2024
A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts
Johnson B, Iuliano M, Lam T, Biederer T, De Camilli P. A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts. Journal Of Cell Biology 2024, 223: e202311137. PMID: 39158698, PMCID: PMC11334333, DOI: 10.1083/jcb.202311137.Peer-Reviewed Original ResearchConceptsPlasma membraneNon-vesicular lipid transferSites of cell contactC-terminus motifsCell contact-dependent signalsContact-dependent signalingCell-cell contactER/PM junctionsTMEM24ER proteinsPM proteinsSynCAM 1Cell adhesion moleculesCellular functionsLipid transferC2CD2Phospholipid transportLipid transportCell contactProteinAdhesion moleculesCalcium homeostasisCellsFamily membersParalogsTransient impairment in microglial function causes sex-specific deficits in synaptic maturity and hippocampal function in mice exposed to early adversity
Ahmed S, Polis B, Jamwal S, Sanganahalli B, MacDowell Kaswan Z, Islam R, Kim D, Bowers C, Giuliano L, Biederer T, Hyder F, Kaffman A. Transient impairment in microglial function causes sex-specific deficits in synaptic maturity and hippocampal function in mice exposed to early adversity. Brain Behavior And Immunity 2024, 122: 95-109. PMID: 39134183, PMCID: PMC11402597, DOI: 10.1016/j.bbi.2024.08.010.Peer-Reviewed Original ResearchEarly-life adversityModel of early-life adversityContextual fear conditioningLimited beddingFear conditioningSynaptic engulfmentLB miceSynaptic pruningSex-specific deficitsHippocampus of maleSex-specific effectsHippocampal deficitsAdolescent miceHippocampal functionEarly adversityChemogenetic activationSynaptic connectionsBehavioral aberrationsPotential compensatory mechanismsSynaptic abnormalitiesHippocampusAblation of microgliaRodent hippocampusDeficitsWeeks of life
2023
The human milk component myo-inositol promotes neuronal connectivity
Paquette A, Carbone B, Vogel S, Israel E, Maria S, Patil N, Sah S, Chowdhury D, Kondratiuk I, Labhart B, Morrow A, Phillips S, Kuang C, Hondmann D, Pandey N, Biederer T. The human milk component myo-inositol promotes neuronal connectivity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2221413120. PMID: 37433002, PMCID: PMC10374161, DOI: 10.1073/pnas.2221413120.Peer-Reviewed Original ResearchConceptsHuman milkNeuronal connectionsInfant brainBreast milk componentsExcitatory synapse densityCultured rat neuronsMature brain tissueHuman milk samplesAbility of neuronsHuman excitatory neuronsDose-dependent mannerExcitatory postsynaptic sitesSlice culture systemSynapse densityExcitatory neuronsDietary supplementationPostsynaptic sitesRat neuronsNeuronal connectivityOrganotypic slicesBrain tissuePostsynaptic specializationsOrganotypic slice culture systemBrain developmentBrain connectivityConcerted roles of LRRTM1 and SynCAM 1 in organizing prefrontal cortex synapses and cognitive functions
de Arce K, Ribic A, Chowdhury D, Watters K, Thompson G, Sanganahalli B, Lippard E, Rohlmann A, Strittmatter S, Missler M, Hyder F, Biederer T. Concerted roles of LRRTM1 and SynCAM 1 in organizing prefrontal cortex synapses and cognitive functions. Nature Communications 2023, 14: 459. PMID: 36709330, PMCID: PMC9884278, DOI: 10.1038/s41467-023-36042-w.Peer-Reviewed Original ResearchConceptsPrefrontal cortexDKO miceSynCAM 1Aberrant neuronal activityDendritic spine numberPrefrontal cortex synapsesSynapse organizersSynapse numberMature brainNeuronal activityKnockout miceSpine numberSynapse developmentCognitive functionTrans-synaptic complexesImmunoglobulin family membersMiceFamily membersSynapsesLRRTM1Behavioral domainsHippocampusCognitive tasksConcerted roleCortex
