2022
Short Bowel Syndrome and Intestinal Lengthening Procedures
Salvi P, Cowles R. Short Bowel Syndrome and Intestinal Lengthening Procedures. 2022, 345-356. DOI: 10.1007/978-3-030-96542-6_31.ChaptersIntestinal lengthening proceduresParenteral nutritionLengthening proceduresIntestinal transplantSurgical interventionMultidisciplinary intestinal rehabilitation programParenteral nutrition failureIntestinal rehabilitation programShort bowel syndromeSmall bowel resectionSignificant physiologic consequencesCompensatory-adaptive changesBowel functionBowel resectionBowel syndromeIntestinal transitIntestinal adaptationIntestinal lossSpecialized epithelial cellsMucosal growthRehabilitation programPhysiologic consequencesDistal ileumPatientsFat absorptionJejunoileal mucosal growth in mice with a limited microbiome
Shaughnessy MP, Park CJ, Salvi PS, Cowles RA. Jejunoileal mucosal growth in mice with a limited microbiome. PLOS ONE 2022, 17: e0266251. PMID: 35349599, PMCID: PMC8963542, DOI: 10.1371/journal.pone.0266251.Peer-Reviewed Original ResearchConceptsIntestinal mucosal growthMucosal surface areaSmall intestinal mucosal growthCrypt proliferation indexCONV miceGF miceMucosal growthVillus heightEnteral antibioticsCrypt depthCell proportionSmall intestinal villus heightGerm-free miceBroad-spectrum antibioticsConventional floraIntestinal villus heightGerm-free conditionsEnteral administrationMucosal homeostasisGnotobiotic miceProliferation indexEnterocyte proliferationMiceTaller villiGF conditions
2020
The M1 muscarinic acetylcholine receptor in the crypt stem cell compartment mediates intestinal mucosal growth
Greig CJ, Armenia SJ, Cowles RA. The M1 muscarinic acetylcholine receptor in the crypt stem cell compartment mediates intestinal mucosal growth. Experimental Biology And Medicine 2020, 245: 1194-1199. PMID: 32611198, PMCID: PMC7437377, DOI: 10.1177/1535370220938375.Peer-Reviewed Original ResearchConceptsMuscarinic acetylcholine receptorsM1 muscarinic acetylcholine receptorAcetylcholine receptorsStem cell compartmentPotential future therapeutic targetsCell compartmentIntestinal mucosal homeostasisIntestinal mucosal growthFuture therapeutic targetsMucosal homeostasisMucosal growthTherapeutic targetSpecific subtypesProliferative parametersReceptorsFuture studiesDemonstrate increasesCritical roleNovel informationCompartmentsSubtypes
2019
Potentiation of serotonin signaling leads to increased carbohydrate and lipid absorption in the murine small intestine
Park CJ, Armenia SJ, Shaughnessy MP, Greig CJ, Cowles RA. Potentiation of serotonin signaling leads to increased carbohydrate and lipid absorption in the murine small intestine. Journal Of Pediatric Surgery 2019, 54: 1245-1249. PMID: 30879746, DOI: 10.1016/j.jpedsurg.2019.02.027.Peer-Reviewed Original ResearchConceptsPotentiation of serotoninSmall intestineMucosal growthLipid absorptionSmall intestinal mucosal growthMucosal growth factorsSerotonin reuptake transporterSERT knockout miceDistal small intestineIntestinal mucosal growthCrypt cell proliferationT-testMucosal surface areaMurine small intestineStudent's t-testEnteric serotoninIntestinal failureIntestinal adaptationReuptake transporterDeficient miceWT animalsIntestinal homeostasisFatty acid analogueCrypt depthVillus heightOrally Dosed Citalopram Stimulates Small Intestinal Mucosal Growth
Zhang L, Greig CJ, Cowles RA. Orally Dosed Citalopram Stimulates Small Intestinal Mucosal Growth. Journal Of Surgical Research 2019, 236: 326-331. PMID: 30694773, DOI: 10.1016/j.jss.2018.11.033.Peer-Reviewed Original ResearchConceptsMucosal surface areaSelective serotonin reuptake inhibitorsSerotonin reuptake inhibitorsIntestinal mucosal growthVillus heightVillus widthD citalopramReuptake inhibitorsMucosal growthCrypt depthCrypt widthSmall intestinal mucosal growthShort bowel syndromeT-testSerious intestinal disordersStudent's t-testOral agentsBowel syndromeOral therapyEnteral administrationC57BL/6 miceIleal segmentIntestinal disordersDay 21Day 7
