2024
An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus
Sagawa C, Thomson G, Mermaz B, Vernon C, Liu S, Jacob Y, Irish V. An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus. Plant Methods 2024, 20: 148. PMID: 39342225, PMCID: PMC11438372, DOI: 10.1186/s13007-024-01274-4.Peer-Reviewed Original ResearchSimultaneous editing of multiple genesMultiple genesGene editingEudicot plant speciesPol III promotersTarget multiple genesGenus fallRPS5A promoterCRISPR/Cas9 gene editingCRISPR/Cas9-mediated gene editingMultiplex gene editingGenome engineeringIII promotersGenetic screeningPlant speciesCas9 endonucleaseEditing efficiencyGene editing efficiencySgRNAGenesArabidopsisUBQ10SpeciesSimultaneous editingPromoterOptimization of in planta methodology for genome editing and transformation in Citrus
Khadgi A, Sagawa C, Vernon C, Mermaz B, Irish V. Optimization of in planta methodology for genome editing and transformation in Citrus. Frontiers In Plant Science 2024, 15: 1438031. PMID: 39070914, PMCID: PMC11272483, DOI: 10.3389/fpls.2024.1438031.Peer-Reviewed Original ResearchCitrus cultivarsCommercial citrus cultivarsPlanta transformation protocolPineapple sweet orangeTissue cultureGene-edited linesLisbon lemonSweet orangeCulture-based approachesPlanta transformationRecalcitrant speciesCitrus plantsCultivarsGenetic transformationTransformation protocolPlant speciesCRISPR/Cas9 constructsTransformation efficiencyCitrusGenetic changesGenome editingAseptic conditionsRegeneration rateLemonPlants
2017
The ABC model of floral development
Irish V. The ABC model of floral development. Current Biology 2017, 27: r887-r890. PMID: 28898659, DOI: 10.1016/j.cub.2017.03.045.Peer-Reviewed Original ResearchRhamnose-Containing Cell Wall Polymers Suppress Helical Plant Growth Independently of Microtubule Orientation
Saffer AM, Carpita NC, Irish VF. Rhamnose-Containing Cell Wall Polymers Suppress Helical Plant Growth Independently of Microtubule Orientation. Current Biology 2017, 27: 2248-2259.e4. PMID: 28736166, DOI: 10.1016/j.cub.2017.06.032.Peer-Reviewed Original ResearchConceptsCell wall polymersPlant growthWall polymersMicrotubule orientationPectic polysaccharide rhamnogalacturonanHelical growthMost plant organsEpidermal cell expansionCell wall compositionCortical microtubule arraysPetal epidermal cellsMutant rootsPlant cellsPlant speciesRhamnose synthasePlant organsWall compositionMicrotubule arraysEpidermal cellsCell expansionImportant functionsMutantsNovel sourceMutationsSpecific organs
2008
An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development
Chae E, Tan Q, Hill TA, Irish VF. An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development. Development 2008, 135: 1235-1245. PMID: 18287201, DOI: 10.1242/dev.015842.Peer-Reviewed Original ResearchConceptsUNUSUAL FLORAL ORGANSAP3 promoterLFY activityTranscription factorsProtein degradationFloral homeotic gene expressionLEAFY transcription factorFloral homeotic genesHomeotic gene expressionTranscriptional repressor domainF-box proteinsSCF ubiquitin ligaseF-box componentAPETALA3 (AP3) geneHomeotic genesRepressor domainFloral organsFlower developmentPlant speciesTranscriptional complexPlants flowerProtein actsFloral developmentUbiquitin ligaseEndogenous signals
1991
Cell lineage in plant development
Irish V. Cell lineage in plant development. Current Opinion In Cell Biology 1991, 3: 983-987. PMID: 1814369, DOI: 10.1016/0955-0674(91)90117-h.Peer-Reviewed Original ResearchCell lineage in plant development
Irish V. Cell lineage in plant development. Current Opinion In Genetics & Development 1991, 1: 169-173. PMID: 1822266, DOI: 10.1016/s0959-437x(05)80065-6.Peer-Reviewed Original Research