2016
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNucleaseBox C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs
Yip WS, Shigematsu H, Taylor DW, Baserga SJ. Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs. Nucleic Acids Research 2016, 44: 8976-8989. PMID: 27342279, PMCID: PMC5062973, DOI: 10.1093/nar/gkw576.Peer-Reviewed Original ResearchConceptsBox C/DArchaeal box C/D sRNPsBox C/D sRNAsBox C/D sRNPRibosomal RNA modificationsElectron microscopy modelCryo-EM reconstructionCellular organismsRibosome functionRNA modificationsSRNAsSRNPModification typesStructural techniquesEukaryaPotential interactionsStemArchaeaStructural analysisStem endClose proximityOrganismsNuclear magnetic resonance studiesCatalyseInteraction
2001
An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association.
Wormsley S, Samarsky D, Fournier M, Baserga S. An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association. RNA 2001, 7: 904-19. PMID: 11421365, PMCID: PMC1370138, DOI: 10.1017/s1355838201010238.Peer-Reviewed Original ResearchConceptsU3 snoRNAU3 snoRNPYeast U3 snoRNAU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinSmall nucleolar RNAsStretch of nucleotidesHinge regionBase-pairing interactionsRRNA transcriptsNucleolar RNAsNucleolar ribonucleoproteinConserved elementSequence elementsProtein componentsSnoRNAsProcessing reactionsBase pairsMpp10pBase pairingSnoRNPsFunctional centersRRNACritical roleProtein
1992
Distinct molecular signals for nuclear import of the nucleolar snRNA, U3.
Baserga S, Gilmore-Hebert M, Yang X. Distinct molecular signals for nuclear import of the nucleolar snRNA, U3. Genes & Development 1992, 6: 1120-1130. PMID: 1592260, DOI: 10.1101/gad.6.6.1120.Peer-Reviewed Original ResearchConceptsCap trimethylationNuclear importU3 RNADistinct molecular signalsXenopus oocytesRegulation of importU3 geneCytoplasmic phaseNucleolar snRNAOptimal importU3 snRNAMolecular signalsSnRNAU3 snRNPNucleotidesTrimethylationCytoplasmAutoantigenic proteinsRNAArtificial constructsStemImportOocytesBiogenesisSnRNP