2014
Functionally Important Carboxyls in a Bacterial Homologue of the Vesicular Monoamine Transporter (VMAT)*
Yaffe D, Vergara-Jaque A, Shuster Y, Listov D, Meena S, Singh SK, Forrest LR, Schuldiner S. Functionally Important Carboxyls in a Bacterial Homologue of the Vesicular Monoamine Transporter (VMAT)*. Journal Of Biological Chemistry 2014, 289: 34229-34240. PMID: 25336661, PMCID: PMC4256354, DOI: 10.1074/jbc.m114.607366.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArginineBacterial ProteinsBiogenic MonoaminesBrevibacillusCarrier ProteinsDrug Resistance, BacterialEscherichia coliEvolution, MolecularGene ExpressionHistidineModels, MolecularMolecular Sequence DataProtein FoldingRatsRecombinant ProteinsSequence AlignmentStructural Homology, ProteinStructure-Activity RelationshipSubstrate SpecificitySynaptic TransmissionVesicular Monoamine Transport ProteinsConceptsBacterial multidrug transportersMultidrug transporterBacterial homologueVesicular monoamine transporterRat vesicular monoamine transporterMonoamine transportersPreliminary biochemical characterizationMammalian transportersTransmembrane helicesMammalian counterpartsTransmembrane segmentsMammalian organismsEvolutionary aspectsMajor facilitatorBiochemical characterizationImportant carboxylB. brevisHomology modelBiochemical studiesTransportersHomologuesCarboxyl residuesAntibiotic resistanceOrganismsNeurotransportersStructure and Regulatory Interactions of the Cytoplasmic Terminal Domains of Serotonin Transporter
Fenollar-Ferrer C, Stockner T, Schwarz TC, Pal A, Gotovina J, Hofmaier T, Jayaraman K, Adhikary S, Kudlacek O, Mehdipour AR, Tavoulari S, Rudnick G, Singh SK, Konrat R, Sitte HH, Forrest LR. Structure and Regulatory Interactions of the Cytoplasmic Terminal Domains of Serotonin Transporter. Biochemistry 2014, 53: 5444-5460. PMID: 25093911, PMCID: PMC4147951, DOI: 10.1021/bi500637f.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCircular DichroismCytoplasmFluorescence Resonance Energy TransferHumansMagnetic Resonance SpectroscopyModels, MolecularMolecular Sequence DataProtein ConformationProtein FoldingProtein Structure, SecondaryProtein Structure, TertiarySerotonin Plasma Membrane Transport ProteinsConceptsTerminal domainStructures of homologuesYellow fluorescent protein tagProtein-protein interactionsFluorescent protein tagsFluorescence resonance energy transfer signalN-terminal domainCarboxy-terminal endHelix-breaking residuesCyan fluorescent proteinEnergy transfer signalHuman serotonin transporterNSS familyConformational cycleCircular dichroism spectroscopyProtein tagsCytoplasmic segmentRegulatory interactionsTransmembrane regionUptake of neurotransmittersInteraction partnersRegulatory mechanismsSerotonin transporterBiophysical approachesFluorescent protein
2009
Crystal structure and association behaviour of the GluR2 amino‐terminal domain
Jin R, Singh SK, Gu S, Furukawa H, Sobolevsky AI, Zhou J, Jin Y, Gouaux E. Crystal structure and association behaviour of the GluR2 amino‐terminal domain. The EMBO Journal 2009, 28: 1812-1823. PMID: 19461580, PMCID: PMC2699365, DOI: 10.1038/emboj.2009.140.Peer-Reviewed Original ResearchConceptsAmino-terminal domainLigand-gated ion channel proteinsReceptor assemblyModular domain architectureIon channel proteinsFast excitatory neurotransmissionSame subfamilyMolecular basisReceptor subfamiliesChannel proteinsMolecular processesSubfamiliesAMPA receptor GluR1Ionotropic glutamate receptorsCrystal structureExcitatory neurotransmissionAssemblyGlutamate receptorsPropose mechanismsReceptorsSubunitsDomainProteinAssemblagesNMDA receptors
2005
Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters
Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters. Nature 2005, 437: 215-223. PMID: 16041361, DOI: 10.1038/nature03978.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacteriaBacterial ProteinsBinding SitesBiological TransportChloridesCrystallography, X-RayHydrophobic and Hydrophilic InteractionsLeucineMembrane Transport ProteinsModels, MolecularMolecular Sequence DataNeurotransmitter AgentsSequence AlignmentSodiumStructure-Activity RelationshipWaterConceptsBacterial homologueProtein coreDependent neurotransmitter transportersTransmembrane helicesTransmembrane segmentsAquifex aeolicusUptake of neurotransmittersSubstrate bindingNeurotransmitter transportersMain-chain atomsMembrane bilayerDependent transportersElectrochemical gradientIon binding siteTransportersHelix dipole