2024
JAK/STAT signaling pathway affects CCR5 expression in human CD4+ T cells
Wang L, Yukselten Y, Nuwagaba J, Sutton R. JAK/STAT signaling pathway affects CCR5 expression in human CD4+ T cells. Science Advances 2024, 10: eadl0368. PMID: 38507500, PMCID: PMC10954213, DOI: 10.1126/sciadv.adl0368.Peer-Reviewed Original ResearchConceptsCD4<sup>+</sup> T cellsT cellsCCR5 expressionPrimary CD4<sup>+</sup> T cellsCD4+ T cellsHuman CD4+ T cellsHuman primary CD4<sup>+</sup> T cellsGlucocorticoid-regulated kinase 1HIV cure agendaHIV co-receptorsSignaling pathwayCCR5 levelsJAK/STAT inhibitor tofacitinibViremic patientsHIV patientsHIV infectionInhibitor tofacitinibJAK/STAT signaling pathwayCCR5Co-receptorHIVProtein levelsCCR2/CCR5Adverse effectsDown-regulation
2023
Correlation of a commercial platform’s results with post-vaccination SARS-CoV-2 neutralizing antibody response and clinical host factors
Slotkin R, Kyriakides T, Kundu A, Stack G, Sutton R, Gupta S. Correlation of a commercial platform’s results with post-vaccination SARS-CoV-2 neutralizing antibody response and clinical host factors. PLOS ONE 2023, 18: e0289713. PMID: 37643190, PMCID: PMC10464955, DOI: 10.1371/journal.pone.0289713.Peer-Reviewed Original Research
2018
Identification and characterization of HIV positive Ethiopian elite controllers in both Africa and Israel
Kiros Y, Elinav H, Gebreyesus A, Gebremeskel H, Azar J, Chemtob D, Abreha H, Elbirt D, Shahar E, Chowers M, Turner D, Grossman Z, Haile A, Sutton R, Maayan S, Wolday D. Identification and characterization of HIV positive Ethiopian elite controllers in both Africa and Israel. HIV Medicine 2018, 20: 33-37. PMID: 30318718, PMCID: PMC6510948, DOI: 10.1111/hiv.12680.Peer-Reviewed Original ResearchConceptsStable CD4 countsElite controllersCD4 countAntiretroviral treatmentIsraeli cohortAfrican cohortART-naïve subjectsHIV elite controllersLarge African cohortHIV-positive patientsUndetectable viral loadCells/μLWarrants further investigationEthiopian patientsViral loadOverall prevalenceCohortPatientsPrevalenceFirst cohortUnique subgroupPossible environmental factorsSignificant differencesHomogenous populationSelection bias
2001
Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar M, Keller B, Makalou N, Sutton R. Systematic Determination of the Packaging Limit of Lentiviral Vectors. Human Gene Therapy 2001, 12: 1893-1905. PMID: 11589831, DOI: 10.1089/104303401753153947.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsBlotting, SouthernCapsidCell LineDNAEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesGenetic VectorsHeLa CellsHIV-1HumansLentivirusMembrane GlycoproteinsModels, GeneticPlasmidsReverse Transcriptase Polymerase Chain ReactionRNA, ViralTransduction, GeneticTransfectionViral Envelope ProteinsConceptsPackaging limitLentiviral vectorsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1Oncoretroviral vectorsTherapeutic deliveryVesicular stomatitis virus G proteinMeasurable titersLentiviral particlesLow titersViral titersType 1Great potentialForeign DNATitersProviral RNAVirus G proteinViral encapsidationHIVG proteinsLarge vectorsChromosomal DNA fragmentLimited utilityCells
2000
Transduction of Human PBMC-Derived Dendritic Cells and Macrophages by an HIV-1-Based Lentiviral Vector System
Schroers R, Sinha I, Segall H, Schmidt-Wolf I, Rooney C, Brenner M, Sutton R, Chen S. Transduction of Human PBMC-Derived Dendritic Cells and Macrophages by an HIV-1-Based Lentiviral Vector System. Molecular Therapy 2000, 1: 171-179. PMID: 10933928, DOI: 10.1006/mthe.2000.0027.Peer-Reviewed Original Research
1999
Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent
Sutton R, Reitsma M, Uchida N, Brown P. Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent. Journal Of Virology 1999, 73: 3649-3660. PMID: 10196257, PMCID: PMC104140, DOI: 10.1128/jvi.73.5.3649-3660.1999.Peer-Reviewed Original ResearchConceptsProgenitor hematopoietic stem cellsHematopoietic stem cellsCell cycleG0 cellsStem cellsTransduction ratesG2/M phaseReplication intermediatesCellular factorsHuman immunodeficiency virus type 1 vectorsTransduction efficiencyTransductionAddition of cytokinesM phaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1Presence of cytokinesLentivirus vectorFurther characterizationReverse transcriptionDistinct subpopulationsG2/M fractionVirus type 1CellsTime of initiation
1998
HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells
Uchida N, Sutton R, Friera A, He D, Reitsma M, Chang W, Veres G, Scollay R, Weissman I. HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 11939-11944. PMID: 9751769, PMCID: PMC21744, DOI: 10.1073/pnas.95.20.11939.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD34Base SequenceColony-Forming Units AssayDNA PrimersG1 PhaseGene ExpressionGene Transfer TechniquesGenes, ReporterGenetic TherapyGenetic VectorsGreen Fluorescent ProteinsHematopoietic Stem CellsHIV-1HumansIn Vitro TechniquesLeukemia Virus, MurineLuminescent ProteinsPhenotypeResting Phase, Cell CycleThy-1 AntigensTransduction, GeneticConceptsTransgene expressionHigh-efficiency gene transferSingle-step transductionGene delivery systemsGreen fluorescent proteinHematopoietic stem cellsHuman HSC subsetsVector supernatantsEfficient transductionTransduction efficiencyStem cellsHuman hematopoietic stem cellsMurine leukemia virusHIV vectorsDelivery systemGene transferGFP expressionHuman cell typesStem cell phenotypeReplication-defective HIVBone marrow stromal cellsLeukemia virusFluorescent proteinMarrow stromal cellsG0/G1Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells
Sutton R, Wu H, Rigg R, Böhnlein E, Brown P. Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells. Journal Of Virology 1998, 72: 5781-5788. PMID: 9621037, PMCID: PMC110379, DOI: 10.1128/jvi.72.7.5781-5788.1998.Peer-Reviewed Original ResearchConceptsHuman hematopoietic stem cellsHematopoietic stem cellsStem cellsMarker gene expressionLentivirus vector systemGene transfer agentsMarker gene productLong-term persistenceGene productsGene expressionHuman immunodeficiency virus type 1 vectorsIntegrated provirusStable expressionVector systemTransduction ratesCellsExpressionHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1MitosisTransductionDeletionOncoretrovirusesVpr
1996
Identification of a major co-receptor for primary isolates of HIV-1
Deng H, Liu R, Ellmeier W, Choe S, Unutmaz D, Burkhart M, Marzio P, Marmon S, Sutton R, Hill C, Davis C, Peiper S, Schall T, Littman D, Landau N. Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996, 381: 661-666. PMID: 8649511, DOI: 10.1038/381661a0.Peer-Reviewed Original Research