2008
Mutations Outside the Anisomycin-Binding Site Can Make Ribosomes Drug-Resistant
Blaha G, Gürel G, Schroeder SJ, Moore PB, Steitz TA. Mutations Outside the Anisomycin-Binding Site Can Make Ribosomes Drug-Resistant. Journal Of Molecular Biology 2008, 379: 505-519. PMID: 18455733, PMCID: PMC2442718, DOI: 10.1016/j.jmb.2008.03.075.Peer-Reviewed Original Research
2005
Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted
Tu D, Blaha G, Moore PB, Steitz TA. Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted. Extremophiles 2005, 9: 427-435. PMID: 15970993, DOI: 10.1007/s00792-005-0459-y.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, SouthernChromosome MappingCrystallography, X-RayDNADNA PrimersElectronsEscherichia coli ProteinsGene DeletionGenetic TechniquesHaloarcula marismortuiModels, ChemicalModels, GeneticModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMutationOperonPlasmidsReverse Transcriptase Polymerase Chain ReactionRibosomal ProteinsRNA-Binding ProteinsRNA, RibosomalRNA, Ribosomal, 23SRRNA OperonSucroseConceptsRRNA operonsHaloarcula marismortuiChromosomal rRNA operonsLarge ribosomal subunitRibosomal protein L22Wild-type organismsSite-directed mutagenesisAmino acid deletionBacteriorhodopsin geneRrnB operonProtein L22Ribosomal subunitRRNA geneGene replacementOperonWild typeRich mediumAcid deletionSuch mutationsGenesHalobacterium halobiumStructural consequencesMarismortuiAtomic resolutionStrainsStructures of MLSBK Antibiotics Bound to Mutated Large Ribosomal Subunits Provide a Structural Explanation for Resistance
Tu D, Blaha G, Moore PB, Steitz TA. Structures of MLSBK Antibiotics Bound to Mutated Large Ribosomal Subunits Provide a Structural Explanation for Resistance. Cell 2005, 121: 257-270. PMID: 15851032, DOI: 10.1016/j.cell.2005.02.005.Peer-Reviewed Original ResearchConceptsLarge ribosomal subunitRibosomal subunitWild-type subunitsWild-type affinityResidue deletion mutantEubacterial ribosomesLarge subunitDeletion mutantsSubunitsResistance phenotypeStreptogramin AVirginiamycin SStructural explanationMutantsRibosomesA2058GAffinityL22BindsCrystal structurePhenotypeBinding
2002
The Structures of Four Macrolide Antibiotics Bound to the Large Ribosomal Subunit
Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA. The Structures of Four Macrolide Antibiotics Bound to the Large Ribosomal Subunit. Molecular Cell 2002, 10: 117-128. PMID: 12150912, DOI: 10.1016/s1097-2765(02)00570-1.Peer-Reviewed Original Research