2008
A relaxed active site after exon ligation by the group I intron
Lipchock SV, Strobel SA. A relaxed active site after exon ligation by the group I intron. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 5699-5704. PMID: 18408159, PMCID: PMC2311373, DOI: 10.1073/pnas.0712016105.Peer-Reviewed Original ResearchConceptsActive siteMetal ionsRelaxed active siteActive site metal ionScissile phosphateDirect metal coordinationHydrogen bonding contactsMetal coordinationBonding contactsTransition stateCrystal structureThermodynamic measurementsIonsGround stateStructureCoordinationStructural observationsTertiary interactionsIntron releaseExon ligationGroup I intronPhosphateComplexesReactionSecond step
1999
Nucleotide Analog Interference Mapping of the Hairpin Ribozyme: Implications for Secondary and Tertiary Structure Formation
Ryder S, Strobel S. Nucleotide Analog Interference Mapping of the Hairpin Ribozyme: Implications for Secondary and Tertiary Structure Formation. Journal Of Molecular Biology 1999, 291: 295-311. PMID: 10438622, DOI: 10.1006/jmbi.1999.2959.Peer-Reviewed Original ResearchConceptsNucleotide analog interference mappingLoop AMinor groove edgeTertiary structure formationProper foldingThree-dimensional structureHairpin ribozymeNucleotide conservationNMR structureLoop BInterference mappingTertiary interactionsLoop B.Purine nucleotidesBiochemical evidenceRibozymeIntact ribozyme