2017
From in silico hit to long-acting late-stage preclinical candidate to combat HIV-1 infection
Kudalkar SN, Beloor J, Quijano E, Spasov KA, Lee WG, Cisneros JA, Saltzman WM, Kumar P, Jorgensen WL, Anderson KS. From in silico hit to long-acting late-stage preclinical candidate to combat HIV-1 infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 115: e802-e811. PMID: 29279368, PMCID: PMC5789948, DOI: 10.1073/pnas.1717932115.Peer-Reviewed Original ResearchConceptsHIV-1 drugsDrug-resistant HIV-1 strainsHIV-1 drug-resistant strainsPreclinical candidateDrug-resistant HIV-1HIV-1-infected T cellsDaily treatment regimensActive antiretroviral therapyT cell lossSynergistic antiviral activityHIV-1 infectionAnti-HIV-1 agentsCombination drug regimensHIV-1 strainsMajor therapeutic challengeHIV-1 pandemicPlasma drug concentrationsDrug-resistant strainsVivo pharmacokinetic behaviorAntiretroviral therapyDrug regimensTherapeutic challengeViral loadTreatment regimensSingle dose
2007
Transvascular delivery of small interfering RNA to the central nervous system
Kumar P, Wu H, McBride JL, Jung KE, Hee Kim M, Davidson BL, Kyung Lee S, Shankar P, Manjunath N. Transvascular delivery of small interfering RNA to the central nervous system. Nature 2007, 448: 39-43. PMID: 17572664, DOI: 10.1038/nature05901.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlood-Brain BarrierBrainCell LineDrug Delivery SystemsEncephalitis Virus, JapaneseEncephalitis, JapaneseGene SilencingGenetic VectorsGlycoproteinsGreen Fluorescent ProteinsHeLa CellsHumansLentivirusLiposomesMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred NODMice, SCIDMolecular Sequence DataNeuronsOligopeptidesRabies virusReceptors, NicotinicRecombinant ProteinsRNA, Small InterferingSuperoxide DismutaseSuperoxide Dismutase-1Viral ProteinsConceptsRVG-9RBlood-brain barrierRabies virus glycoproteinNeuronal cellsTransvascular deliveryFatal viral encephalitisCentral nervous systemTherapeutic moleculesIntravenous treatmentViral encephalitisInflammatory cytokinesAntiviral siRNAIntravenous injectionNervous systemAcetylcholine receptorsNeurological diseasesAnti-peptide antibodiesChimaeric peptidesNoninvasive approachDelivery of siRNABrainSiRNAMiceVirus glycoproteinRobust protection