2015
Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival
Kayisli UA, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson HC, Shapiro J, Summerfield T, Huang SJ, Prelle K, Schatz F, Lockwood CJ. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 5153-5158. PMID: 25847994, PMCID: PMC4413279, DOI: 10.1073/pnas.1424814112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CountCell DifferentiationCell MovementCell ProliferationCell SurvivalCells, CulturedChemokine CCL2Contraceptive Agents, FemaleDesogestrelEndometriumFemaleGene Expression ProfilingGene Expression RegulationGuinea PigsHumansMedroxyprogesterone AcetateModels, BiologicalMuscle, Smooth, VascularMyocytes, Smooth MuscleOvariectomyProgestinsProliferating Cell Nuclear AntigenSignal TransductionSTAT1 Transcription FactorConceptsVascular smooth muscle cellsChemokine ligand 2Medroxyprogesterone acetateEndometrial vasculatureVSMC proliferationCultured vascular smooth muscle cellsSmooth muscle cellsSmooth muscle cell survivalVascular smooth muscle cell survivalCell nuclear antigenFragile microvesselsΑSMA immunoreactivityEndometrial vesselsΑSMA stainingPericyte proliferationSTAT1 inhibitorGuinea pigsMuscle cellsMuscle cell survivalCCL2 effectsEtonogestrelMigration assaysNuclear antigenVSMC differentiationWomen
2014
Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells
Shapiro JP, Basar M, Kayisli UA, Guzeloglu-Kayisli O, Huang S, Suarez AA, Ozer HG, Schatz F, Lockwood CJ. Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells. Contraception 2014, 91: 253-260. PMID: 25529278, PMCID: PMC4395468, DOI: 10.1016/j.contraception.2014.11.005.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsUterine bleedingStanniocalcin-1Cultured human endometrial stromal cellsStromal cellsMicroarray analysisEndometrial biopsyTubal metaplasiaMRNA microarray analysisSide effectsLAPCComplex biological responseEtonogestrelHypoxia treatmentProgestinsHypoxiaPrimary culturesPotential mediatorsPotential targetProtein mediatorsBleedingExpression levelsMedium supernatantLong-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis
Guzeloglu-Kayisli O, Basar M, Shapiro J, Semerci N, Huang J, Schatz F, Lockwood C, Kayisli U. Long-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis. The Journal Of Clinical Endocrinology & Metabolism 2014, 99: e1957-e1966. PMID: 25029423, PMCID: PMC4184079, DOI: 10.1210/jc.2014-1770.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisC-Reactive ProteinCaspase 3Cell ProliferationCells, CulturedContraceptive Agents, FemaleCulture Media, ConditionedCytochromes cEndometriumEndothelial CellsEstradiolFemaleHumansMedroxyprogesterone AcetateMicrovesselsNerve Tissue ProteinsParacrine CommunicationProgestinsReactive Oxygen SpeciesStromal CellsConceptsHuman endometrial endothelial cellsNeuronal pentraxin 1Abnormal uterine bleedingUterine bleedingMedroxyprogesterone acetateReactive oxygen species generationCultured human endometrial endothelial cellsOxygen species generationHuman endometrial stromal cellsUterine blood flowEndometrium of womenEndometrial endothelial cellsEndometrial stromal cellsProgesterone receptor proteinCaspase-3Elevated reactive oxygen species (ROS) generationHESC-CMsNovel paracrine mechanismEndothelial cell apoptosisSpecies generationFragile microvesselsCytosolic cytochrome c levelsParacrine mechanismsBlood flowLAPCHypertension alters phosphorylation of VASP in brain endothelial cells
Arlier Z, Basar M, Kocamaz E, Kiraz K, Tanriover G, Kocer G, Arlier S, Giray S, Nasırcılar S, Gunduz F, Senturk U, Demir N. Hypertension alters phosphorylation of VASP in brain endothelial cells. International Journal Of Neuroscience 2014, 125: 288-297. PMID: 24894047, DOI: 10.3109/00207454.2014.930740.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood PressureBrainCase-Control StudiesCell Adhesion MoleculesCells, CulturedDisease Models, AnimalEndothelial CellsExercise TherapyGene Expression RegulationHumansHypertensionHypoxiaMicrofilament ProteinsOxygenPhosphoproteinsPhosphorylationRatsRats, Inbred SHRRats, Inbred WKYSerineStatistics, NonparametricSwimmingTime FactorsConceptsBrain endothelial cellsVASP expressionEndothelial cellsVasodilator-stimulated phosphoproteinVASP phosphorylationWestern blottingHypertension-induced alterationsIndirect salutary effectsEffects of hypertensionEffects of exerciseNormoxic endothelial cellsEndothelial barrier functionWKY ratsEndothelial changesTissue hypoxiaCerebral vasculatureHypertensionSalutary effectsExercise conditionsBarrier functionVascular systemExerciseAlters phosphorylationImmunocytochemistryBlotting
