2023
Characterization of the distribution and dynamics of chromatin states in the C. elegans germ line reveals substantial H3K4me3 remodeling during oogenesis
Mazzetto M, Gonzalez L, Sanchez N, Reinke V. Characterization of the distribution and dynamics of chromatin states in the C. elegans germ line reveals substantial H3K4me3 remodeling during oogenesis. Genome Research 2023, 34: gr.278247.123. PMID: 38164610, PMCID: PMC10903938, DOI: 10.1101/gr.278247.123.Peer-Reviewed Original ResearchEpigenetic regulatory mechanismsGerm lineChromatin stateGerm cellsC. elegans germ lineGene expressionRegulatory mechanismsDevelopmental transitionsActive histone modification H3K4me3Most genomic studiesZygotic genome activationSmall RNA pathwaysSpatio-temporal expression patternsHistone modifications H3K4me3Chromatin accessibility dataGerm cell differentiationOverall transcript abundanceUndifferentiated germ cellsGenome activationRNA pathwaysChromatin landscapeChromatin organizationChromatin structureEssential genesHistone modifications
2021
The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline
McManus CE, Mazzetto M, Wei G, Han M, Reinke V. The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline. G3: Genes, Genomes, Genetics 2021, 11: jkab329. PMID: 34519784, PMCID: PMC8664474, DOI: 10.1093/g3journal/jkab329.Peer-Reviewed Original ResearchConceptsH3K36me3 levelsAutosomal lociC. elegans germ lineGerm cellsC. elegans germlineActive chromatin stateHistone modification patternsMES-4Chromatin stateEpigenetic balanceMutant phenotypeCaenorhabditis elegansSex chromosomesGenomic domainsGerm lineEfficient splicingDifferentiation programX chromosomeGenetic informationModification patternsTranscript integrityEfficient mRNANovel modifierH3K36me3Downstream effects
2019
Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression
Han M, Wei G, McManus CE, Hillier LW, Reinke V. Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression. BMC Genomics 2019, 20: 500. PMID: 31208332, PMCID: PMC6580472, DOI: 10.1186/s12864-019-5893-9.Peer-Reviewed Original ResearchConceptsHistone modificationsGerm cellsC. elegansC. elegans germ cellsGene expressionDevelopmental gene expressionGene expression mechanismsIsolation of nucleiGene regulationTranscriptome analysisChromatin immunoprecipitationGerm lineGenomic analysisDistinct genomic profilesRegulatory mechanismsRepressive mechanismsExpression mechanismElegansPure populationsGenomic profilesConsequent discoveriesCellsExpressionGenomeNucleus
2018
The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans
McManus CE, Reinke V. The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans. Genetics 2018, 208: 549-563. PMID: 29167199, PMCID: PMC5788521, DOI: 10.1534/genetics.117.1123.Peer-Reviewed Original ResearchConceptsGerm cell developmentGerm cellsCell developmentGermline-expressed genesNovel nuclear proteinCell cycle checkpointsGermline expressionGenomic integrityUnique fateOocyte differentiationFaithful transmissionCycle checkpointsNuclear proteinsMature gametesX chromosomeDevelopmental eventsLarval stagesMaturation eventsGenetic materialCaenorhabditisFactor 1Developmental progressionSubtle defectsCellsExpression
2011
C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival
Kapelle WS, Reinke V. C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival. Genesis 2011, 49: 380-391. PMID: 21305687, DOI: 10.1002/dvg.20726.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCell ProliferationCell SurvivalDisorders of Sex DevelopmentEmbryo, NonmammalianEpistasis, GeneticFemaleGene Expression Regulation, DevelopmentalGerm CellsLuminescent ProteinsMaleRNA InterferenceRNA-Binding ProteinsTime FactorsConceptsGerm cell proliferationMeg 1Germ cellsEmbryonic primordial germ cellsTargeted RNAi screenPrimordial germ cellsCell proliferationGerm cell survivalGerm lineageP granulesRNAi screenLarval stagesMEG 2Cell survivalFamily membersMultiple pathwaysGerm cell degenerationPhenotypeSterilityProliferationOptimal proliferationCellsCell degenerationEmbryogenesisLineages
2010
Genome-wide analysis of germ cell proliferation in C . elegans identifies VRK-1 as a key regulator of CEP-1/p53
Waters K, Yang AZ, Reinke V. Genome-wide analysis of germ cell proliferation in C . elegans identifies VRK-1 as a key regulator of CEP-1/p53. Developmental Biology 2010, 344: 1011-1025. PMID: 20599896, PMCID: PMC3375680, DOI: 10.1016/j.ydbio.2010.06.022.Peer-Reviewed Original ResearchConceptsGerm cell proliferationVRK-1Germ cellsCEP-1CEP-1/p53Cell proliferationImportant regulatory relationshipsGenome-wide analysisGene expression profilingGermline proliferationCell cycle arrestUseful model systemC. elegansProliferation defectFunctional characterizationNegative regulationExpression profilingRegulatory relationshipsKey regulatorP53 activityCycle arrestUnsuspected mechanismElegansModel systemProliferation
2009
DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans
Chi W, Reinke V. DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans. Cells And Development 2009, 126: 406-416. PMID: 19368797, PMCID: PMC2680456, DOI: 10.1016/j.mod.2009.01.008.Peer-Reviewed Original ResearchConceptsEFL-1/DPLDpl-1Germ lineTissue-specific rescue experimentsGonad armsItr-1RME-2Caenorhabditis elegansEfl-1C. elegansGene functionTranscriptional programsInositol triphosphate receptorGonad structureSuccessful reproductionSevere defectsRescue experimentsGerm cellsFunction mutationsSuccessful fertilizationLikely disruptsMutantsSpermathecaTriphosphate receptorElegans
2008
A C. elegans Piwi, PRG-1, Regulates 21U-RNAs during Spermatogenesis
Wang G, Reinke V. A C. elegans Piwi, PRG-1, Regulates 21U-RNAs during Spermatogenesis. Current Biology 2008, 18: 861-867. PMID: 18501605, PMCID: PMC2494713, DOI: 10.1016/j.cub.2008.05.009.Peer-Reviewed Original ResearchConceptsPRG-1Small RNAsC. elegansGerm cellsSubset of mRNAsPRG 2Cell totipotencyPIWI proteinsP granulesRibonucleoprotein granulesEpigenetic regulationSpecific family membersGerm linePiwi familyProper expressionPiwiSuccessful spermatogenesisDiverse classPiRNAsRNAElegansProteinSpermatogenesisExpressionFamily membersMEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans
Leacock SW, Reinke V. MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans. Genetics 2008, 178: 295-306. PMID: 18202375, PMCID: PMC2206079, DOI: 10.1534/genetics.107.080218.Peer-Reviewed Original ResearchConceptsP granulesMeg 1Germline developmentGerm lineageMEG 2Embryonic germ lineagesP-granule componentEmbryonic cell divisionGerm cell proliferationGLH-1Germ granulesSomatic lineagesCaenorhabditis elegansGermline segregationMis-segregationMaternal germlineCell divisionGerm cellsPhenotype increasesLineagesGermline defectsPGL-1MutantsProliferationGranules