2021
Subsynaptic positioning of AMPARs by LRRTM2 controls synaptic strength
Ramsey AM, Tang AH, LeGates TA, Gou XZ, Carbone BE, Thompson SM, Biederer T, Blanpied TA. Subsynaptic positioning of AMPARs by LRRTM2 controls synaptic strength. Science Advances 2021, 7: eabf3126. PMID: 34417170, PMCID: PMC8378824, DOI: 10.1126/sciadv.abf3126.Peer-Reviewed Original ResearchAMPA receptorsSynaptic strengthAdhesion moleculesPostsynaptic receptor activationTranscellular interactionsGlutamate releasePresynaptic sitesGlutamate receptorsReceptor numberSubsynaptic distributionReceptor activationSynaptic functionAMPAR numberSignificant deficitsRecent evidenceReceptorsSynapsesRapid remodelingExtracellular domainLRRTM2Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution
Hao X, Allgeyer ES, Lee DR, Antonello J, Watters K, Gerdes JA, Schroeder LK, Bottanelli F, Zhao J, Kidd P, Lessard MD, Rothman JE, Cooley L, Biederer T, Booth MJ, Bewersdorf J. Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution. Nature Methods 2021, 18: 688-693. PMID: 34059828, PMCID: PMC7610943, DOI: 10.1038/s41592-021-01149-9.Peer-Reviewed Original ResearchConceptsAdaptive optics schemeBest possible spatial resolutionPossible spatial resolutionOptical nanoscopyOptics schemeDiffraction limitDark stateOptical aberrationsNanoscopy methodsThin samplesThick specimenFluorescent moleculesSpatial resolutionThick specimensNanoscopeIsotropic resolutionResolutionNanoscopyPath geometryFluorescence microscopyMicroscopeMicroscopySynaptic recognition molecules in development and disease
Chowdhury D, Watters K, Biederer T. Synaptic recognition molecules in development and disease. Current Topics In Developmental Biology 2021, 142: 319-370. PMID: 33706921, PMCID: PMC8632550, DOI: 10.1016/bs.ctdb.2020.12.009.ChaptersConceptsSingle-cell expression studiesRecognition moleculesRecognition factorsKey protein familiesPost-translational modificationsLeucine-rich repeatsCell expression studiesSemaphorin/PlexinAlternative splicingProtein familyProteomic approachCombinatorial actionMolecular playersPartner recognitionExpression studiesMolecular themesSpecific expressionDisease relevanceSynapse specificationVertebrate brainImmunoglobulin SuperfamilyRich repertoireAppropriate brain regionsNeuron type-specific expressionSynaptic wiring
2019
SynGO: An Evidence-Based, Expert-Curated Knowledge Base for the Synapse
Koopmans F, van Nierop P, Andres-Alonso M, Byrnes A, Cijsouw T, Coba M, Cornelisse L, Farrell R, Goldschmidt H, Howrigan D, Hussain N, Imig C, de Jong A, Jung H, Kohansalnodehi M, Kramarz B, Lipstein N, Lovering R, MacGillavry H, Mariano V, Mi H, Ninov M, Osumi-Sutherland D, Pielot R, Smalla K, Tang H, Tashman K, Toonen R, Verpelli C, Reig-Viader R, Watanabe K, van Weering J, Achsel T, Ashrafi G, Asi N, Brown T, De Camilli P, Feuermann M, Foulger R, Gaudet P, Joglekar A, Kanellopoulos A, Malenka R, Nicoll R, Pulido C, de Juan-Sanz J, Sheng M, Südhof T, Tilgner H, Bagni C, Bayés À, Biederer T, Brose N, Chua J, Dieterich D, Gundelfinger E, Hoogenraad C, Huganir R, Jahn R, Kaeser P, Kim E, Kreutz M, McPherson P, Neale B, O'Connor V, Posthuma D, Ryan T, Sala C, Feng G, Hyman S, Thomas P, Smit A, Verhage M. SynGO: An Evidence-Based, Expert-Curated Knowledge Base for the Synapse. Neuron 2019, 103: 217-234.e4. PMID: 31171447, PMCID: PMC6764089, DOI: 10.1016/j.neuron.2019.05.002.Peer-Reviewed Original ResearchSynaptic Connectivity and Cortical Maturation Are Promoted by the ω-3 Fatty Acid Docosahexaenoic Acid
