2008
Heterozygous expression of myocilin glaucoma mutants increases secretion of the mutant forms and reduces extracellular processed myocilin.
Aroca-Aguilar JD, Sánchez-Sánchez F, Martínez-Redondo F, Coca-Prados M, Escribano J. Heterozygous expression of myocilin glaucoma mutants increases secretion of the mutant forms and reduces extracellular processed myocilin. Molecular Vision 2008, 14: 2097-108. PMID: 19023451, PMCID: PMC2585175.Peer-Reviewed Original ResearchConceptsWild-type myocilinWild-type proteinMyocilin mutantsMutant myocilinMutant formsProteolytic processingMissense mutant formsHEK 293T cellsMyocilin geneMutant proteinsSecretory pathwayUnidentified functionExtracellular proteinsMutantsEndoproteolytic processingRecombinant mutantsMyocilin secretionCellular fractionsHeterozygous expressionMyocilinProteinUnknown mechanismExtracellular amountSDS-PAGEHeterozygous state
1994
cDNA from human ocular ciliary epithelium homologous to βig‐h3 is preferentially expressed as an extracellular protein in the corneal epithelium
Escribano J, Hernando N, Ghosh S, Coca‐Prados M, Crabb J. cDNA from human ocular ciliary epithelium homologous to βig‐h3 is preferentially expressed as an extracellular protein in the corneal epithelium. Journal Of Cellular Physiology 1994, 160: 511-521. PMID: 8077289, DOI: 10.1002/jcp.1041600314.Peer-Reviewed Original Research
1992
Comparative Studies of Furosemide Effects on Membrane Potential and Intracellular Chloride Activity in Human and Rabbit Ciliary Epithelium
Chu T, Socci R, Coca-Prados M, Green K. Comparative Studies of Furosemide Effects on Membrane Potential and Intracellular Chloride Activity in Human and Rabbit Ciliary Epithelium. Ophthalmic Research 1992, 24: 83-91. PMID: 1608601, DOI: 10.1159/000267151.Peer-Reviewed Original Research
1989
Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Helbig H, Korbmacher C, Wohlfarth J, Coca-Prados M, Wiederholt M. Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1989, 30: 882-9. PMID: 2470692.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidCalciumCell MembraneChloridesCiliary BodyClone CellsCyclamatesElectric ConductivityEpithelial CellsEpitheliumExtracellular SpaceHumansHydrogen-Ion ConcentrationOuabainPigmentationPotassiumSodiumConceptsIntracellular potentialsCiliary epitheliumCell clonesPresence of SITSPresence of ouabainElectrical membrane propertiesAddition of ouabainCiliary epithelial cellsMM EGTAEpithelial cellsTransient depolarizationMicroelectrode techniquesMembrane voltageCultured humanExtracellular ClCl- conductanceEpitheliumOuabainMembrane potentialIntracellular voltageFree solutionPotential response