2016
Loss of Cbl-PI3K interaction modulates the periosteal response to fracture by enhancing osteogenic commitment and differentiation
Scanlon V, Walia B, Yu J, Hansen M, Drissi H, Maye P, Sanjay A. Loss of Cbl-PI3K interaction modulates the periosteal response to fracture by enhancing osteogenic commitment and differentiation. Bone 2016, 95: 124-135. PMID: 27884787, PMCID: PMC5819877, DOI: 10.1016/j.bone.2016.11.020.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsBiomarkersCell CountCell DifferentiationCell LineageCell NucleusCell ProliferationFracture HealingFractures, BoneMesodermMice, Inbred C57BLMutationOsteogenesisPeriosteumPhosphatidylinositol 3-KinasePhosphorylationProtein BindingProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-cblSp7 Transcription FactorUp-RegulationConceptsCbl-PI3K interactionUbiquitin ligase functionMultipotent skeletal progenitorsPeriosteal cellsPI3KMajor adaptor proteinP85 regulatory subunitTranscriptional target genesE3 ubiquitin ligaseOsteogenic differentiationPhosphatidylinositol-3 kinasePI3K regulationMajor signaling proteinsK interactionPI3K activityPeriosteal thickeningLipid kinasesP85 subunitSkeletal progenitorsAdaptor proteinRegulatory subunitLigase functionSignaling proteinsUbiquitin ligaseNuclear localizationIncreased periosteal expansion, Osterix expression and osteogenic potential upon bone injury during perturbed PI3K signaling
Walia B, Scanlon V, Yu J, Maye P, Drissi H, Sanjay A. Increased periosteal expansion, Osterix expression and osteogenic potential upon bone injury during perturbed PI3K signaling. Bone Abstracts 2016 DOI: 10.1530/boneabs.5.p75.Peer-Reviewed Original Research
2015
Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair
Scanlon V, Soung do Y, Adapala NS, Morgan E, Hansen MF, Drissi H, Sanjay A. Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair. PLOS ONE 2015, 10: e0138194. PMID: 26393915, PMCID: PMC4578922, DOI: 10.1371/journal.pone.0138194.Peer-Reviewed Original ResearchConceptsCbl-PI3K interactionHistomorphometric analysisFracture callusBone formationMid-diaphyseal femoral fracturePI3KNormal bone homeostasisBony callus formationSoft callus formationHealing femursFemoral fracturesWT miceOsteoclast numberBone resorptionTomography scanMurine modelOsteoblast surfaceFracture healingBone homeostasisBone volumeBone repairSkeletal remodelingMiceBiomechanical testingHigh expression