Carbone BE, Abouleish M, Watters KE, Vogel S, Ribic A, Schroeder OH, Bader BM, Biederer T. Synaptic Connectivity and Cortical Maturation Are Promoted by the ω-3 Fatty Acid Docosahexaenoic Acid. Cerebral Cortex 2019, 30: 226-240. PMID: 31034037, DOI: 10.1093/cercor/bhz083.Peer-Reviewed Original ResearchConceptsVisual acuityDietary DHASynaptic connectivityFatty Acid Docosahexaenoic AcidVivo electrophysiological recordingsSize of synapsesEarly neuronal differentiationDose-dependent mannerFatty acid DHACortical maturationYoung miceAwake miceDendritic arborsCultured neuronsDHA's roleVisual cortexFunctional maturationPostsynaptic specializationsElectrophysiological recordingsCortical processingBrain developmentDocosahexaenoic acidAcid DHAPostnatal stagesNeuronal differentiationSynapse-Selective Control of Cortical Maturation and Plasticity by Parvalbumin-Autonomous Action of SynCAM 1
Ribic A, Crair MC, Biederer T. Synapse-Selective Control of Cortical Maturation and Plasticity by Parvalbumin-Autonomous Action of SynCAM 1. Cell Reports 2019, 26: 381-393.e6. PMID: 30625321, PMCID: PMC6345548, DOI: 10.1016/j.celrep.2018.12.069.Peer-Reviewed Original ResearchConceptsCortical plasticityCell adhesion molecule-1Critical periodJuvenile-like plasticityAdhesion molecule-1Primary visual cortexVisual critical periodThalamocortical inputsCortical maturationCircuit maturationV1 plasticityParvalbumin interneuronsFeedforward inhibitionSynaptic cell adhesion molecule 1Cell-autonomous mechanismsBrief lossCortical responsesSynaptic lociMolecule-1Visual cortexSynaptic factorsInterneuronsSpecific knockdownAdulthoodEyes
2018
Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins
Cijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T. Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins. Proteomes 2018, 6: 48. PMID: 30487426, PMCID: PMC6313906, DOI: 10.3390/proteomes6040048.Peer-Reviewed Original ResearchProximity labelingLabel-free quantitation mass spectrometryReceptor-type tyrosine-protein phosphatase zetaProximity labeling approachCell-cell contactSynaptic cleftPost-synaptic specializationsProteomic contentSynapse heterogeneitySynCAM 1Confocal microcopySurface proteinsCell surfaceProteomeSuper-resolution imagingCultured cortical neuronsMammalian brainLabeling approachMolecular compositionReporterProteinCortical neuronsFunctional organizationExcitatory synapsesDiverse set
2017
Transcellular Nanoalignment of Synaptic Function
Biederer T, Kaeser PS, Blanpied TA. Transcellular Nanoalignment of Synaptic Function. Neuron 2017, 96: 680-696. PMID: 29096080, PMCID: PMC5777221, DOI: 10.1016/j.neuron.2017.10.006.Peer-Reviewed Original Research
2016
Excitatory Synaptic Drive and Feedforward Inhibition in the Hippocampal CA3 Circuit Are Regulated by SynCAM 1
