2021
Loss of crossbridge inhibition drives pathological cardiac hypertrophy in patients harboring the TPM1 E192K mutation
Sewanan LR, Park J, Rynkiewicz MJ, Racca AW, Papoutsidakis N, Schwan J, Jacoby DL, Moore JR, Lehman W, Qyang Y, Campbell SG. Loss of crossbridge inhibition drives pathological cardiac hypertrophy in patients harboring the TPM1 E192K mutation. The Journal Of General Physiology 2021, 153: e202012640. PMID: 34319370, PMCID: PMC8321830, DOI: 10.1085/jgp.202012640.Peer-Reviewed Original ResearchConceptsHypertrophic cardiomyopathyHeart tissueCellular hypertrophyEngineered Heart TissuePathological cardiac hypertrophyThin filament mutationsMavacamten treatmentDiastolic dysfunctionDisease featuresHypertrophic effectCardiac hypertrophyContractile differencesHypertrophyFundamental disease mechanismsCrossbridge activityInherited disorderOverall Ca2Uncertain significancePatient phenotypesDisease mechanismsLow Ca2PatientsK mutationMavacamtenTissue
2020
Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues
Ng R, Sewanan LR, Brill AL, Stankey P, Li X, Qyang Y, Ehrlich BE, Campbell SG. Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues. AJP Heart And Circulatory Physiology 2020, 318: h1516-h1524. PMID: 32383992, PMCID: PMC7311697, DOI: 10.1152/ajpheart.00055.2020.Peer-Reviewed Original ResearchConceptsMitochondrial proteinsMitochondrial massMitochondrial protein levelsMitochondrial protein expressionMyosin ATPase inhibitorMitochondrial biogenesisExtended tissue culturePathological cardiac adaptationATP demandActive length controlFull recapitulationBiogenesisLength controlHeart tissueProtein levelsATPase inhibitorProtein expressionCulture environmentProteinTissue cultureCardiac physiologyNovel bioreactorVivo conditionsContractile workVitro method
2016
ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction
Bartulos O, Zhuang ZW, Huang Y, Mikush N, Suh C, Bregasi A, Wang L, Chang W, Krause DS, Young LH, Pober JS, Qyang Y. ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction. JCI Insight 2016, 1: e80920. PMID: 27525311, PMCID: PMC4982472, DOI: 10.1172/jci.insight.80920.Peer-Reviewed Original ResearchMyocardial infarctionControl animalsCardiovascular progenitor cellsProgenitor cellsVentricular contractile functionCardiac repair strategiesNew blood vesselsInfarct areaLineage-tracing studiesContractile functionCardiac repairBlood vessel formationMyocardial regenerationEndothelial cellsHeart tissueBlood vesselsMurine heartInfarctionVessel formationInjuryMiceDelivery approachCardiomyocytesHeartCells