1998
An amino acid as a cofactor for a catalytic polynucleotide
Roth A, Breaker R. An amino acid as a cofactor for a catalytic polynucleotide. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 6027-6031. PMID: 9600911, PMCID: PMC27579, DOI: 10.1073/pnas.95.11.6027.Peer-Reviewed Original ResearchConceptsAmino acidsRate enhancementDramatic rate enhancementMetal ion cofactorsAbsence of enzymeGeneral base catalystSuperior polymerChemical catalysisBiological catalystsRNA cleavage reactionCatalytic potentialOrganic cofactorImidazole groupsNatural ribozymesRNA worldBase catalystChemical groupsCatalytic mechanismCatalytic DNACleavage reactionIon cofactorProtein enzymesStructural foldingSubstrate cleavageConstituent amino acids
1997
DNA enzymes
Breaker R. DNA enzymes. Nature Biotechnology 1997, 15: 427-431. PMID: 9131619, DOI: 10.1038/nbt0597-427.Peer-Reviewed Original Research
1996
In vitro selection of self-cleaving DNAs
Carmi N, Shultz L, Breaker R. In vitro selection of self-cleaving DNAs. Cell Chemical Biology 1996, 3: 1039-1046. PMID: 9000012, DOI: 10.1016/s1074-5521(96)90170-2.Peer-Reviewed Original ResearchConceptsIndividual catalystsCatalytic DNAEnzyme-like activityChemical transformationsSole cofactorRate enhancementAdditional reactionsCu2DNA enzymeCatalystDNA cleavageBiological contextVitro SelectionUncatalyzed rateOxidative mechanismsDNAFurther optimizationDistinct classesRibozymeHydroxylDeoxyribozymesBiocatalystReactionCleavageCofactor