Park KA, Ribic A, Gaupp F, Coman D, Huang Y, Dulla CG, Hyder F, Biederer T. Excitatory Synaptic Drive and Feedforward Inhibition in the Hippocampal CA3 Circuit Are Regulated by SynCAM 1. Journal Of Neuroscience 2016, 36: 7464-7475. PMID: 27413156, PMCID: PMC4945666, DOI: 10.1523/jneurosci.0189-16.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCA3 Region, HippocampalCell Adhesion Molecule-1Cell Adhesion MoleculesConditioning, ClassicalFearFemaleGABA AntagonistsGene Expression RegulationImmunoglobulinsIn Vitro TechniquesMaleMemory DisordersMiceMice, Inbred C57BLMice, KnockoutNeural InhibitionNeural PathwaysParvalbuminsPyridazinesSynapsesSynaptic PotentialsTime FactorsConceptsDiffusion tensor imagingHippocampal CA3 areaKO miceMagnetic resonance imagingExcitatory inputsFeedforward inhibitionSynCAM 1Pyramidal neuronsNetwork excitabilityCA3 areaMossy fibersExcitatory mossy fiber inputBrain regionsTensor imagingHippocampal CA3 circuitsParvalbumin-positive interneuronsCA3 pyramidal neuronsPV-positive interneuronsExcitatory synaptic driveKnock-out (KO) miceDevelopment of synapsesDifferent neuronal populationsMossy fiber inputsDistinct brain regionsSynaptic imbalance
2015
Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains
de Arce K, Schrod N, Metzbower SWR, Allgeyer E, Kong G, Tang AH, Krupp AJ, Stein V, Liu X, Bewersdorf J, Blanpied TA, Lucić V, Biederer T. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains. Neuron 2015, 88: 1165-1172. PMID: 26687224, PMCID: PMC4687029, DOI: 10.1016/j.neuron.2015.11.011.Peer-Reviewed Original ResearchConceptsSynaptic cell adhesion molecule 1Trans-synaptic complexesEphB2 receptor tyrosine kinaseReceptor tyrosine kinasesCryo-ETSynaptic cleftCryoelectron tomographyTyrosine kinaseMolecular insightsSynCAM 1Macromolecular organizationImmunoglobulin proteinCell adhesion molecule-1Immunoelectron microscopyAdhesion molecule-1Super-resolution imagingPostsynaptic densityDistinct density profilesDepression paradigmExcitatory synapsesPostsynaptic areaMolecule-1Cleft edgesSynapsesCleftKey Molecules: SynCAM Proteins☆
Biederer T, Shrestha N. Key Molecules: SynCAM Proteins☆. 2015 DOI: 10.1016/b978-0-12-801238-3.04784-x.Peer-Reviewed Original ResearchSynaptic cell adhesion moleculesPostsynaptic neurotransmitter receptorsPresynaptic release machineryCell adhesion moleculeAdhesion proteinsPost-synaptic specializationsInstructive signalsContact sitesSynaptic contact sitesRelease machinerySynapse inductionCentral nervous systemAdhesion moleculesNeurotransmitter receptorsProteinSynaptic specializationsNervous systemAsymmetric sitesSpecializationMachinerySitesConcomitant formationAssemblyInductionFamily
2014
Activity-Dependent Regulation of Dendritic Complexity by Semaphorin 3A through Farp1
Cheadle L, Biederer T. Activity-Dependent Regulation of Dendritic Complexity by Semaphorin 3A through Farp1. Journal Of Neuroscience 2014, 34: 7999-8009. PMID: 24899721, PMCID: PMC4044256, DOI: 10.1523/jneurosci.3950-13.2014.Peer-Reviewed Original ResearchConceptsDendritic complexityTotal dendritic branch lengthActivity-dependent regulationDendritic shaftsDendritic arborizationDendritic arborsHippocampal neuronsSynaptic inputsNeuronal activityRat neuronsSemaphorin 3ANeuronal structuresSema3ADendrite differentiationNeuronsRac1 activatorDendritic morphologyComplex neuronal structuresPlexinA1Soluble cuesSignaling proteinsArborizationFARP1CoreceptorStructural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1
