2016
MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis
Yang H, Li M, Hu X, Xin T, Zhang S, Zhao G, Xuan T, Li M. MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis. Developmental Biology 2016, 416: 312-323. PMID: 27339292, DOI: 10.1016/j.ydbio.2016.06.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryonic Germ CellsFemaleGene Expression Regulation, DevelopmentalGene Knockdown TechniquesLarvaLuminescent ProteinsMicroRNAsMicroscopy, FluorescenceOrganogenesisOvaryRibonuclease IIIRNA InterferenceRNA-Binding ProteinsStem Cell NicheConceptsOvary morphogenesisPrimordial germ cellsLate third larval instarLoss of DroshaMiRNA pathway componentsCanonical miRNA pathwayGerm cell lineageMiRNA-mediated regulationGerm cell precursorsGenome-wide screeningTerminal filamentThird larval instarEarly larval stagesMiR-317Ovarian somaMiR-14MiR-8Argonaute 1Mutant phenotypeDicer-1MiRNA pathwayPGC differentiationGerm lineGSC nicheRegulatory networks
2014
Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells
He J, Xuan T, Xin T, An H, Wang J, Zhao G, Li M. Evidence for Chromatin-Remodeling Complex PBAP-Controlled Maintenance of the Drosophila Ovarian Germline Stem Cells. PLOS ONE 2014, 9: e103473. PMID: 25068272, PMCID: PMC4113433, DOI: 10.1371/journal.pone.0103473.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell Cycle ProteinsCell DifferentiationChromatin Assembly and DisassemblyChromosomal Proteins, Non-HistoneDrosophila melanogasterDrosophila ProteinsFemaleGene ExpressionGreen Fluorescent ProteinsMicroscopy, ConfocalMutationOvaryOvumProtein BindingReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTrans-ActivatorsTranscription FactorsConceptsGermline stem cellsGermline differentiationFate regulationDrosophila ovarian germline stem cellsSWI/SNF chromatin-remodeling complexOvarian germline stem cellsSWI/SNF complexStem cell fate regulationComplex-specific subunitsChromatin-remodeling complexCell fate regulationGenetic interaction testsStem cellsGSC fateGSC lossBAP complexDrosophila oogenesisMutant phenotypeSNF complexGSC maintenanceBRM functionRegulatory machineryEpigenetic regulationProtein complexesATPase subunits
2013
The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche
Xin T, Xuan T, Tan J, Li M, Zhao G, Li M. The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche. Developmental Biology 2013, 384: 1-12. PMID: 24120347, DOI: 10.1016/j.ydbio.2013.10.001.Peer-Reviewed Original ResearchConceptsGermline stem cellsGSC maintenanceFate regulationEpigenetic mechanismsDrosophila ovarian germline stem cellsStem cellsOvarian germline stem cellsFemale germline stem cellsStem cell fate regulationCell fate regulationRNA binding proteinCell-autonomous roleAdult stem cellsGSC lossPutative histoneEnokEpigenetic regulationNiche maintenanceDaughter cellsHistone acetyltransferaseMaintenance defectsEctopic expressionMutant allelesBinding proteinMolecular studiesdBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary
Xuan T, Xin T, He J, Tan J, Gao Y, Feng S, He L, Zhao G, Li M. dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Developmental Biology 2013, 379: 167-181. PMID: 23624310, DOI: 10.1016/j.ydbio.2013.04.015.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosis Regulatory ProteinsCell DifferentiationDNA PrimersDrosophila melanogasterDrosophila ProteinsEpigenesis, GeneticFemaleGerm CellsHistone MethyltransferasesHistone-Lysine N-MethyltransferaseHistonesMethylationMicroscopy, FluorescenceOvaryReal-Time Polymerase Chain ReactionRNA InterferenceStatistics, NonparametricStem Cell NicheStem CellsUbiquitin-Protein LigasesConceptsGermline stem cellsGerm cell differentiationStem cell nicheCell differentiationDrosophila ovaryGSC maintenanceDrosophila ovarian germline stem cellsCell nicheGermline stem cell maintenanceOvarian germline stem cellsCell fate regulationStem cell maintenanceHistone H3K4 trimethylationE3 ubiquitin ligaseStem cellsSingle germ cellEscort cellsGSC lossGSC regulationChromatin remodelingMutant ovariesH3K4 methylationHistone modificationsFate regulationH3K4 trimethylation
2010
Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs
Zhu M, Xin T, Weng S, Gao Y, Zhang Y, Li Q, Li M. Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs. Cell Research 2010, 20: 242-245. PMID: 20066009, DOI: 10.1038/cr.2010.2.Peer-Reviewed Original Research
2009
Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis
Li Q, Shen L, Xin T, Xiang W, Chen W, Gao Y, Zhu M, Yu L, Li M. Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis. BMC Developmental Biology 2009, 9: 60. PMID: 19948068, PMCID: PMC2810132, DOI: 10.1186/1471-213x-9-60.Peer-Reviewed Original ResearchConceptsTumor suppressor geneDlg functionFate inductionPosterior patterningCell fateEgg chambersDrosophila tumor suppressor geneSuppressor geneBorder cell fateFollicle cellsCell fate inductionAnterior-posterior patterningDrosophila egg developmentRole of ScribFollicle cell epitheliumFurther genetic analysisMultiple signaling pathwaysDistinct cell typesAnterior-posterior axisDrosophila oogenesisEpithelial polarityEpithelial patterningMosaic clonesFollicular epitheliumAnterior domain
2008
Lethal(2)giant larvae is required in the follicle cells for formation of the initial AP asymmetry and the oocyte polarity during Drosophila oogenesis
Li Q, Xin T, Chen W, Zhu M, Li M. Lethal(2)giant larvae is required in the follicle cells for formation of the initial AP asymmetry and the oocyte polarity during Drosophila oogenesis. Cell Research 2008, 18: 372-384. PMID: 18268543, DOI: 10.1038/cr.2008.25.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell PolarityDrosophila melanogasterDrosophila ProteinsFemaleMaleMutationOocytesOogenesisOvarian FollicleTumor Suppressor ProteinsConceptsPosterior follicle cellsFollicle cellsOocyte polarityStalk cellsDrosophila tumor suppressor geneFLP/FRT systemSomatic follicle cellsDrosophila egg developmentTumor suppressor geneDrosophila oogenesisDV axesEgg chambersLgl functionEarly oogenesisFRT systemDorsoventral axesCell lineagesPosterior cellsAnteroposterior asymmetryRegulated differentiationSuppressor geneFunction mutationsPosterior localizationEgg developmentSpecific functions