2018
The 5-HT4 Receptor Agonist Prucalopride Stimulates Mucosal Growth and Enhances Carbohydrate Absorption in the Ileum of the Mouse
Park CJ, Armenia SJ, Zhang L, Cowles RA. The 5-HT4 Receptor Agonist Prucalopride Stimulates Mucosal Growth and Enhances Carbohydrate Absorption in the Ileum of the Mouse. Journal Of Gastrointestinal Surgery 2018, 23: 1198-1205. PMID: 30109470, DOI: 10.1007/s11605-018-3907-6.Peer-Reviewed Original ResearchConceptsDistal small bowelD-xylose absorptionCrypt cell proliferationSmall intestineMurine small intestineSmall bowelMucosal growthSpecific agonistCrypt depthGlucose absorptionIntestinal mucosal surface areaAdult wild-type miceMucosal growth factorsPotentiation of serotoninReceptor agonist prucaloprideDistal small intestineCell proliferationIntestinal mucosal growthWild-type miceReceptor-specific agonistsMiddle small intestineMucosal surface areaEnteric serotoninOral gavageCarbohydrate absorption
2017
Improved capacity to evaluate changes in intestinal mucosal surface area using mathematical modeling
Greig CJ, Cowles RA. Improved capacity to evaluate changes in intestinal mucosal surface area using mathematical modeling. Microscopy Research And Technique 2017, 80: 793-798. PMID: 28295852, DOI: 10.1002/jemt.22866.Peer-Reviewed Original ResearchConceptsMucosal surface areaIntestinal mucosal surface areaVillus heightCrypt widthMucosal growthSelective serotonin reuptakeIntestinal mucosal growthEffects of interventionsFunctional intestinal mucosaEnhanced serotoninBowel diameterSerotonin reuptakeIntestinal mucosaMouse modelIleal sectionsTransporter moleculesMorphometric measuresComprehensive assessmentSSRIsMorphometric parametersMucosaSerotoninStandard measuresTherapyReuptake
2016
Enhanced serotonin signaling increases intestinal neuroplasticity
Greig CJ, Gandotra N, Tackett JJ, Bamdad MC, Cowles RA. Enhanced serotonin signaling increases intestinal neuroplasticity. Journal Of Surgical Research 2016, 206: 151-158. PMID: 27916355, DOI: 10.1016/j.jss.2016.07.021.Peer-Reviewed Original ResearchConceptsSelective serotonin reuptake inhibitorsIntestinal mucosal growthSERT knockoutMucosal growthEnterocyte proliferationEnteric nervous systemSerotonin reuptake inhibitorsMassive bowel resectionSerotonin reuptake transporterAge-matched WTGAP-43 expressionWild-type miceSynthesis inhibitionMarkers of neuroplasticityImportant regulatorReversal of effectsEpithelial cell compartmentWestern blot analysisBowel resectionReuptake inhibitorsWT miceReuptake transporterMucosal homeostasisIntestinal segmentsCrypt depth
2012
Neuronal Serotonin Regulates Growth of the Intestinal Mucosa in Mice
Gross ER, Gershon MD, Margolis KG, Gertsberg ZV, Li Z, Cowles RA. Neuronal Serotonin Regulates Growth of the Intestinal Mucosa in Mice. Gastroenterology 2012, 143: 408-417.e2. PMID: 22609381, PMCID: PMC3687781, DOI: 10.1053/j.gastro.2012.05.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCholine O-AcetyltransferaseCholinergic NeuronsEnterochromaffin CellsIntestinal MucosaKetanserinMaleMiceMice, Inbred C57BLMice, KnockoutMuscarinic AntagonistsReceptor, Serotonin, 5-HT2AScopolamineSelective Serotonin Reuptake InhibitorsSerotonergic NeuronsSerotoninSerotonin AntagonistsSerotonin Plasma Membrane Transport ProteinsTryptophan HydroxylaseConceptsSelective serotonin re-uptake inhibitorsSerotonin re-uptake inhibitorsRe-uptake inhibitorsWild-type miceIntestinal mucosaSerotonin re-uptake transporterCholinergic submucosal neuronsIntestinal mucosal epitheliumRe-uptake transporterTryptophan hydroxylase 1Tryptophan hydroxylase 2Submucosal neuronsCholinergic neuronsCholine acetyltransferaseNeuronal serotoninMucosal growthMucosal epitheliumMucosal cellsSERT knockoutEpithelial proliferationAbstractTextMiceHydroxylase 2MucosaNeural precursors