2024
Signal integration and adaptive sensory diversity tuning in Escherichia coli chemotaxis
Moore J, Kamino K, Kottou R, Shimizu T, Emonet T. Signal integration and adaptive sensory diversity tuning in Escherichia coli chemotaxis. Cell Systems 2024, 15: 628-638.e8. PMID: 38981486, PMCID: PMC11307269, DOI: 10.1016/j.cels.2024.06.003.Peer-Reviewed Original ResearchEscherichia coli chemotaxisPopulation of E. coliMeasure kinase activityPhenotypic diversitySensory diversityDecreased diversityKinase activityDiverse phenotypesChemical signalsDiversitySingle cellsResponse to changesCellsSignalPhenotypePopulationChemotaxisSignal integrityLigandCompetitive ligandChemoattractant
2021
Non-Genetic Diversity in Chemosensing and Chemotactic Behavior
Moore JP, Kamino K, Emonet T. Non-Genetic Diversity in Chemosensing and Chemotactic Behavior. International Journal Of Molecular Sciences 2021, 22: 6960. PMID: 34203411, PMCID: PMC8268644, DOI: 10.3390/ijms22136960.Peer-Reviewed Original Research
2005
Real-time RNA profiling within a single bacterium
Le TT, Harlepp S, Guet C, Dittmar K, Emonet T, Pan T, Cluzel P. Real-time RNA profiling within a single bacterium. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 9160-9164. PMID: 15967986, PMCID: PMC1166617, DOI: 10.1073/pnas.0503311102.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell CycleCell ProliferationDose-Response Relationship, DrugDrug Resistance, MultipleEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterGenotypeGreen Fluorescent ProteinsModels, StatisticalPlasmidsPromoter Regions, GeneticProtein BindingRecombinant Fusion ProteinsRNARNA, MessengerSpectrometry, FluorescenceSpliceosomesTime FactorsTranscription, GeneticConceptsRNA profilingTranscriptional dynamicsInducible gene expression systemGene expression systemSingle cellsSpecific RNA levelsSingle bacteriumRNA levelsGenetic systemSpecific promotersFluorescence correlation spectroscopyExpression systemEscherichia coliEfflux pump systemGenetic deletionProfilingBacteriumPopulation measurementsKey determinantAntibiotic resistanceProkaryotesCellsPromoterCorrelation spectroscopyRNAAgentCell: a digital single-cell assay for bacterial chemotaxis
Emonet T, Macal CM, North MJ, Wickersham CE, Cluzel P. AgentCell: a digital single-cell assay for bacterial chemotaxis. Bioinformatics 2005, 21: 2714-2721. PMID: 15774553, DOI: 10.1093/bioinformatics/bti391.Peer-Reviewed Original ResearchConceptsBacterial chemotaxisSingle-cell biologySingle-cell assaysSingle-cell levelChemotaxis networkCellular behaviorBacterial populationsIntracellular processesIndividual cellsSingle cellsSwimming cellsMolecular interactionsChemotaxis assaysBiological systemsNew computational approachCellsComputational approachChemotaxisAssaysFlagellaBiologyBacterium