2006
Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium
Ghosh S, Choritz L, Geibel J, Coca-Prados M. Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium. Molecular And Cellular Endocrinology 2006, 253: 63-75. PMID: 16764985, DOI: 10.1016/j.mce.2006.05.002.Peer-Reviewed Original ResearchConceptsP-Akt Ser473Ciliary epitheliumIntracellular cyclic GMP productionInvolvement of somatostatinDose-dependent attenuationReceptor mRNA expressionCyclic GMP productionSST-like immunoreactivityLack of responseOcular ciliary epitheliumAqueous humorSomatostatinNHE activityExchanger activityMRNA expressionCiliary processesNeuroendocrine cellsPC2 antibodyPI3K-AktMultiple intracellularGMP productionConvertases PC1Increases phosphorylationTissue extractsEpithelium
2002
Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium
Cullinane A, Leung P, Ortego J, Coca-Prados M, Harvey B. Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium. British Journal Of Ophthalmology 2002, 86: 676. PMID: 12034692, PMCID: PMC1771149, DOI: 10.1136/bjo.86.6.676.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICalciumCell SizeCells, CulturedCiliary BodyElectrophysiologyHumansLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPigment Epithelium of EyePotassium Channels, Calcium-ActivatedReceptors, AngiotensinRenin-Angiotensin SystemReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsHNPE cellsAngiotensin IINon-pigmented epitheliumSecretory functionAddition of AIIRenin-angiotensin system componentsRenin-angiotensin system expressionIon channel activityPotassium ion channel activityActive BK channelsExpression of angiotensinogenAqueous humor dynamicsTreatment of glaucomaChannel activitySingle-channel patch-clamp techniquesPatch-clamp techniqueBK channel activationLarge-conductance potassium channelsHuman ciliary bodyPolymerase chain reaction analysisReceptor antagonistCurrent armamentariumCiliary bodyChain reaction analysisRAS expressionProtein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line.
Shi C, Barnes S, Coca-Prados M, Kelly ME. Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line. Investigative Ophthalmology & Visual Science 2002, 43: 1525-32. PMID: 11980870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TransformedChloride ChannelsCiliary BodyEnzyme ActivationEnzyme InhibitorsEpithelial CellsPatch-Clamp TechniquesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphoprotein PhosphatasesPhosphorylationProtein-Tyrosine KinasesRabbitsSignal TransductionConceptsProtein tyrosine kinasesProtein tyrosine kinase inhibitorTyrosine kinaseOkadaic acidPP-1Ciliary epithelial cellsHyposmotic stimulationC-Src tyrosine kinasePI3KProtein phosphatase inhibitorPKC inhibitorEpithelial cellsSrc tyrosine kinaseCiliary epithelial cell linePhosphatidylinositol-3 kinase inhibitorKinase inhibitorsProtein phosphatasePhosphatase inhibitorCl- currentEpithelial cell lineNonpigmented ciliary epithelial cellsCell swellingKinasePKC activatorTyrosine kinase inhibitors
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMPExtracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Extracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 448-454. PMID: 12045895, DOI: 10.1076/ceyr.23.6.448.6964.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCalciumCalcium-Transporting ATPasesCells, CulturedCiliary BodyFluorescent DyesFura-2HumansMicroscopy, FluorescencePigment Epithelium of EyeReceptors, Purinergic P2Receptors, Purinergic P2Y2Signal TransductionSodium-Calcium ExchangerSpectrometry, FluorescenceThapsigarginConceptsNon-pigmented ciliary body epitheliumCiliary body epitheliumHNPE cellsIntracellular storesBody epitheliumFluorescent dye fura-2Extracellular ATP effectP2Y2 receptor activationDye fura-2Calcium signalsATPase pumpPresence of thapsigarginCapacitative calcium entrySodium-free solutionIntracellular calciumPurinergic receptorsFura-2Therapeutic targetCalcium entryPotency orderReceptor activationSustained increaseL-typeExtracellular ATPOscillatory increases
1994
Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine
1992
Regulatory volume decrease by cultured non-pigmented ciliary epithelial cells
Civan M, Peterson-Yantorno K, Coca-Prados M, E.Yantorno R. Regulatory volume decrease by cultured non-pigmented ciliary epithelial cells. Experimental Eye Research 1992, 54: 181-191. PMID: 1559547, DOI: 10.1016/s0014-4835(05)80207-6.Peer-Reviewed Original Research