2019
Dynamic instability of clathrin assembly provides proofreading control for endocytosis
Chen Y, Yong J, Martínez-Sánchez A, Yang Y, Wu Y, De Camilli P, Fernández-Busnadiego R, Wu M. Dynamic instability of clathrin assembly provides proofreading control for endocytosis. Journal Of Cell Biology 2019, 218: 3200-3211. PMID: 31451612, PMCID: PMC6781453, DOI: 10.1083/jcb.201804136.Peer-Reviewed Original ResearchConceptsClathrin-coated pitsCell-free reconstitution systemClathrin coat formationSingle-molecule imagingAssembly machineryCargo sortingATPase Hsc70Hsc70 functionClathrin assemblyCoat formationCargo incorporationReconstitution systemDynamic exchangeProofreading mechanismClathrinEndocytosisAssembly rateCargoCheckpoint
2017
Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning
Yang Y, Xiong D, Pipathsouk A, Weiner OD, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Developmental Cell 2017, 43: 507-521.e4. PMID: 29161594, PMCID: PMC5826602, DOI: 10.1016/j.devcel.2017.10.028.Peer-Reviewed Original ResearchConceptsPlasma membraneClathrin assemblyClathrin functionEndocytic proteinsMembrane traffickingEndocytic machineryN-WASPEndocytic eventsSignal transductionDownstream factorsCell surfaceCortical patterningPattern formationAssemblyMembraneImportant insightsActive processSingle-cell patternFBP17Cdc42ClathrinEndocytosisTransductionCortical pattern formationPhosphatidylinositol
2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds