2020
Noggin regulates foregut progenitor cell programming and mis-expression leads to esophageal atresia
Pinzon-Guzman C, Sangadala S, Riera KM, Popova EY, Manning E, Huh WJ, Alexander MS, Shelton JS, Boden SD, Goldenring JR. Noggin regulates foregut progenitor cell programming and mis-expression leads to esophageal atresia. Journal Of Clinical Investigation 2020, 130: 4396-4410. PMID: 32427591, PMCID: PMC7410075, DOI: 10.1172/jci123597.Peer-Reviewed Original Research
2019
Heterogeneity within Stratified Epithelial Stem Cell Populations Maintains the Oral Mucosa in Response to Physiological Stress
Byrd KM, Piehl NC, Patel JH, Huh WJ, Sequeira I, Lough KJ, Wagner BL, Marangoni P, Watt FM, Klein OD, Coffey RJ, Williams SE. Heterogeneity within Stratified Epithelial Stem Cell Populations Maintains the Oral Mucosa in Response to Physiological Stress. Cell Stem Cell 2019, 25: 814-829.e6. PMID: 31809739, PMCID: PMC6925542, DOI: 10.1016/j.stem.2019.11.005.Peer-Reviewed Original ResearchConceptsStem cell populationJunctional zoneOral mucosaStem cellsImportant model systemCell populationsEpithelial stem cell populationSingle-progenitor modelAsymmetric divisionLabel-retention assaysCandidate nichesSymmetric divisionTissue stressOral cavityPalatal epitheliumPhysiological stressModel systemStratified epitheliumMucosaProliferative heterogeneityEpithelium
2010
XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum
Huh WJ, Esen E, Geahlen JH, Bredemeyer AJ, Lee A, Shi G, Konieczny SF, Glimcher LH, Mills JC. XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum. Gastroenterology 2010, 139: 2038-2049. PMID: 20816838, PMCID: PMC2997137, DOI: 10.1053/j.gastro.2010.08.050.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineChief Cells, GastricDNA-Binding ProteinsEndoplasmic Reticulum, RoughIntegrasesMiceMice, KnockoutMicroscopy, Electron, TransmissionPromoter Regions, GeneticRegulatory Factor X Transcription FactorsSecretory VesiclesStem CellsTranscription FactorsX-Box Binding Protein 1ConceptsEndoplasmic reticulumZymogenic cellsRough endoplasmic reticulumNC markersGastric zymogenic cellsTranscription factor XBP1Large secretory vesiclesLamellar rough endoplasmic reticulumAbsence of XBP1Transcription factor MIST1Cell shape abnormalitiesCre-loxP systemTranscriptional regulationChromatin immunoprecipitationTranscriptional activationSecretory vesiclesGastric cell linesMist1Neck cellsXBP1Cell typesImmunoblot analysisCell linesQuantitative reverse transcriptase-polymerase chain reactionMIST1 expression
2009
How form follows functional genomics: gene expression profiling gastric epithelial cells with a particular discourse on the parietal cell
Capoccia BJ, Huh WJ, Mills JC. How form follows functional genomics: gene expression profiling gastric epithelial cells with a particular discourse on the parietal cell. Physiological Genomics 2009, 37: 67-78. PMID: 19208773, PMCID: PMC2685495, DOI: 10.1152/physiolgenomics.90408.2008.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsFunctional genomic studiesFunctional genomicsGene expressionGenomic studiesCell lineagesEntire cellular transcriptomeGene ontology analysisDifferentiated cell lineagesGene expression patternsCellular transcriptomeParietal cellsVascular endothelial growth factor BIndividual genesLineage-specific markersOntology analysisCell physiologyGene productsMolecular anatomyGastric parietal cellsExpression patternsMicroarray analysisMolecular mechanismsCell-specific markersGastric epithelial cellsGrowth factor B
2008
The gastric epithelial progenitor cell niche and differentiation of the zymogenic (chief) cell lineage
Bredemeyer AJ, Geahlen JH, Weis VG, Huh WJ, Zinselmeyer BH, Srivatsan S, Miller MJ, Shaw AS, Mills JC. The gastric epithelial progenitor cell niche and differentiation of the zymogenic (chief) cell lineage. Developmental Biology 2008, 325: 211-224. PMID: 19013146, PMCID: PMC2634829, DOI: 10.1016/j.ydbio.2008.10.025.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBasic Helix-Loop-Helix Transcription FactorsCadherinsCell DifferentiationCell LineageCell PolarityCell ProliferationChief Cells, GastricCytoskeletal ProteinsEpithelial CellsIntercellular JunctionsMiceModels, BiologicalParietal Cells, GastricProtein BindingProtein TransportStem Cell NicheStem CellsConceptsZymogenic cellsProgenitor nicheCell fate decisionsE-cadherin localizationZymogenic cell lineagesMammalian gastrointestinal tractProgenitor cell nicheParietal cellsLineage progenitor cellsKey cellular componentsFate decisionsDiverse lineagesProgenitor stateDifferentiated cell markersCell nicheGastric parietal cellsCell lineagesPremature expressionCellular componentsGastric unitsLineagesNicheGenetic ablationLineage cellsProgenitor cells
2006
Location, allocation, relocation: isolating adult tissue stem cells in three dimensions
Huh WJ, Pan XO, Mysorekar IU, Mills JC. Location, allocation, relocation: isolating adult tissue stem cells in three dimensions. Current Opinion In Biotechnology 2006, 17: 511-517. PMID: 16889955, DOI: 10.1016/j.copbio.2006.07.002.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements