2024
Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula–interpeduncular nucleus pathway in mice
Tsuzuki A, Yamasaki M, Konno K, Miyazaki T, Takei N, Tomita S, Yuzaki M, Watanabe M. Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula–interpeduncular nucleus pathway in mice. Scientific Reports 2024, 14: 14193. PMID: 38902419, PMCID: PMC11189931, DOI: 10.1038/s41598-024-65076-3.Peer-Reviewed Original ResearchConceptsMHb-IPN pathwayMHb neuronsNicotine dependenceNicotinic acetylcholine receptorsAcetylcholine receptorsNicotine-related behaviorsCell surfaceImmunoelectron microscopySubunitAxonal compartmentFunctional roleNeurotransmitter releasePresynaptic terminalsSubcellular expressionPathwaySimultaneous detectionDistribution patternsSynaptic junctionsNAChRsAnatomical basisExpressionNegative controlReceptorsNeuronsAntibodies
2019
Zebrafish behavioural profiling identifies GABA and serotonin receptor ligands related to sedation and paradoxical excitation
McCarroll MN, Gendelev L, Kinser R, Taylor J, Bruni G, Myers-Turnbull D, Helsell C, Carbajal A, Rinaldi C, Kang HJ, Gong JH, Sello JK, Tomita S, Peterson RT, Keiser MJ, Kokel D. Zebrafish behavioural profiling identifies GABA and serotonin receptor ligands related to sedation and paradoxical excitation. Nature Communications 2019, 10: 4078. PMID: 31501447, PMCID: PMC6733874, DOI: 10.1038/s41467-019-11936-w.Peer-Reviewed Original ResearchConceptsParadoxical excitationGABAA receptorsCentral nervous system depressantsSerotonin 6 receptorMost anesthetic drugsDifferent neuronal targetsHuman GABAA receptorsNeuronal targetsNeuronal activityAnesthetic drugsMotor activitySerotonin receptor ligandsSedationReceptor ligandsReceptorsCaudal hindbrainAnestheticsPrimary targetPrevious studiesGABATargetNeuronsActivityBrain
2013
Cornichons Control ER Export of AMPA Receptors to Regulate Synaptic Excitability
Brockie PJ, Jensen M, Mellem JE, Jensen E, Yamasaki T, Wang R, Maxfield D, Thacker C, Hoerndli F, Dunn PJ, Tomita S, Madsen DM, Maricq AV. Cornichons Control ER Export of AMPA Receptors to Regulate Synaptic Excitability. Neuron 2013, 80: 129-142. PMID: 24094107, PMCID: PMC3795439, DOI: 10.1016/j.neuron.2013.07.028.Peer-Reviewed Original ResearchConceptsGlutamatergic synaptic transmissionGlutamate-gated currentsNervous system functionIonotropic glutamate receptorsC. elegansER exportSynaptic excitabilityCargo adaptorsTransgenic wormsGenetic approachesOpposite phenotypeCornichon ProteinsGlutamate receptorsSynaptic transmissionAgonist AMPAHeterologous cellsAMPA receptorsCentral synapsesAMPAR numberSynaptic communicationReconstitution studies
2011
Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1
Straub C, Hunt DL, Yamasaki M, Kim KS, Watanabe M, Castillo PE, Tomita S. Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1. Nature Neuroscience 2011, 14: 866-873. PMID: 21623363, PMCID: PMC3125417, DOI: 10.1038/nn.2837.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysical PhenomenaBiophysicsCA1 Region, HippocampalCell Line, TransformedCerebellumDisks Large Homolog 4 ProteinDizocilpine MaleateDose-Response Relationship, DrugDrug InteractionsElectric StimulationExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGene Expression RegulationGreen Fluorescent ProteinsGuanylate KinasesHumansImmunoprecipitationIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsKainic AcidLDL-Receptor Related ProteinsLipoproteins, LDLMembrane PotentialsMembrane ProteinsMiceMice, KnockoutNeuronsPatch-Clamp TechniquesPresynaptic TerminalsProtein BindingProtein SubunitsReceptors, Kainic AcidReceptors, N-Methyl-D-AspartateSynaptophysinTransfectionTritium
2009
A Transmembrane Accessory Subunit that Modulates Kainate-Type Glutamate Receptors
Zhang W, St-Gelais F, Grabner CP, Trinidad JC, Sumioka A, Morimoto-Tomita M, Kim KS, Straub C, Burlingame AL, Howe JR, Tomita S. A Transmembrane Accessory Subunit that Modulates Kainate-Type Glutamate Receptors. Neuron 2009, 61: 385-396. PMID: 19217376, PMCID: PMC2803770, DOI: 10.1016/j.neuron.2008.12.014.Peer-Reviewed Original ResearchConceptsKainate-type glutamate receptorsGlutamate receptorsIonotropic glutamate receptorsKainate receptorsSynaptic transmissionSurface expressionNative kainate receptorsFast synaptic transmissionKainate receptor subunitsBrain-specific proteinsExcitatory transmissionNMDA receptorsAMPA receptorsReceptor subunitsReceptorsProtein levelsNETO2Auxiliary subunitsTARP auxiliary subunitsBrainVertebrate brainKainate receptor GluR6Proteomic screenMajor roleMEPSCs
2000
Regulation of X11L-dependent Amyloid Precursor Protein Metabolism by XB51, a Novel X11L-binding Protein*
Lee D, Tomita S, Kirino Y, Suzuki T. Regulation of X11L-dependent Amyloid Precursor Protein Metabolism by XB51, a Novel X11L-binding Protein*. Journal Of Biological Chemistry 2000, 275: 23134-23138. PMID: 10833507, DOI: 10.1074/jbc.c000302200.Peer-Reviewed Original ResearchPDZ Domain-dependent Suppression of NF-κB/p65-induced Aβ42 Production by a Neuron-specific X11-like Protein*
Tomita S, Fujita T, Kirino Y, Suzuki T. PDZ Domain-dependent Suppression of NF-κB/p65-induced Aβ42 Production by a Neuron-specific X11-like Protein*. Journal Of Biological Chemistry 2000, 275: 13056-13060. PMID: 10777610, DOI: 10.1074/jbc.c000019200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlzheimer DiseaseAmyloid beta-PeptidesAnimalsBrainCell LineCOS CellsDNA, ComplementaryGene Expression RegulationGene LibraryHumansLuciferasesNerve Tissue ProteinsNeuronsNF-kappa BNF-kappa B p50 SubunitPeptide FragmentsPrecipitin TestsProtein BindingProtein IsoformsProtein Structure, TertiaryTranscription Factor RelATranscription, GeneticTransfectionTwo-Hybrid System TechniquesConceptsNF-kappaB/p65X11-like proteinsAlzheimer's diseaseNF-kappaB/p50Progression of ADAmyloid precursor proteinSpecific therapyAbeta productionAβ42 productionAbeta42 productionNF-kappaBP65Neuronal cellsAmino acids 161Precursor proteinX11LAbeta42DiseaseRel homology domainSecretionX11L.P50LIN-10Transcription factorsTherapy
1999
Interaction of a Neuron-specific Protein Containing PDZ Domains with Alzheimer's Amyloid Precursor Protein*
Tomita S, Ozaki T, Taru H, Oguchi S, Takeda S, Yagi Y, Sakiyama S, Kirino Y, Suzuki T. Interaction of a Neuron-specific Protein Containing PDZ Domains with Alzheimer's Amyloid Precursor Protein*. Journal Of Biological Chemistry 1999, 274: 2243-2254. PMID: 9890987, DOI: 10.1074/jbc.274.4.2243.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlzheimer DiseaseAmino Acid SequenceAmyloid beta-Protein PrecursorAnimalsChromosome MappingChromosomes, Human, Pair 9Cloning, MolecularDNA, ComplementaryHumansMolecular Sequence DataNerve Tissue ProteinsNeuronsProtein BindingProtein Processing, Post-TranslationalSequence Homology, Amino Acid