2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationEpitheliumHairHair FollicleMiceTransforming Growth Factor betaConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCellsLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineageHair FollicleHomeostasisMice, KnockoutMice, TransgenicSingle-Cell AnalysisStem CellsConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured conditionHomeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation
Mesa KR, Kawaguchi K, Cockburn K, Gonzalez D, Boucher J, Xin T, Klein AM, Greco V. Homeostatic Epidermal Stem Cell Self-Renewal Is Driven by Local Differentiation. Cell Stem Cell 2018, 23: 677-686.e4. PMID: 30269903, PMCID: PMC6214709, DOI: 10.1016/j.stem.2018.09.005.Peer-Reviewed Original Research
2016
MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis
Yang H, Li M, Hu X, Xin T, Zhang S, Zhao G, Xuan T, Li M. MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis. Developmental Biology 2016, 416: 312-323. PMID: 27339292, DOI: 10.1016/j.ydbio.2016.06.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryonic Germ CellsFemaleGene Expression Regulation, DevelopmentalGene Knockdown TechniquesLarvaLuminescent ProteinsMicroRNAsMicroscopy, FluorescenceOrganogenesisOvaryRibonuclease IIIRNA InterferenceRNA-Binding ProteinsStem Cell NicheConceptsOvary morphogenesisPrimordial germ cellsLate third larval instarLoss of DroshaMiRNA pathway componentsCanonical miRNA pathwayGerm cell lineageMiRNA-mediated regulationGerm cell precursorsGenome-wide screeningTerminal filamentThird larval instarEarly larval stagesMiR-317Ovarian somaMiR-14MiR-8Argonaute 1Mutant phenotypeDicer-1MiRNA pathwayPGC differentiationGerm lineGSC nicheRegulatory networks
2014
Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells
He J, Xuan T, Xin T, An H, Wang J, Zhao G, Li M. Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells. PLOS ONE 2014, 9: e103473. PMID: 25068272, PMCID: PMC4113433, DOI: 10.1371/journal.pone.0103473.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell Cycle ProteinsCell DifferentiationChromatin Assembly and DisassemblyChromosomal Proteins, Non-HistoneDrosophila melanogasterDrosophila ProteinsFemaleGene ExpressionGreen Fluorescent ProteinsMicroscopy, ConfocalMutationOvaryOvumProtein BindingReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTrans-ActivatorsTranscription FactorsConceptsGermline stem cellsGermline differentiationFate regulationDrosophila ovarian germline stem cellsSWI/SNF chromatin-remodeling complexOvarian germline stem cellsSWI/SNF complexStem cell fate regulationComplex-specific subunitsChromatin-remodeling complexCell fate regulationGenetic interaction testsStem cellsGSC fateGSC lossBAP complexDrosophila oogenesisMutant phenotypeSNF complexGSC maintenanceBRM functionRegulatory machineryEpigenetic regulationProtein complexesATPase subunits
2013
dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary
Xuan T, Xin T, He J, Tan J, Gao Y, Feng S, He L, Zhao G, Li M. dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Developmental Biology 2013, 379: 167-181. PMID: 23624310, DOI: 10.1016/j.ydbio.2013.04.015.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosis Regulatory ProteinsCell DifferentiationDNA PrimersDrosophila melanogasterDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHistonesMethylationMicroscopy, FluorescenceOvaryReal-Time Polymerase Chain ReactionRNA InterferenceStatistics, NonparametricStem Cell NicheStem CellsUbiquitin-Protein LigasesConceptsGermline stem cellsGerm cell differentiationStem cell nicheCell differentiationDrosophila ovaryGSC maintenanceDrosophila ovarian germline stem cellsCell nicheGermline stem cell maintenanceOvarian germline stem cellsCell fate regulationStem cell maintenanceHistone H3K4 trimethylationE3 ubiquitin ligaseStem cellsSingle germ cellEscort cellsGSC lossGSC regulationChromatin remodelingMutant ovariesH3K4 methylationHistone modificationsFate regulationH3K4 trimethylation
2009
Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis
Li Q, Shen L, Xin T, Xiang W, Chen W, Gao Y, Zhu M, Yu L, Li M. Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis. BMC Developmental Biology 2009, 9: 60. PMID: 19948068, PMCID: PMC2810132, DOI: 10.1186/1471-213x-9-60.Peer-Reviewed Original ResearchConceptsTumor suppressor geneDlg functionFate inductionPosterior patterningCell fateEgg chambersDrosophila tumor suppressor geneSuppressor geneBorder cell fateFollicle cellsCell fate inductionAnterior-posterior patterningDrosophila egg developmentRole of ScribFollicle cell epitheliumFurther genetic analysisMultiple signaling pathwaysDistinct cell typesAnterior-posterior axisDrosophila oogenesisEpithelial polarityEpithelial patterningMosaic clonesFollicular epitheliumAnterior domain