Ribic A, Liu X, Crair MC, Biederer T. Structural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1. The Journal Of Comparative Neurology 2014, 522: 900-920. PMID: 23982969, PMCID: PMC3947154, DOI: 10.1002/cne.23452.Peer-Reviewed Original ResearchMeSH KeywordsAlcohol OxidoreductasesAnalysis of VarianceAnimalsAnimals, NewbornCell Adhesion Molecule-1Cell Adhesion MoleculesCo-Repressor ProteinsDNA-Binding ProteinsElectroretinographyFemaleGene Expression Regulation, DevelopmentalImmunoglobulinsMaleMiceMice, Inbred C57BLMice, KnockoutMicroscopy, ImmunoelectronNerve Tissue ProteinsPhosphoproteinsReceptors, Metabotropic GlutamateRetinaRetinal Rod Photoreceptor CellsSynapsesVesicular Glutamate Transport Protein 1ConceptsCell adhesion molecule-1Adhesion molecule-1Ribbon synapsesKO retinasSynaptic cell adhesion molecule 1Molecule-1Mouse photoreceptor ribbon synapsesInner retinal layersPhotoreceptor ribbon synapsesRod visual pathwayEarly postnatal stagesPlexiform layerKO micePhotoreceptor synapsesSynaptic organizationExcitatory synapsesQuantitative ultrastructural analysisRetinal layersKnockout miceOuter nuclearVisual pathwaySynapse developmentElectroretinogram recordingsPostnatal stagesAdhesion molecules
2012
The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization
Cheadle L, Biederer T. The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. Journal Of Cell Biology 2012, 199: 985-1001. PMID: 23209303, PMCID: PMC3518221, DOI: 10.1083/jcb.201205041.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell Adhesion Molecule-1Cell Adhesion MoleculesCytoskeletal ProteinsCytoskeletonDendritic SpinesGuanine Nucleotide Exchange FactorsHEK293 CellsHippocampusHumansImmunoglobulinsMiceMice, KnockoutNeurogenesisNeuronsNeuropeptidesProtein BindingProtein Structure, TertiaryProteomicsRac GTP-Binding ProteinsRac1 GTP-Binding ProteinRho Guanine Nucleotide Exchange FactorsSignal TransductionSynapsesConceptsSynCAM 1Synapse developmentF-actin assemblyCytoskeletal dynamicsGTPase Rac1Retrograde signalsSynaptic adhesionFARP1Transsynaptic interactionsFilopodial dynamicsProtein 1Synapse formationSynaptic complexImmature neuronsSpine densitySpine morphologySynapse numberPathwayKnockout miceSynaptic membranesPleckstrinFERMRac1The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior
Giza JI, Jung Y, Jeffrey RA, Neugebauer NM, Picciotto MR, Biederer T. The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior. Neuropsychopharmacology 2012, 38: 628-638. PMID: 23169347, PMCID: PMC3572459, DOI: 10.1038/npp.2012.226.Peer-Reviewed Original ResearchConceptsNucleus accumbensDrugs of abuseSynCAM 1Cocaine effectsSynapse structureMushroom-type spinesExcitatory synapse numberMedium spiny neuronsAddiction-related behaviorsKO miceSpiny neuronsStubby spinesSynapse numberAdhesion molecule proteinsExcitatory synapsesCocaine administrationTrans-synaptic interactionsKnockout micePsychostimulant effectsNAc synapsesStructural remodelingTherapeutic interventionsSynaptic cleftPsychostimulant cocaineSynapse developmentSpecific N‐glycans on SynCAM Ig proteins regulate synaptic adhesion and synapse development
Biederer T. Specific N‐glycans on SynCAM Ig proteins regulate synaptic adhesion and synapse development. The FASEB Journal 2012, 26: 232.2-232.2. DOI: 10.1096/fasebj.26.1_supplement.232.2.Peer-Reviewed Original ResearchTrans-synaptic interactionsSynapse developmentN-glycansSite-specific N-glycosylationSpecialized cell junctionsSpecific N-glycansProtein complexesFirst Ig domainN-glycosylationBinding interfaceSynaptic adhesionIg domainsFunctional analysisSynCAM 1Cell junctionsIg1 domainImmunoglobulin proteinNovel mechanismIg proteinGlycosylationProteinAdhesive interactionsSynCAMSynaptic cleftExcitatory synapses