2024
Optimization of in planta methodology for genome editing and transformation in Citrus
Khadgi A, Sagawa C, Vernon C, Mermaz B, Irish V. Optimization of in planta methodology for genome editing and transformation in Citrus. Frontiers In Plant Science 2024, 15: 1438031. PMID: 39070914, PMCID: PMC11272483, DOI: 10.3389/fpls.2024.1438031.Peer-Reviewed Original ResearchCitrus cultivarsCommercial citrus cultivarsPlanta transformation protocolPineapple sweet orangeTissue cultureGene-edited linesLisbon lemonSweet orangeCulture-based approachesPlanta transformationRecalcitrant speciesCitrus plantsCultivarsGenetic transformationTransformation protocolPlant speciesCRISPR/Cas9 constructsTransformation efficiencyCitrusGenetic changesGenome editingAseptic conditionsRegeneration rateLemonPlants
2017
Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress
LeBlanc C, Zhang F, Mendez J, Lozano Y, Chatpar K, Irish V, Jacob Y. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress. The Plant Journal 2017, 93: 377-386. PMID: 29161464, DOI: 10.1111/tpj.13782.Peer-Reviewed Original ResearchConceptsCRISPR/Green fluorescent protein (GFP) reporter geneCRISPR/Cas9 systemFluorescent protein reporter geneCRISPR/Cas9Off-target mutationsArabidopsis plantsEukaryotic genomesDifferent organismsSomatic tissuesCitrus plantsCas9 systemDNA breaksReporter geneTarget mutagenesisTargeted mutationsMutation rateMutagenesisImportance of temperatureArabidopsisHeat stressPlantsMutationsQuantitative assayEukaryotes
2014
A dexamethasone-inducible gene expression system is active in Citrus plants
Rossignol P, Orbović V, Irish V. A dexamethasone-inducible gene expression system is active in Citrus plants. Scientia Horticulturae 2014, 172: 47-53. DOI: 10.1016/j.scienta.2014.02.041.Peer-Reviewed Original ResearchGene expression systemExpression systemΒ-glucuronidase (GUS) reporter geneInducible gene expression systemSynthetic transcription factorsGene of interestPromoter driving expressionCrop plantsGene functionTransgenic approachesTranscription factorsCitrus plantsNew traitsReporter geneCitrus cultivarsPlantsTransgene activityGenesSynthetic glucocorticoid dexamethasoneTiming of activityGlucocorticoid receptorSuch manipulationsGlucocorticoid dexamethasoneLhGRTraits