2024
Tissue spaces are reservoirs of antigenic diversity for Trypanosoma brucei
Beaver A, Keneskhanova Z, Cosentino R, Weiss B, Awuoche E, Smallenberger G, Buenconsejo G, Crilly N, Smith J, Hakim J, Zhang B, Bobb B, Rijo-Ferreira F, Figueiredo L, Aksoy S, Siegel T, Mugnier M. Tissue spaces are reservoirs of antigenic diversity for Trypanosoma brucei. Nature 2024, 1-8. PMID: 39478231, DOI: 10.1038/s41586-024-08151-z.Peer-Reviewed Original ResearchVariant surface glycoproteinAntigenic diversityAntigenic variationHigh-throughput sequencing approachDense variant surface glycoproteinPopulations of T. bruceiDynamics of antigenic variationGenomic repertoirePathogen diversificationSequencing approachExtravascular spaceTrypanosoma bruceiVariation in vivoHost immune systemAntigenic variation in vivoSurface glycoproteinSlow immune responseDiversitySwitching expressionParasite populations
2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis