2002
A Comparison of Two Surface Sample Collection Devices for Use in Polymerase Chain Reaction Based Detection of Pneumocystis carinii in House Dust
Maher N, Dillon HK, Awooda A, Lee JH, Vermund SH, Unnasch TR. A Comparison of Two Surface Sample Collection Devices for Use in Polymerase Chain Reaction Based Detection of Pneumocystis carinii in House Dust. Applied Occupational And Environmental Hygiene 2002, 17: 416-423. PMID: 12049431, DOI: 10.1080/10473220290035435.Peer-Reviewed Original ResearchConceptsPolymerase Chain Reaction Based DetectionDNA detectionComposite dust samplesDetection assaysBest deviceP. carinii DNAPCR protocolSample collection deviceDevicesCassette methodDust collection efficiencyCollection efficiencyCarinii DNABased DetectionDust collection deviceOptimal assayFilter cassetteCollection deviceCassettePolymerase chain reactionVacuum pumpDetectionEfficiencyChain reactionProtocol
2001
Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA
Maher N, Dillon H, Vermund S, Unnasch T. Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA. Applied And Environmental Microbiology 2001, 67: 449-452. PMID: 11133478, PMCID: PMC92598, DOI: 10.1128/aem.67.1.449-452.2001.Peer-Reviewed Original Research
2000
Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii
Maher N, Vermund S, Lasbury M, Lee C, Bartlett M, Unnasch T. Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii. Journal Of Clinical Microbiology 2000, 38: 1947-1952. PMID: 10790126, PMCID: PMC86630, DOI: 10.1128/jcm.38.5.1947-1952.2000.Peer-Reviewed Original ResearchConceptsPneumocystis carinii pneumoniaRespiratory specimensP. cariniiViable trophozoitesPatient respiratory specimensP. carinii DNAOpportunistic illnessesViability assaysCarinii pneumoniaPCP patientsShock protein 70 familyReverse transcription-PCRHeat shock protein 70 familyPneumocystis cariniiNoninvasive techniqueCarinii DNACariniiTranscription-PCREthidium bromide stainingOptimal assay conditionsTrophozoitesBromide stainingAssaysMolecular viabilitySpecific primers
1997
Detection of Pneumocystis carinii DNA in air samples: likely environmental risk to susceptible persons
Bartlett MS, Vermund SH, Jacobs R, Durant PJ, Shaw MM, Smith JW, Tang X, Lu JJ, Li B, Jin S, Lee CH. Detection of Pneumocystis carinii DNA in air samples: likely environmental risk to susceptible persons. Journal Of Clinical Microbiology 1997, 35: 2511-2513. PMID: 9316898, PMCID: PMC230001, DOI: 10.1128/jcm.35.10.2511-2513.1997.Peer-Reviewed Original ResearchConceptsP. carinii DNAHospital roomCarinii DNAPatient's hospital roomPneumocystis carinii DNASusceptible personsControl homesPatient's homeInfectious organismsPatientsPneumocystis cariniiCariniiP. cariniiAirborne presenceSpecific environmental sourcesAir samplesPersonsEnvironmental sourcesHomeEnvironmental sampling