2017
Conformational dynamics of a neurotransmitter:sodium symporter in a lipid bilayer
Adhikary S, Deredge DJ, Nagarajan A, Forrest LR, Wintrode PL, Singh SK. Conformational dynamics of a neurotransmitter:sodium symporter in a lipid bilayer. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e1786-e1795. PMID: 28223522, PMCID: PMC5347597, DOI: 10.1073/pnas.1613293114.Peer-Reviewed Original ResearchConceptsSodium symportersMembrane proteinsMammalian membrane proteinsConformational mechanismIntegral membrane proteinsPhospholipid bilayer nanodiscsSolution spectroscopyDetergent-solubilized stateExtracellular loop 2Site-specific labelingSmall molecule neurotransmittersBilayer nanodiscsHydrogen-deuterium exchangeX-ray crystallographyCysteine accessibilityConformational dynamicsMembrane mimicsMolecular dynamics simulationsLoop 2Endogenous cysteineHelices 1ALeuTLipid bilayersProteinSymporter
2008
Structural models of inhibition in neurotransmitter sodium symporters
Singh S, Gouaux J. Structural models of inhibition in neurotransmitter sodium symporters. The FASEB Journal 2008, 22: 254.2-254.2. DOI: 10.1096/fasebj.22.1_supplement.254.2.Peer-Reviewed Original ResearchNeurotransmitter sodium symportersSodium symportersNovel noncompetitive inhibitorsX-ray crystallographyAtomic-level insightsSteady-state kinetic dataLeucine transporterSteady-state kineticsMolecular principlesRational designSmall moleculesIon gradientsKinetic dataDissociation assaysAddictive compoundInhibitor actionCritical roleNoncompetitive modeStructural paradigmSymporterNoncompetitive inhibitorAllosteric antagonistsGlial cytoplasmSynaptic transmissionLeuT