The mechanism of rate-limiting motions in enzyme function
Watt ED, Shimada H, Kovrigin EL, Loria JP. The mechanism of rate-limiting motions in enzyme function. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 11981-11986. PMID: 17615241, PMCID: PMC1924554, DOI: 10.1073/pnas.0702551104.Peer-Reviewed Original ResearchConceptsEnzyme functionProtein motionsCluster of residuesRate-limiting enzymatic stepEnzyme active siteActive siteNMR relaxation experimentsSingle residueEnzymatic stepsConformational flexibilityRate-limiting stepKey modulatorHistidine-48RNaseResiduesSingle siteMutantsSitesMutationsEnzymeH48Solvent isotope effectFunctionHallmarkExchangeable protons