1997
RNA seeing double: Close-packing of helices in RNA tertiary structure
Strobel S, Doudna J. RNA seeing double: Close-packing of helices in RNA tertiary structure. Trends In Biochemical Sciences 1997, 22: 262-266. PMID: 9255068, DOI: 10.1016/s0968-0004(97)01056-6.Peer-Reviewed Original ResearchConceptsHigher-order structure formationPacking of helicesChromosome maintenanceNoncanonical base pairsRNA processingDouble-helical segmentsRNA tertiary structureProtein biosynthesisRecognition motifRNA moleculesTertiary structureBase pairsHelical packingRNAEssential roleProteinActive siteBiosynthesisComplex architectureFoldingMotifHelixAssemblyDifferent strategies
1991
Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation
Strobel S, Doucette-Stamm L, Riba L, Housman D, Dervan P. Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation. Science 1991, 254: 1639-1642. PMID: 1836279, DOI: 10.1126/science.1836279.Peer-Reviewed Original ResearchConceptsHuman chromosome 4Human chromosomesChromosome 4Disease genesTriple helix formationEntire candidate regionSpecific DNA segmentsCandidate gene lociSite-specific cleavageGigabase pairsHuman genomeEnzymatic cleavageRecognition motifDNA segmentsGene locusGenomic DNAHuntington's disease geneHuman geneticsTarget siteChromosomesSized fragmentsCandidate regionsCleavageGenomePhysical isolationSingle-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation
Strobel S, Dervan P. Single-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation. Nature 1991, 350: 172-174. PMID: 1848684, DOI: 10.1038/350172a0.Peer-Reviewed Original ResearchConceptsTriple helix formationGenomic DNABase pairsYeast genomic DNAHelix formationSequence-specific DNALarge genomic DNAGenomic libraryMegabase pairsPhysical mappingEnzymatic cleavageRecognition motifSpecificity of bindingChromosomal DNARestriction endonuclease digestionManageable fragmentsRecognition sequenceGenetic markersDNA showRestriction enzymesQuantitative cleavageLarge DNADNAEndonuclease digestionChromosomes