1994
Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors.
Hong T, Escribano J, Coca-Prados M. Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors. Investigative Ophthalmology & Visual Science 1994, 35: 4023-30. PMID: 7960584.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearAutoantigensBlotting, WesternCell LineCiliary BodyClone CellsDNA HelicasesDNA-Binding ProteinsDNA, ComplementaryFluorescent Antibody TechniqueHumansImmunoglobulin GKu AutoantigenMaleMiceMice, Inbred BALB CMiddle AgedMolecular WeightNuclear ProteinsPigment Epithelium of EyeRNA, MessengerTissue DonorsConceptsHuman eye donorsPolymyositis overlap syndromeScleroderma-polymyositis overlap syndromeODM-2 cellsHuman ciliary processesCiliary processesOverlap syndromeEye donorsCiliary epitheliumSpecies-specific immune responseAnti-Ku antibodiesVariety of antigensCell linesEpithelium cell linePlaque-purified clonesCiliary epithelial cell lineOcular ciliary epitheliumWestern blot analysisSubunit proteinsEpithelial cell lineCiliary epithelial cellsImmune responseOcular tissues
1989
Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Helbig H, Korbmacher C, Wohlfarth J, Coca-Prados M, Wiederholt M. Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1989, 30: 882-9. PMID: 2470692.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidCalciumCell MembraneChloridesCiliary BodyClone CellsCyclamatesElectric ConductivityEpithelial CellsEpitheliumExtracellular SpaceHumansHydrogen-Ion ConcentrationOuabainPigmentationPotassiumSodiumConceptsIntracellular potentialsCiliary epitheliumCell clonesPresence of SITSPresence of ouabainElectrical membrane propertiesAddition of ouabainCiliary epithelial cellsMM EGTAEpithelial cellsTransient depolarizationMicroelectrode techniquesMembrane voltageCultured humanExtracellular ClCl- conductanceEpitheliumOuabainMembrane potentialIntracellular voltageFree solutionPotential response
1988
Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium
Helbig H, Korbmacher C, Stumpff F, Coca‐Prados M, Wiederholt M. Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium. Journal Of Cellular Physiology 1988, 137: 384-389. PMID: 2848042, DOI: 10.1002/jcp.1041370225.Peer-Reviewed Original ResearchConceptsPigmented ciliary epitheliumMM amilorideAcid loadCiliary epitheliumCell clonesPH-sensitive absorbanceBicarbonate-free mediumRegulation of intracellularCiliary bodyAmilorideExtracellular protonsBovine ciliary bodyEpitheliumConfluent monolayersIntracellularCellsCellular metabolismSlow acidificationAlkalinizationPlastic coverslips