1999
Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group
Li Y, Breaker R. Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group. Journal Of The American Chemical Society 1999, 121: 5364-5372. DOI: 10.1021/ja990592p.Peer-Reviewed Original ResearchSpecific base catalysisBase catalysisHydroxyl groupsRNA phosphodiesterRate enhancementMaximum rate enhancementGeneral base catalysisEnzyme mimicsAlkaline pH conditionsHydroxide ionTransfer reactionsReaction conditionsCatalysisBase-catalyzed cleavageDivalent magnesiumRNA polymersDNA/RNARNA linkageTransesterification reactionPH conditionsCleavage mechanismStrand scissionRNA transesterificationBiological systemsDominant pathwayPhosphorylating DNA with DNA
Li Y, Breaker R. Phosphorylating DNA with DNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 2746-2751. PMID: 10077582, PMCID: PMC15840, DOI: 10.1073/pnas.96.6.2746.Peer-Reviewed Original ResearchConceptsSubstrate recognition patternRandom sequence poolsKinase-like activityStandard NTPsATP hydrolysisSpecific target DNAVitro SelectionIndividual DNAProtein enzymesDNADNTP substratesMultiple turnoversDeoxyribozymesEnzymeTarget DNABiological systemsDeoxyribose moietyGTPNTPsRNAATPDNTPsCatalytic potentialSelectionRibose
1994
A DNA enzyme that cleaves RNA
Breaker R, Joyce G. A DNA enzyme that cleaves RNA. Cell Chemical Biology 1994, 1: 223-229. PMID: 9383394, DOI: 10.1016/1074-5521(94)90014-0.Peer-Reviewed Original ResearchConceptsDNA enzymeRNA enzymeDependent cleavageSimilarity of DNATypes of RNACatalytic domainDNA sequencesRandom nucleotidesRapid turnoverRNASuccessive roundsEnzymeDNACatalytic rateSelective amplificationBiological systemsTurnover rateSequenceCleavageIntermolecular contextNucleotidesPopulationTurnoverPhosphoesters