The mTORC1/mTORC2 inhibitor AZD2014 enhances the radiosensitivity of glioblastoma stem-like cells
Kahn J, Hayman T, Jamal M, Rath B, Kramp T, Camphausen K, Tofilon P. The mTORC1/mTORC2 inhibitor AZD2014 enhances the radiosensitivity of glioblastoma stem-like cells. Neuro-Oncology 2013, 16: 29-37. PMID: 24311635, PMCID: PMC3870843, DOI: 10.1093/neuonc/not139.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBenzamidesBrain NeoplasmsCell CycleCell ProliferationDNA Breaks, Double-StrandedDNA RepairFemaleFluorescent Antibody TechniqueGlioblastomaHistonesHumansMechanistic Target of Rapamycin Complex 1Mechanistic Target of Rapamycin Complex 2MiceMice, NudeMorpholinesMultiprotein ComplexesNeoplastic Stem CellsProtein Kinase InhibitorsPyrimidinesRadiation-Sensitizing AgentsTOR Serine-Threonine KinasesTumor Cells, CulturedX-Ray TherapyXenograft Model Antitumor AssaysConceptsGBM stem-like cellsDual mTORC1/2 inhibitorStem-like cellsOrthotopic xenograftsMTORC1/2 inhibitorsPrimary treatment modalityΓH2AX fociRadiation-induced γH2AX fociGlioblastoma stem-like cellsTreatment modalitiesClonogenic survival analysisGBM therapyMTOR inhibitorsSurvival analysisGSC cell linesClonogenic assayMammalian targetVivo responseAZD2014GlioblastomaIndividual treatmentRadiosensitivityCell linesXenograftsImmunoblot analysis