2013
Real Time Measures of Prestin Charge and Fluorescence during Plasma Membrane Trafficking Reveal Sub-Tetrameric Activity
Bian S, Navaratnam D, Santos-Sacchi J. Real Time Measures of Prestin Charge and Fluorescence during Plasma Membrane Trafficking Reveal Sub-Tetrameric Activity. PLOS ONE 2013, 8: e66078. PMID: 23762468, PMCID: PMC3677934, DOI: 10.1371/journal.pone.0066078.Peer-Reviewed Original ResearchConceptsObligate tetramerPlasma membraneMembrane motor proteinIntegral membrane proteinsTetracycline-inducible cell lineMembrane proteinsMotor proteinsPrestin densityTemperature blockPrestinFluorescence measuresMembrane fluorescenceCell linesNonlinear capacitanceCochlear amplificationProteinTetramerMembraneFluorescencePrevious observationsVoltage clampFluorescence methodCellsAmplification
2012
Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels
Yan Y, Yang Y, Bian S, Sigworth FJ. Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels. The Journal Of Membrane Biology 2012, 245: 667-674. PMID: 22729647, PMCID: PMC3903048, DOI: 10.1007/s00232-012-9425-7.Peer-Reviewed Original ResearchMeSH KeywordsCell LineGene ExpressionHEK293 CellsHumansLithiumPotassium ChannelsProtein StabilityProtein SubunitsRecombinant Fusion ProteinsSodiumConceptsPotassium channel activityΑ-subunitPotassium channel α-subunitChannel activityGene codesFunctional reconstitutionMembrane vesiclesChannel familyCell typesPlanar bilayer membranesPotassium channelsBilayer membranesExpressionReconstitutionPurificationProteinVesiclesMembraneActivityFamilySodium ions
2010
A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events
Bian S, Koo BW, Kelleher S, Santos-Sacchi J, Navaratnam DS. A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events. American Journal Of Physiology - Cell Physiology 2010, 299: c828-c835. PMID: 20631244, PMCID: PMC3774197, DOI: 10.1152/ajpcell.00182.2010.Peer-Reviewed Original Research