2022
High-content CRISPR screening
Bock C, Datlinger P, Chardon F, Coelho M, Dong M, Lawson K, Lu T, Maroc L, Norman T, Song B, Stanley G, Chen S, Garnett M, Li W, Moffat J, Qi L, Shapiro R, Shendure J, Weissman J, Zhuang X. High-content CRISPR screening. Nature Reviews Methods Primers 2022, 2: 8. DOI: 10.1038/s43586-021-00093-4.Peer-Reviewed Original ResearchCRISPR screeningCRISPR screensBiological discoverySingle-cell RNA sequencingPooled CRISPR screensList of genesHigh-content methodBiological challengesGene functionCell competitionUnbiased interrogationGuide RNARNA sequencingBioinformatics analysisDetailed biological insightsTarget genesBasic biologyPool of cellsBiological insightsCRISPR technologyMolecular mechanismsSuch screensGenesMedical GeneticsBroad utility
2011
Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster
Chen Y, Dai H, Chen S, Zhang L, Long M. Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster. PLOS ONE 2011, 6: e18853. PMID: 21541324, PMCID: PMC3082539, DOI: 10.1371/journal.pone.0018853.Peer-Reviewed Original ResearchMeSH KeywordsAnimal StructuresAnimalsBase PairingBase SequenceBrainConserved SequenceCourtshipDrosophila melanogasterFemaleGene Expression ProfilingGene Expression RegulationGenome, InsectGreen Fluorescent ProteinsMaleMolecular Sequence DataMutationOrgan SpecificityPeripheral Nervous SystemPromoter Regions, GeneticRNA, UntranslatedSexual Behavior, AnimalTransformation, GeneticConceptsDrosophila melanogasterBp regionCourtship behaviorNon-coding RNA genesWhole genome expression profilingBp upstream regionTissue-specific expressionGenome expression profilingMale courtship behaviorMale accessory glandsMelanogaster subgroupDrosophila speciesD. virilisRNA genesGene categoriesProtein functionKnockout mutationsWing hairsMale courtshipTarget genesExpression profilingGFP signalNegative regulatorEnhancer elementsExpression signals