1995
Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics
Ocklind A, Yousufzai S, Ghosh S, Coca-Prados M, St Jernschantz J, Abdel-Latif A. Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics. Experimental Eye Research 1995, 61: 535-545. PMID: 8654496, DOI: 10.1016/s0014-4835(05)80047-8.Peer-Reviewed Original ResearchConceptsSignal transduction pathwaysCat iris sphincter smooth muscle cellsSmooth muscle alpha-actinMuscle alpha-actinImmortalized cellsTransduction pathwaysSmooth muscle cellsMuscle cellsAlpha-actinImportant model systemSV40 virusLarge T antigenImmortalized cell culturesMlc proteinNormal muscle cellsTransformation phenotypeSuccessful immortalizationLow-serum mediumCellular DNAContact inhibitionPopulation doublingsSV-CISM-2 cellsT antigenViral transformationArachidonic acid
1994
Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine
1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform
1989
Receptor-mediated phosphoinositide hydrolysis in human ocular ciliary epithelial cells.
Wax M, Coca-Prados M. Receptor-mediated phosphoinositide hydrolysis in human ocular ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1989, 30: 1675-9. PMID: 2545648.Peer-Reviewed Original ResearchConceptsPI hydrolysisReceptor-mediated phosphoinositide hydrolysisEpithelial cellsSelective muscarinic antagonistsInositol phosphatesAccumulation of inositolHydrolysis of phosphoinositidesOcular ciliary epitheliumAFDX-116M3 subtypeMuscarinic antagonistMuscarinic receptorsPutative neurotransmittersEC50 valuesPharmacologic characteristicsCiliary epithelial cellsPeripheral tissuesSpecific antagonistPI breakdownPhosphoinositide hydrolysisArginine vasopressinNonpigmented layerOcular ciliary epithelial cellsCiliary epitheliumMicroCi/
1986
Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
Coca-Prados M, Wax M. Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8754-8758. PMID: 3022303, PMCID: PMC387010, DOI: 10.1073/pnas.83.22.8754.Peer-Reviewed Original ResearchConceptsSimian virus 40Ciliary epithelial cellsSouthern blot analysisLarge tumor antigenHuman ciliary epithelial cellsEpithelial cellsFunctional conservationProtein phosphorylationDirect bindingCell DNAParent cellsBlot analysisCell proliferationCellular cAMP productionReceptor-mediated processEpithelial cell DNAViral DNAImmunoprecipitationDNACAMP productionSelective drugsCell clonesMembrane preparationsCellsReceptors
1982
Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion
Coca-Prados M, Yu H, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion. Journal Of Virology 1982, 44: 603-609. PMID: 6292520, PMCID: PMC256304, DOI: 10.1128/jvi.44.2.603-609.1982.Peer-Reviewed Original ResearchConceptsSimian virus 40 nucleoprotein complexesNucleoprotein complexesSV40 chromatinNuclease digestionDNA fragmentsCapsid shellMature virionsNucleosome repeat lengthMicrococcal nuclease digestionGenome-sized DNASV40 nucleoprotein complexesIntracellular formNovel DNA fragmentsStructure of DNAAssembly intermediatesNucleosomal DNA fragmentsMicrococcal nucleaseVirion assemblyMild digestionSV40 virionsChromatinFraction of intermediatesVirion assembly intermediatesVirion maturationCapsid protein
1980
Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications
Coca-Prados M, Vidali G, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications. Journal Of Virology 1980, 36: 353-360. PMID: 6253661, PMCID: PMC353651, DOI: 10.1128/jvi.36.2.353-360.1980.Peer-Reviewed Original ResearchMeSH KeywordsAcetylationAnimalsCell LineChromatinHaplorhiniHistonesSimian virus 40Viral ProteinsVirionConceptsSimian virus 40 chromatinSimian virus 40 nucleoprotein complexesNucleoprotein complexesHistone modificationsAssembly intermediatesModification patternsMature virionsVirion assembly intermediatesCapsid proteinModification of histonesHistone modification patternsVirion assembly pathwayViral nucleoprotein complexesPulse-chase experimentsHost chromatinHistone patternsAssembly pathwayChromatinHistonesVirion assemblyPolyacrylamide gel electrophoresisAcetylation activityIntracellular formGel electrophoresisViral RNAEnzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA.
Ford J, Coca-Prados M, Hsu M. Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA. Journal Of Biological Chemistry 1980, 255: 7544-7547. PMID: 6249806, DOI: 10.1016/s0021-9258(19)43861-1.Peer-Reviewed Original ResearchConceptsEukaryotic gene expressionSimian virus 40 DNAEukaryotic cellsEukaryotic DNASimian virus 40Gene expressionHost DNAViral genomeCell DNADNAMonkey kidney cellsIntracellular viral DNAEnzymatic analysisViral DNAKidney cellsGenomeCellsAcid hydrolysis procedureThymineExact quantitationDeaminationExpressionEnzymatic methodVivo
1979
Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly
Coca-Prados M, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly. Journal Of Virology 1979, 31: 199-208. PMID: 228054, PMCID: PMC353436, DOI: 10.1128/jvi.31.1.199-208.1979.Peer-Reviewed Original ResearchIntracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells
Fernandez-Munoz R, Coca-Prados M, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells. Journal Of Virology 1979, 29: 612-623. PMID: 219250, PMCID: PMC353194, DOI: 10.1128/jvi.29.2.612-623.1979.Peer-Reviewed Original Research