2012
Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis
Cakmak H, Basar M, Seval-Celik Y, Osteen KG, Duleba AJ, Taylor HS, Lockwood CJ, Arici A. Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis. Reproductive Sciences 2012, 19: 572-579. PMID: 22267540, PMCID: PMC3439122, DOI: 10.1177/1933719111430998.Peer-Reviewed Original ResearchConceptsCultured endometriotic cellsMonocyte chemotactic protein-1 expressionChemotactic protein-1 expressionMCP-1 productionEndometriotic cellsProtein-1 expressionEndometriotic implantsMouse modelMCP-1 messenger RNA expressionExerts anti-inflammatory effectsAnti-inflammatory effectsEffect of statinsAnti-inflammatory propertiesMCP-1 expressionMevalonate pathwayDose-dependent inhibitionNude mouse modelMessenger RNA expressionDose-dependent mannerEctopic endometriumEndometrial cellsPeritoneal surfaceStatinsPotential treatmentInhibitory effect
2011
Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1
Guzel E, Basar M, Ocak N, Arici A, Kayisli UA. Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1. Biology Of Reproduction 2011, 85: 121-127. PMID: 21389343, DOI: 10.1095/biolreprod.110.089532.Peer-Reviewed Original ResearchConceptsHSPA5 expressionHuman endometriumMenstrual cycleStromal cellsUnfolded protein responseNormal human endometriumEndometrial glandularEstradiol levelsHuman Endometrium1Possible inverse correlationIshikawa cellsEstrogen signalingLocal paracrineEndometriumSteroid hormonesAutocrine factorHeat shock 70EstrogenInverse correlationBidirectional regulationExpression levelsBidirectional interactionsShock 70Dynamic tissueHSPA5
2010
The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia
Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ. The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia. American Journal Of Pathology 2010, 177: 2472-2482. PMID: 20829438, PMCID: PMC2966804, DOI: 10.2353/ajpath.2010.091247.Peer-Reviewed Original ResearchConceptsGranulocyte-macrophage colony-stimulating factorColony-stimulating factorPathogenesis of preeclampsiaDendritic cellsDecidual cellsFirst trimester human decidual cellsGranulocyte-macrophage colony-stimulating factor (GM-CSF) expressionGenesis of preeclampsiaSystemic inflammatory stateInterleukin-1 βTumor necrosis factorHuman decidual cellsColony-stimulating factor expressionFactor expression levelsColony-stimulating factor productionGM-CSF expressionGranulocyte-macrophage colony-stimulating factor productionPreeclamptic deciduaShallow placentationInflammatory stateHuman deciduaNecrosis factorMurine modelPreeclampsiaMouse model
2008
Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells
Lockwood CJ, Yen CF, Basar M, Kayisli UA, Martel M, Buhimschi I, Buhimschi C, Huang SJ, Krikun G, Schatz F. Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells. American Journal Of Pathology 2008, 172: 1571-1579. PMID: 18467705, PMCID: PMC2408417, DOI: 10.2353/ajpath.2008.070629.Peer-Reviewed Original ResearchConceptsIL-6 levelsIL-6Decidual cellsGestational age-matched control placentasElevated IL-6 levelsIL-6 mRNA levelsCommon pregnancy disorderGenesis of preeclampsiaUteroplacental blood flowFetal-placental unitInterleukin-6 levelsPathogenesis of preeclampsiaPro-inflammatory cytokinesEndothelial cell dysfunctionInterleukin-6 expressionHuman decidual cellsReal-time quantitative RT-PCRTime quantitative RT-PCRQuantitative RT-PCRFetal morbidityDecidual macrophagesPreeclamptic womenSystemic inflammationPregnancy disordersControl placentas
2006
Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells
Selam B, Kayisli UA, Akbas GE, Basar M, Arici A. Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells. Biology Of Reproduction 2006, 75: 203-209. PMID: 16687653, DOI: 10.1095/biolreprod.105.045716.Peer-Reviewed Original ResearchConceptsFASLG expressionEndometrial stromal cellsStromal cellsChemokine ligand 2Protein expressionSemiquantitative RT-PCR analysisRT-PCR analysisFAS ligand expressionHuman endometriumCellular growthWestern blot analysisRate of apoptosisCultured endometrial stromal cellsExtracellular matrixJurkat cellsIncreased apoptotic rateHuman endometrial stromal cellsApoptotic moleculesLevels of CCL2Blot analysisDynamic tissueNumerous hormonesApoptosisHuman endometrial cellsSame cells