2023
Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing
McCool M, Bryant C, Huang H, Ogawa L, Farley-Barnes K, Sondalle S, Abriola L, Surovtseva Y, Baserga S. Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing. RNA Biology 2023, 20: 257-271. PMID: 37246770, PMCID: PMC10228412, DOI: 10.1080/15476286.2023.2217392.Peer-Reviewed Original ResearchConceptsPre-rRNA accumulationRibosome biogenesisNonessential roleEukaryotic ribosome biogenesisEssential cellular processesNucleolar stress responsePre-rRNA levelsRRNA processingLikely orthologCellular processesAssociated proteinsTumor suppressorStress responseHuman cellsProtein synthesisProtein 7Human counterpartBiogenesisYeastOrthologsHomologSubcomplexAccumulationRRNATranscription
2016
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNuclease
1999
Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Lee S, Baserga S. Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing. Molecular And Cellular Biology 1999, 19: 5441-5452. PMID: 10409734, PMCID: PMC84386, DOI: 10.1128/mcb.19.8.5441.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceGenes, ReporterGenetic TechniquesGenetic VectorsMacromolecular SubstancesMolecular Sequence DataPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA PrecursorsRNA, FungalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinProtein componentsU3 snoRNPNucleolar ribonucleoproteinGenetic depletionTwo-hybrid methodologyNovel protein componentsPre-rRNA processingUnique protein componentRibosomal proteinsSnoRNA levelsRRNA processingU3 snoRNARRNA precursorIMP proteinU3 RNAS4 familiesPrecise functionSevere defectsCleavage eventsMpp10pProteinSnoRNPsRNA
1998
The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes.
Dunbar D, Baserga S. The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes. RNA 1998, 4: 195-204. PMID: 9570319, PMCID: PMC1369608.Peer-Reviewed Original Research
1997
Mpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast
Dunbar D, Wormsley S, Agentis T, Baserga S. Mpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast. Molecular And Cellular Biology 1997, 17: 5803-5812. PMID: 9315638, PMCID: PMC232428, DOI: 10.1128/mcb.17.10.5803.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCloning, MolecularEscherichia coliGenes, FungalHumansMiceMolecular Sequence DataMolecular WeightPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, RibosomalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSpores, FungalConceptsYeast proteinsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinNovel protein componentsPre-rRNA processingSmall nucleolar ribonucleoproteinS. cerevisiae cellsSpecific protein componentsPulse-chase analysisMitotic phosphorylationRibosome biogenesisEssential genesMpp10pYeast SaccharomycesNovel proteinU3 snoRNARRNA precursorNucleolar ribonucleoproteinSites A0Tetrad analysisHuman proteinsNull allelesCerevisiae cellsGenBank searchMpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors.
Baserga S, Agentis T, Wormsley S, Dunbar D, Lee S. Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors. Nucleic Acids Symposium Series 1997, 64-7. PMID: 9478208.Peer-Reviewed Original ResearchConceptsPre-rRNA processingNew protein componentsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinEssential genesSlow growthConditional promoterU3 snoRNAMpp10pNucleolar ribonucleoproteinRRNA precursorProcessing eventsYeastSnoRNPsMpp10ProteinModel systemDegrees C. AnalysisC. AnalysisSnoRNAsMutantsRibonucleoproteinSaccharomyces