenergic signalingIntracellular calciumContractile performanceClamp recordingsTransgenic miceElectrical stimulationCardiac repairInduction of differentiationIsoproterenol stimulationExpression of YFPMouse linesN-cadherinSmall molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells
Ren Y, Lee MY, Schliffke S, Paavola J, Amos PJ, Ge X, Ye M, Zhu S, Senyei G, Lum L, Ehrlich BE, Qyang Y. Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells. Journal Of Molecular And Cellular Cardiology 2011, 51: 280-287. PMID: 21569778, PMCID: PMC3334336, DOI: 10.1016/j.yjmcc.2011.04.012.Peer-Reviewed Original ResearchConceptsHuman iPS cellsAction potential durationHuman iPS cell-derived cardiomyocytesSmall-molecule Wnt inhibitorsIPS cellsPluripotent stem cellsIPS cell-derived cardiomyocytesBMP-4Cardiac disease mechanismsCardiac differentiationCell-derived cardiomyocytesPotential durationBone morphogenetic protein 4Stem cellsWnt inhibitorsCardiomyocyte action potential durationHuman pluripotent stem cellsInduced pluripotent stem cellsHuman induced pluripotent stem cellsDisease mechanismsTranslational cardiac researchIntracellular calcium imagingEfficient cardiac differentiationWnt/β-cateninProduction of cardiomyocytes
2007
The Renewal and Differentiation of Isl1 + Cardiovascular Progenitors Are Controlled by a Wnt/β-Catenin Pathway
Qyang Y, Martin-Puig S, Chiravuri M, Chen S, Xu H, Bu L, Jiang X, Lin L, Granger A, Moretti A, Caron L, Wu X, Clarke J, Taketo MM, Laugwitz KL, Moon RT, Gruber P, Evans SM, Ding S, Chien KR. The Renewal and Differentiation of Isl1 + Cardiovascular Progenitors Are Controlled by a Wnt/β-Catenin Pathway. Cell Stem Cell 2007, 1: 165-179. PMID: 18371348, DOI: 10.1016/j.stem.2007.05.018.Peer-Reviewed Original Research
2004
Myeloproliferative Disease in Mice with Reduced Presenilin Gene Dosage: Effect of γ-Secretase Blockage †
Qyang Y, Chambers SM, Wang P, Xia X, Chen X, Goodell MA, Zheng H. Myeloproliferative Disease in Mice with Reduced Presenilin Gene Dosage: Effect of γ-Secretase Blockage †. Biochemistry 2004, 43: 5352-5359. PMID: 15122901, DOI: 10.1021/bi049826u.Peer-Reviewed Original ResearchMeSH KeywordsAmyloid Precursor Protein SecretasesAnimalsAspartic Acid EndopeptidasesCell LineageColony-Forming Units AssayEndopeptidasesFemaleGene DosageGranulocytesHematopoiesisLeukocyte CountMacrophagesMaleMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMyeloproliferative DisordersPresenilin-1Presenilin-2Protease InhibitorsConceptsGamma-secretase activityDisease interventionWild-type splenocytesAlzheimer's disease interventionsAmyloid precursor proteinGranulocyte-macrophage colony-forming unitsGamma-secretase inhibitorsGamma-secretase cleavageGranulocyte infiltrationPotential therapyMyeloproliferative diseaseB lymphocytesBone marrowPS inactivationHematopoietic stem cellsColony-forming unitsGranulocytic cells
2001
Genetic and Molecular Characterization of Skb15, a Highly Conserved Inhibitor of the Fission Yeast PAK, Shk1
Kim H, Yang P, Qyang Y, Lai H, Du H, Henkel J, Kumar K, Bao S, Liu M, Marcus S. Genetic and Molecular Characterization of Skb15, a Highly Conserved Inhibitor of the Fission Yeast PAK, Shk1. Molecular Cell 2001, 7: 1095-1101. PMID: 11389855, DOI: 10.1016/s1097-2765(01)00248-9.Peer-Reviewed Original ResearchConceptsFission yeastP21-activated kinaseCytokinetic functionCytokinetic machineryCell polarityProtein functionSkb15Cytoskeletal remodelingMouse homologMicrotubule biogenesisShk1Mating responseNegative regulationActin polymerizationNull mutationCell cycleMolecular characterizationYeastSubstantial uncouplingProper executionPartial lossSchizosaccharomycesBiogenesisHomologKinase